{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T10:12:11Z","timestamp":1769422331764,"version":"3.49.0"},"reference-count":61,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Basic Science Institute (KBSI)","award":["C220000"],"award-info":[{"award-number":["C220000"]}]},{"name":"Korea Basic Science Institute (KBSI)","award":["C280320"],"award-info":[{"award-number":["C280320"]}]},{"name":"Korea Basic Science Institute (KBSI)","award":["C210500"],"award-info":[{"award-number":["C210500"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Gynura nepalensis D.C. (family: Asteraceae) has abundant uses in the alternative medicinal practice, and this species is commonly used in the treatment of diabetes, rheumatism, cuts or wounds, asthma, kidney stones, cough, urinary tract bleeding, gall bladder stones, hepatitis, diarrhea, hemorrhoids, constipation, vomiting, fertility problems, blood poisoning, septicemia, skin allergy, indigestion, high cholesterol levels, and so on. This study aims to investigate the hepatoprotective and antioxidant potential of the methanol extract of the Gynura nepalensis D.C. (GNME) along with chemical profiling with phytochemical screening. Moreover, prospective phytocompounds have been screened virtually to present the binding affinity of the bioactive components to the hepatic and oxidative receptors. In the hepatoprotective study, alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), and lipid peroxidation (LP) and total bilirubin (TB) have been assessed, and in the antioxidant study, the DPPH free radical scavenging, total antioxidant flavonoid, and phenolic contents were determined. Moreover, the molecular binding affinity of the bioactive component of the plant has been analyzed using PyRx AutoDock Vina, Chimera, and Discovery Studio software. The plant extract showed dose-dependent hepatoprotective potential (p &lt; 0.05, 0.01, 0.001) as well as strong antioxidant properties. Moreover, hepatoprotective and antioxidant molecular docking studies revealed a result varying from \u22122.90 kcal\/mol to \u221210.1 kcal\/mol. 4,5-dicaffeoylquinic acid and chlorogenic acid revealed the highest binding affinity among the selected molecules. However, the plant showed portent antioxidant and hepatoprotective properties in the in vitro, in vivo, and in silico models, and it is presumed that the hepatoprotective properties of the plant extract have occurred due to the presence of the vast bioactive chemical compounds as well as their antioxidant properties. Therefore, advanced studies are recommended to elucidate the pharmacological properties of the plant extracts.<\/jats:p>","DOI":"10.3390\/molecules27113474","type":"journal-article","created":{"date-parts":[[2022,5,29]],"date-time":"2022-05-29T23:43:03Z","timestamp":1653867783000},"page":"3474","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Chemico-Pharmacological Screening of the Methanol Extract of Gynura nepalensis D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from in vitro, in vivo, and Computer-Aided Studies"],"prefix":"10.3390","volume":"27","author":[{"given":"Nishan","family":"Chakrabarty","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Jagannath University, Dhaka 1100, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1185-7654","authenticated-orcid":false,"given":"Hea-Jong","family":"Chung","sequence":"additional","affiliation":[{"name":"Gwanju Center, Korea Basic Science Institute, Gwanju 61715, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashedul","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Medical School, Jeonbuk National University, Jeonju 54896, Korea"},{"name":"Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7567-4796","authenticated-orcid":false,"given":"Nazim Uddin","family":"Emon","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chattogram 4318, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1831-2278","authenticated-orcid":false,"given":"Safaet","family":"Alam","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Research Division, Bangladesh Council of Scientific and Industrial Research Laboratories (BCSIR), Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka 1205, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed Fazlul","family":"Kabir","sequence":"additional","affiliation":[{"name":"Department of Biology, Georgia State University, Atlanta, GA 30303, USA"},{"name":"Department of Biological Sciences, St. John\u2019s University, Queens, NY 11439, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4950-3210","authenticated-orcid":false,"given":"Md. Minarul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute for Medical Science, Jeonbuk National University