{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T14:15:47Z","timestamp":1780582547401,"version":"3.54.1"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:00:00Z","timestamp":1653955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Recently, the antimicrobial potential of essential oils extracted from plants has gained extensive research interest, primarily for the development of novel antimicrobial treatments to combat emerging microbial resistance. The current study aims at investigating the antimicrobial activity and chemical composition of essential oil derived from gold coin daisy, which is known as Asteriscus graveolens (EOAG). In this context, a gas chromatography-tandem mass spectrometry (GC-MS) analysis of EOAG was conducted to identify its phytoconstituents. The in vitro antioxidant capacity of EOAG was determined by the use of three tests, namely: 1,1-diphenyl-2-picrylhydrzyl (DPPH), ferric reducing activity power (FRAP), and total antioxidant capacity (TAC). The antimicrobial activity of EOAG against clinically important bacterial (Escherichia coli, K12; Staphylococcus aureus, ATCC 6633; Bacillus subtilis, DSM 6333; and Pseudomonas aeruginosa, CIP A22) and fungal (Candida albicans, ATCC 10231; Aspergillus niger, MTCC 282; Aspergillus flavus, MTCC 9606; and Fusarium oxysporum, MTCC 9913) strains was assessed. Antimicrobial efficacy was determined on solid (inhibition diameter) and liquid media to calculate the minimum inhibitory concentration (MIC). GC\/MS profiling of EOAG revealed that 18 compounds were identified, with a dominance of \u03b1-Thujone (17.92%) followed by carvacrol (14.14%), with a total identification of about 99. 92%. The antioxidant activity of EOAG was determined to have IC50 values of 34.81 \u00b1 1.12 \u00b5g\/mL (DPPH), 89.37 \u00b1 5.02 \u00b5g\/mL (FRAP), and 1048.38 \u00b1 10.23 \u00b5g EAA\/mg (TAC). The antibacterial activity in a solid medium revealed that the largest diameter was recorded in P. aeruginosa (28.47 \u00b1 1.44 mm) followed by S. aureus (27.41 \u00b1 1.54 mm), and the MIC in S. aureus was 12.18 \u00b1 0.98 \u00b5g \/ mL. For the antifungal activity of EOAG, the largest inhibition diameter was found in F. oxysporum (33.62 \u00b1 2.14 mm) followed by C. albicans (26.41 \u00b1 1.90 mm), and the smallest MIC was found in F. oxysporum (18.29 \u00b1 1.21 \u00b5g\/mL) followed by C. albicans (19.39 \u00b1 1.0 \u00b5g\/mL). In conclusion, EOAG can be useful as a natural antimicrobial and antioxidant agent and an alternative to synthetic antibiotics. Hence, they might be utilized to treat a variety of infectious disorders caused by pathogenic microorganisms, particularly those that have gained resistance to standard antibiotics.<\/jats:p>","DOI":"10.3390\/molecules27113539","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T09:24:29Z","timestamp":1653989069000},"page":"3539","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Antioxidant and Antimicrobial Potencies of Chemically-Profiled Essential Oil from Asteriscus graveolens against Clinically-Important Pathogenic Microbial Strains"],"prefix":"10.3390","volume":"27","author":[{"given":"Mohammed M.","family":"Aljeldah","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hafr Al Batin, Hafar al-Batin 31991, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,31]]},"reference":[{"key":"ref_1","unstructured":"Bellakhdar, J. (1997). The Traditional Moroccan Pharmacopoeia: Ancient Arabic Medicine and Popular Knowledge, Ibis Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1515\/chem-2020-0124","article-title":"Citrullus colocynthis (L.) Schrad: Chemical Characterization, Scavenging and Cytotoxic Activities","volume":"18","author":"Bourhia","year":"2020","journal-title":"Open Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/S1779-0123(07)70308-X","article-title":"L\u2019aromath\u00e9rapie et Les Huiles Essentielles","volume":"7","author":"Lardry","year":"2007","journal-title":"Kin\u00e9sith\u00e9rapie La Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.fct.2007.09.106","article-title":"Biological Effects of Essential Oils\u2014A Review","volume":"46","author":"Bakkali","year":"2008","journal-title":"Food Chem. Toxicol."