{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T06:53:10Z","timestamp":1782370390206,"version":"3.54.5"},"reference-count":47,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T00:00:00Z","timestamp":1653523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Major Natural Science","award":["2019ZF010"],"award-info":[{"award-number":["2019ZF010"]}]},{"name":"Yunnan Major Natural Science","award":["202102AA100009"],"award-info":[{"award-number":["202102AA100009"]}]},{"name":"Yunnan Major Natural Science","award":["YNWR-QNBJ-2020-246"],"award-info":[{"award-number":["YNWR-QNBJ-2020-246"]}]},{"name":"Yunnan Major Natural Science","award":["YNWR-QNBJ-2020-226"],"award-info":[{"award-number":["YNWR-QNBJ-2020-226"]}]},{"name":"Yunnan High-Level Talents Innovation Program","award":["2019ZF010"],"award-info":[{"award-number":["2019ZF010"]}]},{"name":"Yunnan High-Level Talents Innovation Program","award":["202102AA100009"],"award-info":[{"award-number":["202102AA100009"]}]},{"name":"Yunnan High-Level Talents Innovation Program","award":["YNWR-QNBJ-2020-246"],"award-info":[{"award-number":["YNWR-QNBJ-2020-246"]}]},{"name":"Yunnan High-Level Talents Innovation Program","award":["YNWR-QNBJ-2020-226"],"award-info":[{"award-number":["YNWR-QNBJ-2020-226"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The bud of Vaccinium dunalianum Wight has been traditionally consumed as health herbal tea by \u201cYi\u201d people in Yunnan Province, China, which was locally named \u201cQue Zui tea\u201d. This paper studied the chemical constituents of five fractions from Vaccinium dunalianum, and their enzyme inhibitory effects of \u03b1-glucosidase and pancreatic lipase, antioxidant activity, and cytoprotective effects on H2O2-induced oxidative damage in HepG2 cells. The methanol extract of V. dunalianum was successively partitioned with petroleum ether (PF), chloroform (CF), ethyl acetate (EF), n-butanol (BF), and aqueous (WF) to obtain five fractions. The chemical profiling of the five fractions was analyzed by ultra-high-performance liquid chromatography coupled with a tandem mass spectrometry (UHPLC-MS\/MS), and 18 compounds were tentatively identified. Compared to PF, CF, BF and WF, the EF revealed the highest total phenols (TPC) and total flavonoids (TFC), and displayed the strongest enzyme inhibition ability (\u03b1-glucosidase and pancreatic lipase) and antioxidant capacity (DPPH, ABTS and FRAP). Furthermore, these five fractions, especially EF, could effectively inhibit reactive oxygen species (ROS) production and cell apoptosis on H2O2-induced oxidative damage protection in HepG2 cells. This inhibitory effect might be caused by the up-regulation of intracellular antioxidant enzyme activity (CAT, SOD, and GSH). The flavonoids and phenolic acids of V. dunalianum might be the bioactive substances responsible for enzyme inhibitory, antioxidant, and cytoprotective activities.<\/jats:p>","DOI":"10.3390\/molecules27113432","type":"journal-article","created":{"date-parts":[[2022,5,29]],"date-time":"2022-05-29T23:43:03Z","timestamp":1653867783000},"page":"3432","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Phenolic Constituents, Antioxidant and Cytoprotective Activities, Enzyme Inhibition Abilities of Five Fractions from Vaccinium dunalianum Wight"],"prefix":"10.3390","volume":"27","author":[{"given":"Chang-Shu","family":"Cheng","sequence":"first","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing-Hui","family":"Gu","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Ke","family":"Zhang","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Hong","family":"Tao","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian-Rui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Xin","family":"Cao","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5553-8399","authenticated-orcid":false,"given":"Gui-Guang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Fang","family":"Lai","sequence":"additional","affiliation":[{"name":"Yunnan Institute for Food and Drug Control, Kunming 650106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ping","family":"Liu","sequence":"additional","affiliation":[{"name":"Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.tplants.2016.08.002","article-title":"ROS Are Good","volume":"22","author":"Mittler","year":"2017","journal-title":"Trends Plant Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/nure.12091","article-title":"Seaweed and human health","volume":"72","author":"Brown","year":"2014","journal-title":"Nutr. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1016\/S0002-9343(00)00412-5","article-title":"The evolution of free radicals and oxidative stress","volume":"108","author":"McCord","year":"2000","journal-title":"Am. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zhao, T., Sun, M., Kong, L., Xue, Q., Wang, Y., Wang, Y., Khan, A., Cao, J., and Cheng, G. (2021). Bioactivity-Guided Isolation of Phytochemicals from Vaccinium dunalianum Wight and Their Antioxidant and Enzyme Inhibitory Activities. Molecules, 26.","DOI":"10.3390\/molecules26072075"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"404","DOI":"10.3945\/an.113.005603","article-title":"Effect of citrus flavonoids, naringin and naringenin, on metabolic syndrome and their mechanisms of action","volume":"5","author":"Alam","year":"2014","journal-title":"Adv. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.mrfmmm.2005.03.023","article-title":"Phenolics as potential antioxidant therapeutic agents: Mechanism and actions","volume":"579","author":"Soobrattee","year":"2005","journal-title":"Mutat. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"257","DOI":"10.2337\/diacare.19.3.257","article-title":"Oxidative stress and diabetic vascular complications","volume":"19","author":"Giugliano","year":"1996","journal-title":"Diabetes Care"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.jff.2017.12.054","article-title":"New triterpenoids from acorns of Quercus liaotungensis and their inhibitory activity against \u03b1-glucosidase, \u03b1-amylase and protein-tyrosine phosphatase 1B","volume":"41","author":"Xu","year":"2018","journal-title":"J. Funct. Foods"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"154","DOI":"10.2337\/diacare.28.1.154","article-title":"Alpha-glucosidase inhibitors for patients with type 2 diabetes: Results from a Cochrane systematic review and meta-analysis","volume":"28","author":"Lucassen","year":"2005","journal-title":"Diabetes Care"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1039\/C7FO00220C","article-title":"Differential \u03b1-amylase\/\u03b1-glucosidase inhibitory activities of plant-derived phenolic compounds: A virtual screening perspective for the treatment of obesity and diabetes","volume":"8","author":"Rasouli","year":"2017","journal-title":"Food Funct."},{"key":"ref_11","first-page":"180","article-title":"Effect of solvent types and extraction times on phenolic and flavonoid contents and antioxidant activity in leaf extracts of Amomum chinense C","volume":"23","author":"Butsat","year":"2016","journal-title":"Int. Food Res. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"104340","DOI":"10.1016\/j.jff.2020.104340","article-title":"Phenolic compounds in Lycium berry: Composition, health benefits and industrial applications","volume":"77","author":"Jiang","year":"2021","journal-title":"J. Funct. Foods"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Umeno, A., Horie, M., Murotomi, K., Nakajima, Y., and Yoshida, Y. (2016). Antioxidative and Antidiabetic Effects of Natural Polyphenols and Isoflavones. Molecules, 21.","DOI":"10.3390\/molecules21060708"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"111196","DOI":"10.1016\/j.foodres.2022.111196","article-title":"Que Zui tea ameliorates hepatic lipid accumulation and oxidative stress in high fat diet induced nonalcoholic fatty liver disease","volume":"156","author":"Zhang","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.fitote.2011.01.018","article-title":"Antioxidant and prooxidant properties of flavonoids","volume":"82","year":"2011","journal-title":"Fitoterapia"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s00011-009-0037-3","article-title":"Flavonoids as anti-inflammatory agents: Implications in cancer and cardiovascular disease","volume":"58","author":"Villares","year":"2009","journal-title":"Inflamm. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.ijantimicag.2005.09.002","article-title":"Antimicrobial activity of flavonoids","volume":"26","author":"Cushnie","year":"2005","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.ejmech.2017.07.034","article-title":"Recent discoveries of anticancer flavonoids","volume":"142","author":"Raffa","year":"2017","journal-title":"Eur. J. Med. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"131752","DOI":"10.1016\/j.foodchem.2021.131752","article-title":"Phenolic constituents, antioxidant activity and neuroprotective effects of ethanol extracts of fruits, leaves and flower buds from Vaccinium dunalianum Wight","volume":"374","author":"Gao","year":"2022","journal-title":"Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1017\/S0029665111000152","article-title":"Plant secondary metabolites and gut health: The case for phenolic acids","volume":"70","author":"Russell","year":"2011","journal-title":"Proc. Nutr. Soc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1039\/C4RA10590G","article-title":"Involvement of the modulation of cancer cell redox status in the anti-tumoral effect of phenolic compounds","volume":"5","author":"Mendes","year":"2015","journal-title":"RSC Adv."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.fitote.2009.06.009","article-title":"In vivo hypoglycemic effects of phenolics from the root bark of Morus alba","volume":"80","author":"Zhang","year":"2009","journal-title":"Fitoterapia"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"12548","DOI":"10.3390\/molecules181012548","article-title":"Hypolipidemic and antioxidant properties of phenolic compound-rich extracts from white ginseng (Panax ginseng) in cholesterol-fed rabbits","volume":"18","author":"Lee","year":"2013","journal-title":"Molecules"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1007\/s10068-015-0249-y","article-title":"\u03b1-glucosidase inhibitory activities of myricetin in animal models of diabetes mellitus","volume":"24","author":"Kang","year":"2015","journal-title":"Food Sci. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1002\/mnfr.200800563","article-title":"Individual and combined action of pancreatic lipase and pancreatic lipase-related proteins 1 and 2 on native versus homogenized milk fat globules","volume":"53","author":"Berton","year":"2009","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"375","DOI":"10.3109\/10715769509145649","article-title":"The relative antioxidant activities of plant-derived polyphenolic flavonoids","volume":"22","author":"Miller","year":"1995","journal-title":"Free Radic. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1002\/hlca.19960790422","article-title":"Electrochemical Behaviour and Antioxidant Activity of Some Natural Polyphenols","volume":"79","author":"Born","year":"1996","journal-title":"Helv. Chim. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Das, N.P., and Ramanathan, L. (1992). Studies on Flavonoids and Related Compounds as Antioxidants in Food. Lipid-Soluble Antioxidants: Biochemistry and Clinical Applications, Birkh\u00e4user.","DOI":"10.1007\/978-3-0348-7432-8_24"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.foodchem.2017.10.034","article-title":"Study on the effect of reactive oxygen species-mediated oxidative stress on the activation of mitochondrial apoptosis and the tenderness of yak meat","volume":"244","author":"Wang","year":"2018","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s11746-998-0032-9","article-title":"Free radicals, oxidative stress, and antioxidants in human health and disease","volume":"75","author":"Aruoma","year":"1998","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1093\/jn\/134.3.489","article-title":"Glutathione metabolism and its implications for health","volume":"134","author":"Wu","year":"2004","journal-title":"J. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.envpol.2018.06.090","article-title":"Cytotoxicity induced by iodinated haloacetamides via ROS accumulation and apoptosis in HepG-2 cells","volume":"242","author":"Hong","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4727151","DOI":"10.1155\/2017\/4727151","article-title":"Attenuates t-BHP-Induced Oxidative Stress Injury in HepG2 Cells through Antioxidant and Antiapoptosis Activities","volume":"2017","author":"Sun","year":"2017","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.fct.2017.01.022","article-title":"Protective effects of rambutan (Nephelium lappaceum) peel phenolics on H2O2-induced oxidative damages in HepG2 cells and d-galactose-induced aging mice","volume":"108","author":"Zhuang","year":"2017","journal-title":"Food Chem. Toxicol."