{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T11:47:31Z","timestamp":1788004051319,"version":"build-2784847793"},"reference-count":64,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007288","name":"Mahasarakham University","doi-asserted-by":"publisher","award":["6408038"],"award-info":[{"award-number":["6408038"]}],"id":[{"id":"10.13039\/501100007288","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Mahidol University, Amnat Charoen Campus, the Center of Excellence for Innovation in Chemistry (PERCH-CIC)","award":["6408038"],"award-info":[{"award-number":["6408038"]}]},{"name":"Ministry of Higher Education, Science, Research and Innovation","award":["6408038"],"award-info":[{"award-number":["6408038"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A simple and reliable dispersive liquid-liquid microextraction (DLLME) coupled with smartphone-based digital images using crude peroxidase extracts from cassia bark (Senna siamea Lam.) was proposed to determine carbaryl residues in Andrographis paniculata herbal medicines. The method was based on the reaction of 1-naphthol (hydrolysis of carbaryl) with 4-aminoantipyrine (4-AP) in the presence of hydrogen peroxide, using peroxidase enzyme simple extracts from cassia bark as biocatalysts under pH 6.0. The red product, after preconcentration by DLLME using dichloromethane as extraction solvent, was measured for blue intensity by daily life smartphone-based digital image analysis. Under optimized conditions, good linearity of the calibration graph was found at 0.10\u20130.50 mg\u00b7L\u22121 (r2 = 0.9932). Limits of detection (LOD) (3SD\/slope) and quantification (LOQ) (10SD\/slope) were 0.03 and 0.09 mg\u00b7L\u22121, respectively, with a precision of less than 5%. Accuracy of the proposed method as percentage recovery gave satisfactory results. The proposed method was successfully applied to analyze carbaryl in Andrographis paniculata herbal medicines. Results agreed well with values obtained from the HPLC-UV method at 95% confidence level. This was simple, convenient, reliable, cost-effective and traceable as an alternative method for the determination of carbaryl.<\/jats:p>","DOI":"10.3390\/molecules27103261","type":"journal-article","created":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T00:18:11Z","timestamp":1653005891000},"page":"3261","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A Simple and Reliable Dispersive Liquid-Liquid Microextraction with Smartphone-Based Digital Images for Determination of Carbaryl Residues in Andrographis paniculata Herbal Medicines Using Simple Peroxidase Extract from Senna siamea Lam. Bark"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7876-4949","authenticated-orcid":false,"given":"Sam-ang","family":"Supharoek","sequence":"first","affiliation":[{"name":"Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand"},{"name":"Department of Medical Science, Mahidol University, Amnatcharoen Campus, Amnat Charoen 37000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Watsaka","family":"Siriangkhawut","sequence":"additional","affiliation":[{"name":"Creative Chemistry and Innovation Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5871-8291","authenticated-orcid":false,"given":"Kate","family":"Grudpan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Analytical Science and Technology for Biodiversity-Based Economic and Society, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6938-9208","authenticated-orcid":false,"given":"Kraingkrai","family":"Ponhong","sequence":"additional","affiliation":[{"name":"Creative Chemistry and Innovation Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Babich, O., Sukhikh, S., Prosekov, A., Asyakina, L., and Ivanova, S. (2020). Medicinal Plants to Strengthen Immunity during a Pandemic. Pharmaceuticals, 13.","DOI":"10.3390\/ph13100313"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"153361","DOI":"10.1016\/j.phymed.2020.153361","article-title":"Herbal immune-boosters: Substantial warriors of pandemic Covid-19 battle","volume":"85","author":"Khanna","year":"2020","journal-title":"Phytomedicine"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/fft2.107","article-title":"Medicinal plants as immune booster in the palliative management of viral diseases: A perspective on coronavirus","volume":"3","author":"Ogunrinola","year":"2022","journal-title":"Food Front."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s11101-021-09756-2","article-title":"Which East Asian herbal medicines can decrease viral infections?","volume":"21","author":"Kuchta","year":"2021","journal-title":"Phytochem. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"846740","DOI":"10.1155\/2013\/846740","article-title":"Experimental and Clinical Pharmacology of Andrographis paniculata and Its Major Bioactive Phytoconstituent Andrographolide","volume":"2013","author":"Jayakumar","year":"2013","journal-title":"Evid. Based Complementary Altern. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1515\/jbcpp-2014-0051","article-title":"Antioxidant, antinociceptive and anti-inflammatory properties of the aqueous and ethanolic leaf extracts of Andrographis paniculata in some laboratory animals","volume":"26","author":"Adedapo","year":"2015","journal-title":"J. Basic Clin. Physiol. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mussard, E., Cesaro, A., Lespessailles, E., Legrain, B., Berteina-Raboin, S., and Toumi, H. (2019). Andrographolide, A Natural Antioxidant: An Update. Antioxidants, 8.","DOI":"10.3390\/antiox8120571"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1021\/np50097a002","article-title":"Immunostimulant Agents from Andrographis paniculata","volume":"56","author":"Puri","year":"1993","journal-title":"J. Nat. Prod."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"67","DOI":"10.4308\/hjb.22.2.67","article-title":"Antiviral and Immunostimulant Activities of Andrographis paniculata","volume":"22","author":"Churiyah","year":"2015","journal-title":"HAYATI J. Biosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1046\/j.1359-4117.2003.01090.x","article-title":"Andrographolide, a potential cancer therapeutic agent isolated from Andrographis paniculata","volume":"3","author":"Rajagopal","year":"2003","journal-title":"J. Exp. Ther. Oncol."},{"key":"ref_11","first-page":"1667","article-title":"Anticancer activity of Andrographis paniculata leaves extract against neuroblastima (IMR-32) and human colon (HT-29) cancer cell line","volume":"4","author":"Shanmugam","year":"2015","journal-title":"World J. Pharm. Pharm. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"113936","DOI":"10.1016\/j.jep.2021.113936","article-title":"Anticancer potential of andrographolide from Andrographis paniculata (Burm.f.) Nees and its mechanisms of action","volume":"272","author":"Malik","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Hu, X.-Y., Wu, R.-H., Logue, M., Blondel, C., Lai, L.Y.W., Stuart, B., Flower, A., Fei, Y.-T., Moore, M., and Shepherd, J. (2017). Andrographis paniculata (Chu\u0101n X\u012bn Li\u00e1n) for symptomatic relief of acute respiratory tract infections in adults and children: A systematic review and meta-analysis. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0181780"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/(SICI)1099-1573(200003)14:2<73::AID-PTR577>3.0.CO;2-#","article-title":"Pesticide residues in medicinal plants and phytomedicines","volume":"14","author":"Zuin","year":"2000","journal-title":"Phytother. Res."},{"key":"ref_15","unstructured":"Wexler, P. (2014). Carbaryl. Encyclopedia of Toxicology, Academic Press. [3rd ed.]."},{"key":"ref_16","unstructured":"Wexler, P. (2005). Carbaryl. Encyclopedia of Toxicology, Elsevier. [2nd ed.]."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Srivastava, A.K., and Kesavachandran, C. (2019). Health Effects of Pesticides, CRC Press. [1st ed.].","DOI":"10.1201\/9780429058219"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/0379-0738(90)90268-4","article-title":"High performance liquid chromatographic method for the analysis of organophosphorus and carbamate pesticides","volume":"48","author":"Sharma","year":"1990","journal-title":"Forensic Sci. Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.aca.2008.11.020","article-title":"Application of dispersive liquid\u2013liquid microextraction for the analysis of triazophos and carbaryl pesticides in water and fruit juice samples","volume":"632","author":"Fu","year":"2009","journal-title":"Anal. Chim. Acta"},{"key":"ref_20","first-page":"2778","article-title":"Determination of carbamates in soils by liquid chromatography coupled with on-line postcolumn UV irradiation and chemiluminescence detection","volume":"13","author":"Bermejo","year":"2018","journal-title":"Arab. J. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0021-9673(01)88453-3","article-title":"Applications of thermospray liquid chromatography-mass spectrometry in photochemical studies of pesticides in water","volume":"554","author":"Durand","year":"1991","journal-title":"J. Chromatogr. A"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.talanta.2005.11.012","article-title":"Application of matrix solid phase dispersion to the determination of imidacloprid, carbaryl, aldicarb, and their main metabolites in honeybees by liquid chromatography\u2013mass spectrometry detection","volume":"69","author":"Totti","year":"2006","journal-title":"Talanta"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Guan, J., Gao, W., Lv, S., and Ge, M. (2018). Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS\/MS QTRAP System. Molecules, 23.","DOI":"10.3390\/molecules23102496"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0021-9673(77)80008-3","article-title":"Gas chromatographic determination of microamounts of carbaryl and 1-napththol in natural water as sources of water supplies","volume":"144","author":"Nagasawa","year":"1977","journal-title":"J. Chromatogr. A"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S0021-9673(01)88658-1","article-title":"Determination of carbaryl and 1-naphthol in english apples and strawberries by combined gas chromatography-fluorescence spectrometry","volume":"547","author":"Bagheri","year":"1991","journal-title":"J. Chromatogr. A"},{"key":"ref_26","first-page":"341","article-title":"Analysis of carbaryl and other pesticides by capillary electrophoresis","volume":"20","author":"Zhang","year":"2002","journal-title":"Chin. J. Chromatogr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8832","DOI":"10.1021\/jf801645m","article-title":"Development of a Capillary Electrophoresis-Based Immunoassay with Laser-Induced Fluorescence for the Detection of Carbaryl in Rice Samples","volume":"56","author":"Zhang","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.foodchem.2013.11.046","article-title":"Electrochemical nonenzymatic sensor based on CoO decorated reduced graphene oxide for the simultaneous determination of carbofuran and carbaryl in fruits and vegetables","volume":"151","author":"Wang","year":"2014","journal-title":"Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.jfca.2014.12.010","article-title":"Electrochemical analysis of carbaryl in fruit samples on graphene oxide-ionic liquid composite modified electrode","volume":"40","author":"Liu","year":"2015","journal-title":"J. Food Compos. Anal."