},{"key":"ref_5","first-page":"395","article-title":"Antimicrobial Activity of Essential Oils: A 1976-1986 Literature Review","volume":"53","author":"Janssen","year":"1987","journal-title":"Aspects Test Methods. Planta Med."},{"key":"ref_6","first-page":"9","article-title":"Antioxidant Activities of Some Lamiaceae Plant Extracts. Phytotherapy Research: An International","volume":"20","author":"Erdemoglu","year":"2006","journal-title":"J. Devoted Pharmacol. Toxicol. Eval. Nat. Prod. Deriv."},{"key":"ref_7","first-page":"467","article-title":"In Vitro Antioxidant Activity of Non-Cultivated Vegetables of Ethnic Albanians in Southern Italy","volume":"16","author":"Pieroni","year":"2002","journal-title":"Phytother. Res. Int. J. Devoted Pharmacol. Toxicol. Eval. Nat. Prod. Deriv."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1126\/science.6351251","article-title":"Dietary Carcinogens and Anticarcinogens: Oxygen Radicals and Degenerative Diseases","volume":"221","author":"Ames","year":"1983","journal-title":"Science"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"R713","DOI":"10.1016\/j.cub.2017.05.064","article-title":"Animal Communication: When i\u2019m Calling You, Will You Answer Too?","volume":"27","author":"Vickers","year":"2017","journal-title":"Curr. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.foodcont.2014.12.040","article-title":"Essential Oils as Antimicrobials in Food Systems\u2013A Review","volume":"54","author":"Calo","year":"2015","journal-title":"Food Control"},{"key":"ref_11","first-page":"1694","article-title":"Role_of_Fungal_enzymes_in_Polymer_degradation n: A Mini review","volume":"5","author":"Sumathi","year":"2014","journal-title":"Res. J. Pharm. Biol. Chem. Sci."},{"key":"ref_12","unstructured":"O\u2019Neill, J. (2016). Tackling Drug-Resistant Infections Globally: Final Report and Recommendations, Wellcome Collection. Available online: https:\/\/wellcomecollection.org\/works\/thvwsuba."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/j.biortech.2004.09.006","article-title":"Polyphenol Contents and Antioxidant Activity of Maydis Stigma Extracts","volume":"96","year":"2005","journal-title":"Bioresour. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0169-7439(01)00103-4","article-title":"Chemosystematic Studies of Natural Compounds Isolated from Asteraceae: Characterization of Tribes by Principal Component Analysis","volume":"56","author":"Alvarenga","year":"2001","journal-title":"Chemom. Intell. Lab. Syst."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.procbio.2017.03.004","article-title":"Phytochemical Composition and Biological Activities of Asteriscus Graveolens (Forssk) Extracts","volume":"56","author":"Ramdane","year":"2017","journal-title":"Process Biochem."},{"key":"ref_16","first-page":"1934578X1100601147","article-title":"Antifungal Activity of Essential Oil from Asteriscus Graveolens against Postharvest Phytopathogenic Fungi in Apples","volume":"6","author":"Znini","year":"2011","journal-title":"Nat. Prod. Commun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2190","DOI":"10.1080\/14786419.2019.1666390","article-title":"New sesquiterpenes from Asteriscus graveolens","volume":"35","author":"Achoub","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/s13596-018-0315-0","article-title":"Chemical Composition, Antioxidative, Antimicrobial and Anti-Cancer Activities of Asteriscus graveolens (Forssk) Essential Oil","volume":"18","author":"Aouissi","year":"2018","journal-title":"Orient. Pharm. Exp. Med."},{"key":"ref_19","first-page":"129","article-title":"Corrosion Inhibition Effects of the Essential Oils of Two Asteraceae Plants from South Algeria","volume":"18","author":"Chaib","year":"2020","journal-title":"Inter. J. Chem. Biochem. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/ffj.1794","article-title":"The Essential Oil Composition of Bubonium Graveolens (Forssk.) Maire from the Algerian Sahara","volume":"22","author":"Cheriti","year":"2007","journal-title":"Flavour Fragr. J."},{"key":"ref_21","first-page":"307","article-title":"Chemical Composition and Antimicrobial Activity of the Volatile Oil of Nauplius Graveolens (Forssk.)","volume":"26","author":"Fahmy","year":"2003","journal-title":"Less. J. Environ. Sci."},{"key":"ref_22","unstructured":"Adams, R.P. (2007). Identification of Essential Oil Components by Gas Chromatograpy\/Mass Spectrometry, Allured Publishing Corporation. [4th ed.]."},{"key":"ref_23","first-page":"489","article-title":"Enzymatic and Non-Enzymatic Antioxidants of Calamintha Officinalis Moench Extracts","volume":"3","author":"Moattar","year":"2016","journal-title":"J. Appl. Biotechnol. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"43","DOI":"10.2298\/HEMIND110828068M","article-title":"Phytochemical Screening and Biological Activity of Extracts of Plant Species Halacsya Sendtneri (Boiss.)","volume":"66","year":"2012","journal-title":"D\u00f6rfl. Hem. Ind."},{"key":"ref_25","first-page":"1","article-title":"Antifungal and Antioxidant Activity of Asteriscus graveolens subspodorus Essential Oil","volume":"10","author":"Alilou","year":"2014","journal-title":"J. Nat. Sci. Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Chebbac, K., Ghneim, H.K., El Moussaoui, A., Bourhia, M., El Barnossi, A., Benziane Ouaritini, Z., Salamatullah, A.M., Alzahrani, A., Aboul-Soud, M.A., and Giesy, J.P. (2022). Antioxidant and Antimicrobial Activities of Chemically-Characterized Essential Oil from Artemisia aragonensis Lam. against Drug-Resistant Microbes. Molecules, 27.","DOI":"10.3390\/molecules27031136"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2021\/5902851","article-title":"Chemical Analysis and Antioxidant and Antimicrobial Activity of Essential Oils from Artemisia Negrei, L. Against Drug-Resistant Microbes","volume":"2021","author":"Chebbac","year":"2021","journal-title":"Evid.-Based Complementary Altern. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e1700092","DOI":"10.1002\/cbdv.201700092","article-title":"Chemical Composition and Antimicrobial Activity of Essential Oils from the Aerial Parts of Asteriscus Graveolens (Forssk.) Less. and Pulicaria Incisa (Lam.) DC.: Two Asteraceae Herbs Growing Wild in the Hoggar","volume":"14","author":"Chaib","year":"2017","journal-title":"Chem. Biodivers."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1002\/cbdv.201100118","article-title":"Chemical Diversity of Essential Oils from Asteriscus Graveolens (Forssk.) Less.: Identification of Cis-8-Acetoxychrysanthenyl Acetate as a New Natural Component","volume":"9","author":"Cristofari","year":"2012","journal-title":"Chem. Biodivers."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.indcrop.2014.08.047","article-title":"Chemical Composition of the Essential Oil of Artemisia Hedinii Ostenf. et Pauls. from the Qinghai-Tibetan Plateau","volume":"62","author":"Zhigzhitzhapova","year":"2014","journal-title":"Ind. Crops Prod."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"666005","DOI":"10.3389\/fevo.2021.666005","article-title":"Responses of Withania Frutescens (L.) Pauquy (Solanaceae) Growing in the Mediterranean Area to Changes in the Environmental Conditions: An Approach of Adaptation","volume":"9","author":"Moussaoui","year":"2021","journal-title":"Front. Ecol. Evol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0278-6915(84)90033-4","article-title":"Antioxidant Actions of Thymol, Carvacrol, 6-Gingerol, Zingerone and Hydroxytyrosol","volume":"32","author":"Aeschbach","year":"1994","journal-title":"Food Chem. Toxicol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.talanta.2006.03.050","article-title":"Radical Scavenging Ability of Polyphenolic Compounds towards DPPH Free Radical","volume":"71","author":"Troncoso","year":"2007","journal-title":"Talanta"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Bourhia, M., Bouothmany, K., Bakrim, H., Hadrach, S., Salamatullah, A.M., Alzahrani, A., Khalil Alyahya, H., Albadr, N.A., Gmouh, S., and Laglaoui, A. (2021). Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds. Separations, 8.","DOI":"10.3390\/separations8080114"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Moussaoui, A.E., Bourhia, M., Jawhari, F.Z., Salamatullah, A.M., Ullah, R., Bari, A., Mahmood, H.M., Sohaib, M., Serhii, B., and Rozhenko, A. (2021). Chemical Profiling, Antioxidant, and Antimicrobial Activity against Drug-Resistant Microbes of Essential Oil from Withania frutescens L. Appl. Sci., 11.","DOI":"10.3390\/app11115168"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Lambert, R.J.W., Skandamis, P.N., Coote, P.J., and Nychas, G.J. (2001). A Study of the Minimum Inhibitory Concentration and Mode of Action of Oregano Essential Oil, Thymol and Carvacrol. J. Appl., 453\u2013462.","DOI":"10.1046\/j.1365-2672.2001.01428.x"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3590","DOI":"10.1021\/jf980154m","article-title":"Characterization of the Action of Selected Essential Oil Components on Gram-Negative Bacteria","volume":"46","author":"Helander","year":"1998","journal-title":"J. Agric. Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Jawhari, F.Z., Moussaoui, A.E., Bourhia, M., Imtara, H., Saghrouchni, H., Ammor, K., Ouassou, H., Elamine, Y., Ullah, R., and Ezzeldin, E. (2021). Anacyclus Pyrethrum Var. Pyrethrum (L.) and Anacyclus Pyrethrum Var. Depressus (Ball) Maire: Correlation between Total Phenolic and Flavonoid Contents with Antioxidant and Antimicrobial Activities of Chemically Characterized Extracts. Plants, 10.","DOI":"10.3390\/plants10010149"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1108133","DOI":"10.1155\/2021\/1108133","article-title":"Chemical Characterization and Antioxidant, Antimicrobial, and Insecticidal Properties of Essential Oil from Mentha pulegium L","volume":"2021","author":"Aimad","year":"2021","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"El Abdali, Y., Agour, A., Allali, A., Bourhia, M., El Moussaoui, A., Eloutassi, N., Salamatullah, A.M., Alzahrani, A., Ouahmane, L., and Aboul-Soud, M.A. (2022). Lavandula dentata L.: Phytochemical Analysis, Antioxidant, Antifungal and Insecticidal Activities of Its Essential Oil. Plants, 11.","DOI":"10.3390\/plants11030311"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zalegh, I., Akssira, M., Bourhia, M., Mellouki, F., Rhallabi, N., Salamatullah, A.M., Alkaltham, M.S., Khalil Alyahya, H., and Mhand, R.A. (2021). A Review on Cistus Sp.: Phytochemical and Antimicrobial Activities. Plants, 10.","DOI":"10.3390\/plants10061214"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"798259","DOI":"10.3389\/fpls.2022.798259","article-title":"Chemistry, Insecticidal and Repellent Activities of Essential Oils From Origanum Compactum Benth Used in the Mediterranean Diet","volume":"13","author":"Bourhia","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.mycmed.2017.04.008","article-title":"The Antifungal Activity of Moroccan Plants and the Mechanism of Action of Secondary Metabolites from Plants","volume":"27","author":"Lagrouh","year":"2017","journal-title":"J. De Mycol. Med."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/11\/3539\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:22:36Z","timestamp":1760138556000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/11\/3539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,31]]},"references-count":43,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["molecules27113539"],"URL":"https:\/\/doi.org\/10.3390\/molecules27113539","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,31]]}}}