{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T00:26:03Z","timestamp":1760228763600,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T00:00:00Z","timestamp":1653264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021A1515012458","19KJA430013","31800667"],"award-info":[{"award-number":["2021A1515012458","19KJA430013","31800667"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Project of Natural Science Foundation of the Higher Education Institutions of Jiangsu Province","award":["2021A1515012458","19KJA430013","31800667"],"award-info":[{"award-number":["2021A1515012458","19KJA430013","31800667"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2021A1515012458","19KJA430013","31800667"],"award-info":[{"award-number":["2021A1515012458","19KJA430013","31800667"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Anthracycline dutomycin is a tetracyclic quinone glycoside produced by Streptomyces minoensis NRRL B-5482. SW91 is a C-12 demethylated dutomycin derivative, which was identified in our previous research. In vitro cytotoxicity and apoptosis assays of these two compounds were conducted to demonstrate their antiproliferation activities. The results showed that both dutomycin and SW91 block cells at the S phase, whereas dutomycin shows more significant inhibition of cell growth. Their interactions with calf thymus DNA (CT-DNA) were investigated, with dutomycin exhibiting higher binding affinity. The molecular docking demonstrated that the 12-methyl group makes dutomycin attach to the groove of DNA. These findings suggest that dutomycin has binding higher affinity to DNA and impairs DNA replication resulting in more significant antitumor activity.<\/jats:p>","DOI":"10.3390\/molecules27103348","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T03:16:55Z","timestamp":1653362215000},"page":"3348","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Critical Role of 12-Methyl Group of Anthracycline Dutomycin to Its Antiproliferative Activity"],"prefix":"10.3390","volume":"27","author":[{"given":"Ruoxuan","family":"Xu","sequence":"first","affiliation":[{"name":"Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Ministry of Education, Guangzhou 510000, China"},{"name":"Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicine, Guangzhou 510000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinghang","family":"Hu","sequence":"additional","affiliation":[{"name":"Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Ministry of Education, Guangzhou 510000, China"},{"name":"Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicine, Guangzhou 510000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinlian","family":"Lin","sequence":"additional","affiliation":[{"name":"Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Ministry of Education, Guangzhou 510000, China"},{"name":"Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicine, Guangzhou 510000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Tang","sequence":"additional","affiliation":[{"name":"Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Ministry of Education, Guangzhou 510000, China"},{"name":"Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicine, Guangzhou 510000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruoting","family":"Zhan","sequence":"additional","affiliation":[{"name":"Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Ministry of Education, Guangzhou 510000, China"},{"name":"Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicine, Guangzhou 510000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guiyou","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Chemical Engineering, Jiangsu Second Normal University, Nanjing 211200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5024-4164","authenticated-orcid":false,"given":"Lei","family":"Sun","sequence":"additional","affiliation":[{"name":"Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China"},{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Ministry of Education, Guangzhou 510000, China"},{"name":"Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicine, Guangzhou 510000, China"},{"name":"School of Life Sciences and Chemical Engineering, Jiangsu Second Normal University, Nanjing 211200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.2174\/1389200216888150915112039","article-title":"Role of drug metabolism in the cytotoxicity and clinical efficacy of anthracyclines","volume":"16","author":"Edwardson","year":"2015","journal-title":"Curr. 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