Medical School, Jeonju 54907, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8254-6581","authenticated-orcid":false,"given":"Seong-Tshool","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute for Medical Science, Jeonbuk National University Medical School, Jeonju 54907, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tapas","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Gono Bishwabidyalay, Dhaka 1344, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9795-0608","authenticated-orcid":false,"given":"Md. Moklesur Rahman","family":"Sarker","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, State University of Bangladesh, Dhaka 1205, Bangladesh"},{"name":"Pharmacology and Toxicology Research Division, Health Med Science Research Limited, 3\/1 Block F, Lalmatia, Mohammadpur, Dhaka 1207, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6698-2057","authenticated-orcid":false,"given":"Mohammad Manjur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Gono Bishwabidyalay, Dhaka 1344, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kenna, J.G. (2013). Mechanism, pathology, and clinical presentation of hepatotoxicity of anesthetic agents. Drug-Induced Liver Disease, Elsevier.","DOI":"10.1016\/B978-0-12-387817-5.00023-6"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1898","DOI":"10.1248\/bpb.30.1898","article-title":"Protective mechanism of glycyrrhizin on acute liver injury induced by carbon tetrachloride in mice","volume":"30","author":"Lee","year":"2007","journal-title":"Biol. Pharm. Bull."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"785790","DOI":"10.3389\/fphar.2021.785790","article-title":"Novel Therapies for the Treatment of Drug-Induced Liver Injury: A Systematic Review","volume":"12","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_4","first-page":"2196315","article-title":"Hepatoprotective activity of some medicinal plants in Sudan","volume":"2019","author":"Ali","year":"2019","journal-title":"J. Evid. Based Integr. Med."},{"key":"ref_5","first-page":"95","article-title":"Hepatotoxicity of paracetamol and related fatalities","volume":"21","author":"Tittarelli","year":"2017","journal-title":"Eur. Rev. Med. Pharm. Sci."},{"key":"ref_6","first-page":"101","article-title":"Hepatoprotective activity of some plants extract against paracetamol induced hepatotoxicity in rats","volume":"4","author":"Parmar","year":"2010","journal-title":"J. Herb. Med. Toxicol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3807234","DOI":"10.1155\/2020\/3807234","article-title":"Hepatoprotective activity of Leptadenia hastata (asclepiadaceae) on acetaminophen-induced toxicity in mice: In vivo study and characterization of bioactive compounds through molecular docking approaches","volume":"2020","author":"Galani","year":"2020","journal-title":"BioMed Res. Int."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S1383-5718(02)00128-6","article-title":"The comet assay with 8 mouse organs: Results with 39 currently used food additives","volume":"519","author":"Sasaki","year":"2002","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagenesis"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Rashid, U., Khan, M.R., and Sajid, M. (2016). Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat. BMC Complementary Altern. Med., 16.","DOI":"10.1186\/s12906-016-1445-x"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"598","DOI":"10.5530\/pj.2020.12.89","article-title":"In-vitro Antioxidant and In-Vivo Hepatoprotective Activity of Ethenolic Extract of Tectona grandis Bark Against CCl4 Induced Liver Injury in Rats","volume":"12","author":"Bagali","year":"2020","journal-title":"Pharmacogn. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/s13596-020-00429-7","article-title":"Investigation of antinociceptive, anti-inflammatory and thrombolytic activity of Caesalpinia digyna (Rottl.) leaves by experimental and computational approaches","volume":"20","author":"Emon","year":"2020","journal-title":"Adv. Tradit. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1002\/fsn3.2047","article-title":"Antidepressant, anxiolytic, antipyretic, and thrombolytic profiling of methanol extract of the aerial part of Piper nigrum: In vivo, in vitro, and in silico approaches","volume":"9","author":"Emon","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1016\/j.jsps.2020.11.004","article-title":"Pharmacological and computer-aided studies provide new insights into Millettia peguensis Ali (Fabaceae)","volume":"28","author":"Alam","year":"2020","journal-title":"Saudi Pharm. J."