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Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism.<\/jats:p>","DOI":"10.3390\/molecules27103343","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T03:16:55Z","timestamp":1653362215000},"page":"3343","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5144-2138","authenticated-orcid":false,"given":"Viktoriia","family":"Korniienko","sequence":"first","affiliation":[{"name":"Biomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2217-3717","authenticated-orcid":false,"given":"Yevheniia","family":"Husak","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, Ukraine"},{"name":"Faculty of Chemistry, Silesian University of Technology, 44-100 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Radwan-Prag\u0142owska","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktoriia","family":"Holubnycha","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yevhen","family":"Samokhin","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8040-7457","authenticated-orcid":false,"given":"Anna","family":"Yanovska","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Varava","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kateryna","family":"Diedkova","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0141ukasz","family":"Janus","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9372-7791","authenticated-orcid":false,"given":"Maksym","family":"Pogorielov","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Sumy State University, 2, Rymsky-Korsakov Str., 40007 Sumy, Ukraine"},{"name":"Institute of Atomic Physics and Spectroscopy, University of Latvia, Jelgavas iela 3, LV-1004 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bahamondez-Canas, T.F., Heersema, L.A., and Smyth, H.D.C. 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