},{"key":"ref_35","first-page":"722","article-title":"Opinion: Hydrogen peroxide: A metabolic by-product or a common mediator of ageing signals? Nature reviews","volume":"8","author":"Biology","year":"2007","journal-title":"Mol. Cell Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1007\/s11746-999-0182-4","article-title":"Antioxidant activity in soybean oil of extracts from Thompson grape bagasse","volume":"76","year":"1999","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.indcrop.2015.12.033","article-title":"Products, A study on in vitro enzyme inhibitory properties of Asphodeline anatolica: New sources of natural inhibitors for public health problems","volume":"83","author":"Zengin","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1021\/jf204163a","article-title":"Comparative Study of the Effects of Solid-State Fermentation with Three Filamentous Fungi on the Total Phenolics Content (TPC), Flavonoids, and Antioxidant Activities of Subfractions from Oats (Avena sativa L.)","volume":"60","author":"Cai","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Abate, G., Zhang, L., Pucci, M., Morbini, G., Mac Sweeney, E., Maccarinelli, G., Ribaudo, G., Gianoncelli, A., Uberti, D., and Memo, M. (2021). Phytochemical Analysis and Anti-Inflammatory Activity of Different Ethanolic Phyto-Extracts of Artemisia annua L. Biomolecules, 11.","DOI":"10.3390\/biom11070975"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.jff.2017.12.019","article-title":"The free, esterified, and insoluble-bound phenolic profiles of Rhus chinensis Mill. fruits and their pancreatic lipase inhibitory activities with molecular docking analysis","volume":"40","author":"Zhang","year":"2018","journal-title":"J. Funct. Foods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"111433","DOI":"10.1016\/j.indcrop.2019.05.082","article-title":"Phenolic constituents, antioxidant and cytoprotective activities of crude extract and fractions from cultivated artichoke inflorescence","volume":"143","author":"Yang","year":"2020","journal-title":"Ind. Crops Prod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1007\/s13197-011-0251-1","article-title":"Technology, Genesis and development of DPPH method of antioxidant assay","volume":"48","author":"Kedare","year":"2011","journal-title":"J. Food Sci. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.idairyj.2013.09.004","article-title":"Modification of the ferric reducing antioxidant power (FRAP) assay to determine the susceptibility of raw milk to oxidation","volume":"34","author":"Amamcharla","year":"2014","journal-title":"Int. Dairy J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"126512","DOI":"10.1016\/j.foodchem.2020.126512","article-title":"UHPLC-ESI-HRMS\/MS analysis on phenolic compositions of different E Se tea extracts and their antioxidant and cytoprotective activities","volume":"318","author":"Fan","year":"2020","journal-title":"Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.foodchem.2019.03.002","article-title":"Phenolic profiles, antioxidant activities and cytoprotective effects of different phenolic fractions from oil palm (Elaeis guineensis Jacq.) fruits treated by ultra-high pressure","volume":"288","author":"Zhou","year":"2019","journal-title":"Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.clinbiochem.2003.11.015","article-title":"A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation","volume":"37","author":"Erel","year":"2004","journal-title":"Clin. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"113105","DOI":"10.1016\/j.jep.2020.113105","article-title":"In vitro and in vivo anti-inflammatory effects of different extracts from Epigynum auritum through down-regulation of NF-\u03baB and MAPK signaling pathways","volume":"261","author":"Yang","year":"2020","journal-title":"J. Ethnopharmacol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/11\/3432\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:19:07Z","timestamp":1760138347000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/11\/3432"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,26]]},"references-count":47,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["molecules27113432"],"URL":"https:\/\/doi.org\/10.3390\/molecules27113432","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,26]]}}}