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Srivastava, A., Srivastava, P., Pandey, A., Khanna, V., and Pant, A. (2019). Phytomedicine: A potential alternative medicine in controlling neurological disorders. New Look to Phytomedicine, Elsevier.","DOI":"10.1016\/B978-0-12-814619-4.00025-2"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"112185","DOI":"10.1016\/j.biopha.2021.112185","article-title":"Chemical, biological and protein-receptor binding profiling of Bauhinia scandens L. stems provide new insights into the management of pain, inflammation, pyrexia and thrombosis","volume":"143","author":"Emon","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Islam, M.M., Alam, R., Chung, H.-J., Emon, N.U., Fazlul Kabir, M., Rudra, S., Alam, S., Ullah, A., Hong, S.-T., and Aktar Sayeed, M. (2022). Chemical, Pharmacological and Computerized Molecular Analysis of Stem\u2019s Extracts of Bauhinia scandens L. Provide Insights into the Management of Diarrheal and Microbial Infections. Nutrients, 14.","DOI":"10.3390\/nu14020265"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.jep.2003.08.006","article-title":"Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: Part II","volume":"89","author":"Chattopadhyay","year":"2003","journal-title":"J. Ethnopharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"114145","DOI":"10.1016\/j.jep.2021.114145","article-title":"Traditional uses, phytochemistry, pharmacology and toxicology of the genus Gynura (Compositae): A comprehensive review","volume":"276","author":"Meng","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_19","unstructured":"Pe\u00f1a, R.A.D., Gracilla, D.E., Pangilinan, C.R., and Bagunu, J.V. (2018). Gynura Nepalensis DC: A Potential Wonder Medicinal Plant, ResearchGate."},{"key":"ref_20","first-page":"136","article-title":"Further phytochemical screening; non-clinical evaluation of toxic and anti-inflammatory effects of crude aqueous extract of Gynura nepalensis","volume":"1","author":"Aktar","year":"2019","journal-title":"Pharmacol. Online"},{"key":"ref_21","unstructured":"Quattrocchi, U. (2012). CRC World Dictionary of Medicinal and Poisonous Plants: Common Names, Scientific Names, Eponyms, Synonyms, and Etymology (5 Volume Set), CRC Press."},{"key":"ref_22","first-page":"405","article-title":"Medicinal plants used by the folk medicinal practitioners of Bangladesh: A randomized survey in a village of Narayanganj district","volume":"5","author":"Karim","year":"2011","journal-title":"Am. Eur. J. Sustain. Agr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1746-4269-1-11","article-title":"Ethnomedicinal botany of the Apatani in the Eastern Himalayan region of India","volume":"1","author":"Kala","year":"2005","journal-title":"J. Ethnobiol. Ethnomed."},{"key":"ref_24","first-page":"100994","article-title":"Biological and computational studies provide insights into Caesalphinia digyna Rottler stems","volume":"26","author":"Emon","year":"2021","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"461","DOI":"10.3109\/13880200903173593","article-title":"Hepatoprotective activities of two Ethiopian medicinal plants","volume":"48","author":"Umer","year":"2010","journal-title":"Pharm. Biol."},{"key":"ref_26","first-page":"569","article-title":"Hepatoprotective activity of six polyherbal formulations in paracetamol induced liver toxicity in mice","volume":"129","author":"Girish","year":"2009","journal-title":"Indian J. Med. Res."},{"key":"ref_27","first-page":"255","article-title":"Hepatoprotective activity of hydroalcoholic extract of Momordica charantia Linn. leaves against carbon tetra chloride induced hepatopathy in rats","volume":"1","author":"Chaware","year":"2009","journal-title":"Int. J. ChemTech Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1365-2125.2010.03819.x","article-title":"Overdose pattern and outcome in paracetamol-induced acute severe hepatotoxicity","volume":"71","author":"Craig","year":"2011","journal-title":"Br. J. Clin. Pharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1016\/0016-5085(95)90758-0","article-title":"A 7-year experience of severe acetaminophen-induced hepatotoxicity (1987\u20131993)","volume":"109","author":"Makin","year":"1995","journal-title":"Gastroenterology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1002\/lt.21681","article-title":"The utilization of liver transplantation in the management of acute liver failure: Comparison between acetaminophen and non-acetaminophen etiologies","volume":"15","author":"Simpson","year":"2009","journal-title":"Liver Transplant."