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.jare.2017.08.002","article-title":"Electrochemical sensor based on low silica X zeolite modified carbon paste for carbaryl determination","volume":"8","author":"Salih","year":"2017","journal-title":"J. Adv. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3005","DOI":"10.1007\/s12161-018-1280-4","article-title":"Modified 3D Graphene-Au as a Novel Sensing Layer for Direct and Sensitive Electrochemical Determination of Carbaryl Pesticide in Fruit, Vegetable, and Water Samples","volume":"11","author":"Rahmani","year":"2018","journal-title":"Food Anal. Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.saa.2006.08.003","article-title":"Determination of carbaryl and its metabolite 1-naphthol in water samples by fluorescence spectrophotometer after anionic surfactant micelle-mediated extraction with sodium dodecylsulfate","volume":"67","author":"Jia","year":"2007","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4966","DOI":"10.1021\/acs.jafc.5b01072","article-title":"Development of Fluorescence Sensing Material Based on CdSe\/ZnS Quantum Dots and Molecularly Imprinted Polymer for the Detection of Carbaryl in Rice and Chinese Cabbage","volume":"63","author":"Zhang","year":"2015","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1039\/an9871200075","article-title":"Spectrophotometric determination of carbaryl and propoxur using aminophenols and phenylenediamine","volume":"112","author":"Sastry","year":"1987","journal-title":"Analyst"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1039\/an9952001799","article-title":"Simple spectrophotometric method for the determination of carbaryl in soil and insecticide formulations","volume":"120","author":"Mathew","year":"1995","journal-title":"Analyst"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1081\/AL-200050334","article-title":"Preparation of Reagents for the Sensitive Spectrophotometric Determination of Carbaryl in Environmental Samples","volume":"38","author":"Kumar","year":"2005","journal-title":"Anal. Lett."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.saa.2014.12.031","article-title":"Spectrophotometric determination of trace carbaryl in water and grain samples by inhibition of the rhodamine-B oxidation","volume":"139","author":"Gupta","year":"2015","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1039\/an9790401185","article-title":"Spectrophotometric method for the determination of residues of carbaryl in water","volume":"104","author":"Handa","year":"1979","journal-title":"Analyst"},{"key":"ref_39","first-page":"29","article-title":"Comparison between Digital Image Processing and Spectrophotometric Measurements Methods. Application in Electrophoresis Interpretation","volume":"28","author":"Haifa","year":"2011","journal-title":"Appl. Med. Inf."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1111\/j.1365-2842.2005.01560.x","article-title":"Comparison of digital and spectrophotometric measurements of colour shade guides","volume":"33","author":"Cal","year":"2006","journal-title":"J. Oral Rehabil."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"127971","DOI":"10.1016\/j.foodchem.2020.127971","article-title":"Digital image colorimetry detection of carbaryl in food samples based on liquid phase microextraction coupled with a microfluidic thread-based analytical device","volume":"337","author":"Jing","year":"2020","journal-title":"Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1134\/S1061934818070067","article-title":"Use of Contrast of Digital Photo Images for the Determination of the Turbidity of Liquids","volume":"73","author":"Golovanov","year":"2018","journal-title":"J. Anal. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.proenv.2014.03.037","article-title":"Determination of Chromium and Iron Using Digital Image-based Colorimetry","volume":"20","author":"Firdaus","year":"2014","journal-title":"Procedia Environ. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"12141","DOI":"10.1021\/acsomega.8b01270","article-title":"Color Space Transformation-Based Smartphone Algorithm for Colorimetric Urinalysis","volume":"3","author":"Yang","year":"2018","journal-title":"ACS Omega"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.bios.2017.09.028","article-title":"Point-of-care testing for streptomycin based on aptamer recognizing and digital image colorimetry by smartphone","volume":"100","author":"Lin","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.talanta.2019.01.032","article-title":"Analytical aspects of smart (phone) fluorometric measurements","volume":"197","author":"Granica","year":"2019","journal-title":"Talanta"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.foodchem.2018.01.021","article-title":"A biodegradable colorimetric film for rapid low-cost field determination of formaldehyde contamination by digital image colorimetry","volume":"249","author":"Wongniramaikul","year":"2018","journal-title":"Food Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2873","DOI":"10.1039\/C9AN02294E","article-title":"A smartphone-based biomedical sensory system","volume":"145","author":"Zhao","year":"2020","journal-title":"Analyst"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1098\/rsif.2011.0809","article-title":"Using digital photography to implement the McFarland method","volume":"9","year":"2012","journal-title":"J. R. Soc. Interface"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/0076-6879(91)02028-8","article-title":"Enzymatic catalysis in organic synthesis","volume":"Volume 202","author":"Wong","year":"1991","journal-title":"Methods in Enzymology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.pisc.2014.02.005","article-title":"Enzyme assays","volume":"1","author":"Bisswanger","year":"2014","journal-title":"Perspect. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.microc.2018.08.048","article-title":"A new method for spectrophotometric determination of carbaryl based on rubber tree bark peroxidase enzymatic reaction","volume":"144","author":"Supharoek","year":"2018","journal-title":"Microchem. J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/s12161-019-01667-5","article-title":"A Cost-Effective Spectrophotometric Method Based on Enzymatic Analysis of Jackfruit Latex Peroxidase for the Determination of Carbaryl and Its Metabolite 1-Napthol Residues in Organic and Chemical-Free Vegetables","volume":"13","author":"Didpinrum","year":"2019","journal-title":"Food Anal. Methods"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1007\/s13738-016-0845-7","article-title":"Employing peroxidase from Thai indigenous plants for the application of hydrogen peroxide assay","volume":"13","author":"Ponhong","year":"2016","journal-title":"J. Iran. Chem. Soc."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0003-2670(99)00455-9","article-title":"Zucchini crude extract-palladium-modified carbon paste electrode for the determination of hydroquinone in photographic developers","volume":"398","author":"Vieira","year":"1999","journal-title":"Anal. Chim. Acta"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.biotechadv.2010.09.001","article-title":"Enzymatic and whole cell catalysis: Finding new strategies for old processes","volume":"29","year":"2011","journal-title":"Biotechnol. Adv."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2342","DOI":"10.1016\/j.chroma.2009.11.088","article-title":"Evolution of dispersive liquid\u2013liquid microextraction method","volume":"1217","author":"Rezaee","year":"2010","journal-title":"J. Chromatogr. A"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1039\/C2AY25838B","article-title":"Enhancement of sensitivity for the spectrophotometric determination of carbaryl using dispersive liquid microextraction combined with dispersive \u03bc-solid phase extraction","volume":"5","author":"Mukdasai","year":"2012","journal-title":"Anal. Methods"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.jscs.2011.11.005","article-title":"The recent developments in dispersive liquid\u2013liquid microextraction for preconcentration and determination of inorganic analytes","volume":"18","author":"Emara","year":"2014","journal-title":"J. Saudi Chem. Soc."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1002\/j.1551-8833.1967.tb03426.x","article-title":"Spectrophotometric Determination of Sevin in Natural Waters","volume":"59","author":"Aly","year":"1967","journal-title":"J. Am. Water Work. Assoc."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chroma.2006.03.007","article-title":"Determination of organic compounds in water using dispersive liquid\u2013liquid microextraction","volume":"1116","author":"Rezaee","year":"2006","journal-title":"J. Chromatogr. A"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"4040165","DOI":"10.1155\/2016\/4040165","article-title":"Dispersive Liquid-Liquid Microextraction in the Analysis of Milk and Dairy Products: A Review","volume":"2016","author":"Quigley","year":"2016","journal-title":"J. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/S0165-9936(03)01007-0","article-title":"Developments in liquid-phase microextraction","volume":"22","author":"Psillakis","year":"2003","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"36494","DOI":"10.1039\/D1RA04321H","article-title":"A newly designed sticker-plastic sheet platform and smartphone-based digital imaging for protein assay in food samples with downscaling Kjeldahl digestion","volume":"11","author":"Didpinrum","year":"2021","journal-title":"RSC Adv."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/10\/3261\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:14:57Z","timestamp":1760138097000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/10\/3261"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,19]]},"references-count":64,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["molecules27103261"],"URL":"https:\/\/doi.org\/10.3390\/molecules27103261","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,19]]}}}