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ali, S., Gameel, A., Mohamed, A., and Hassan, T. (2011). Hepatoprotective Activity of Capparis Decidua Aqueous and Methanolic Stems Extracts against Carbon Tetrachloride Induced Liver Histological Damage in Rats, University of Khartoum.","DOI":"10.3923\/jpt.2011.62.68"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.lfs.2011.01.025","article-title":"Current issues with acetaminophen hepatotoxicity\u2014A clinically relevant model to test the efficacy of natural products","volume":"88","author":"Jaeschke","year":"2011","journal-title":"Life Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1472-6882-14-406","article-title":"Correlation of antiangiogenic, antioxidant and cytotoxic activities of some Sudanese medicinal plants with phenolic and flavonoid contents","volume":"14","author":"Hassan","year":"2014","journal-title":"BMC Complementary Altern. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1038\/aps.2012.62","article-title":"Differential hepatoprotective mechanisms of rutin and quercetin in CCl4-intoxicated BALB\/cN mice","volume":"33","author":"Jakovac","year":"2012","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-56320-2","article-title":"Assessment of the hepatoprotective effect of developed lipid-polymer hybrid nanoparticles (LPHNPs) encapsulating naturally extracted \u03b2-Sitosterol against CCl4 induced hepatotoxicity in rats","volume":"9","author":"Abdou","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3341","DOI":"10.1007\/s10811-020-02154-9","article-title":"Antioxidant efficacy of (\u2212)-loliolide isolated from Sargassum horneri against AAPH-induced oxidative damage in Vero cells and zebrafish models in vivo","volume":"32","author":"Kim","year":"2020","journal-title":"J. Appl. Phycol."},{"key":"ref_37","first-page":"82","article-title":"Antioxidant Activity-guided Phytochemical Investigation of Artemisia aucheri Boiss.: Isolation of Ethyl Caffeate and a Spinacetin Glycoside","volume":"20","author":"Jahanbani","year":"2021","journal-title":"Iran. J. Pharm. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jep.2007.09.009","article-title":"Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica Roxb. leaves","volume":"115","author":"Jain","year":"2008","journal-title":"J. Ethnopharmacol."},{"key":"ref_39","unstructured":"OECD (2002). Test No. 420: Acute Oral Toxicity\u2014Fixed Dose Procedure, OECD."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Alam, S., Rashid, M.A., Sarker, M.M.R., Emon, N.U., Arman, M., Mohamed, I.N., and Haque, M.R. (2021). Antidiarrheal, antimicrobial and antioxidant potentials of methanol extract of Colocasia gigantea Hook. f. leaves: Evidenced from in vivo and in vitro studies along with computer-aided approaches. BMC Complementary Med. Ther., 21.","DOI":"10.1186\/s12906-021-03290-6"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jep.2009.04.055","article-title":"Hepatoprotective studies on Sida acuta Burm. f","volume":"124","author":"Sreedevi","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4364","DOI":"10.1002\/fsn3.2407","article-title":"Ganoderma applanatum mushroom provides new insights into the management of diabetes mellitus, hyperlipidemia, and hepatic degeneration: A comprehensive analysis","volume":"9","author":"Hossain","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.ab.2018.10.013","article-title":"Preparation of stable tau oligomers for cellular and biochemical studies","volume":"566","author":"Karikari","year":"2019","journal-title":"Anal. Biochem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4001","DOI":"10.1016\/S0021-9258(18)62876-5","article-title":"Protein Catabolism in Rat Liver Homogenates: A Re-Evaluation of the Energy Requirement for Protein Catabolism","volume":"245","author":"Brostrom","year":"1970","journal-title":"J. Biol. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1023\/A:1022853007710","article-title":"Modulatory effect of Henna leaf (Lawsonia inermis) on drug metabolising phase I and phase II enzymes, antioxidant enzymes, lipid peroxidation and chemically induced skin and forestomach papillomagenesis in mice","volume":"245","author":"Dasgupta","year":"2003","journal-title":"Mol. Cell. Biochem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.hpb.2016.06.006","article-title":"Measurement of liver function using hepatobiliary scintigraphy improves risk assessment in patients undergoing major liver resection","volume":"18","author":"Cieslak","year":"2016","journal-title":"HPB"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1111\/j.1478-3231.2008.01777.x","article-title":"Liver failure after partial hepatic resection: Definition, pathophysiology, risk factors and treatment","volume":"28","author":"Dejong","year":"2008","journal-title":"Liver Int."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3923\/ijbc.2009.99.110","article-title":"Antioxidant and hepatoprotective activity of leaf extract of Justicia gendarussa Burm","volume":"3","author":"Krishna","year":"2009","journal-title":"Int. J. Biol. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Hata, A., Fujitani, N., Takeshita, M., Tanaka, C., Matsuda, N., Takaishi, M., Shimokawa Miyama, T., and Hoshi, F. (2021). Comparison of regression for blood ALP levels using methods of the Japan Society of Clinical Chemistry and the International Federation of Clinical Chemistry and Laboratory Medicine in bovine, canine, feline, and human testing. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0253396"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S1056-8719(03)00019-4","article-title":"Optimization of the dose and route of injection, and characterisation of the time course of carbon tetrachloride-induced hepatotoxicity in the rat","volume":"48","author":"Janakat","year":"2002","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"113","DOI":"10.4236\/cm.2017.84009","article-title":"Protective effect of cranberry extracts against oxidative stress and DNA damage induced by diclofenac sodium in kidney of male albino rate","volume":"8","author":"Hassan","year":"2017","journal-title":"Chin. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1093\/clinchem\/20.4.447","article-title":"Detection and Measurement of Total Bilirubin in Serum, with Use of Surfactants as Solubilizing Agents","volume":"20","author":"Pearlman","year":"1974","journal-title":"Clin. Chem."},{"key":"ref_53","first-page":"954136","article-title":"Hepatoprotective effect of pretreatment with Thymus vulgaris essential oil in experimental model of acetaminophen-induced injury","volume":"2014","author":"Grespan","year":"2014","journal-title":"J. Evid. Based Integr. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"176","DOI":"10.2174\/1573411014666180619143729","article-title":"Electrochemical evaluation of the total antioxidant capacity of yam food samples on a polyglycine-glassy carbon modified electrode","volume":"16","author":"Demir","year":"2020","journal-title":"Curr. Anal. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"He, J., Chen, L., Chu, B., and Zhang, C. (2018). Determination of total polysaccharides and total flavonoids in Chrysanthemum morifolium using near-infrared hyperspectral imaging and multivariate analysis. Molecules, 23.","DOI":"10.3390\/molecules23092395"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1038\/nprot.2007.102","article-title":"Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin\u2013Ciocalteu reagent","volume":"2","author":"Ainsworth","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1107\/S0907444903029718","article-title":"Complexed and ligand-free high-resolution structures of urate oxidase (Uox) from Aspergillus flavus: A reassignment of the active-site binding mode","volume":"60","author":"Retailleau","year":"2004","journal-title":"Acta Crystallogr. Sect. D Biol. Crystallogr."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1002\/pro.5560030601","article-title":"Biological meaning, statistical significance, and classification of local spatial similarities in nonhomologous proteins","volume":"3","author":"Alexandrov","year":"1994","journal-title":"Protein Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1021\/ci400060j","article-title":"Computational study on the drug resistance mechanism against HCV NS3\/4A protease inhibitors vaniprevir and MK-5172 by the combination use of molecular dynamics simulation, residue interaction network, and substrate envelope analysis","volume":"54","author":"Xue","year":"2014","journal-title":"J. Chem. Inf. Modeling"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1021\/acschembio.5b01035","article-title":"Stabilizing the CH2 domain of an antibody by engineering in an enhanced aromatic sequon","volume":"11","author":"Chen","year":"2016","journal-title":"ACS Chem. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"5302","DOI":"10.1016\/j.sjbs.2021.05.050","article-title":"Antipyretic activity of Caesalpinia digyna (Rottl.) leaves extract along with phytoconstituent\u2019s binding affinity to COX-1, COX-2, and mPGES-1 receptors: In vivo and in silico approaches","volume":"28","author":"Emon","year":"2021","journal-title":"Saudi J. Biol. Sci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/11\/3474\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:20:18Z","timestamp":1760138418000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/11\/3474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,27]]},"references-count":61,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["molecules27113474"],"URL":"https:\/\/doi.org\/10.3390\/molecules27113474","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,27]]}}}