{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T05:46:12Z","timestamp":1780465572435,"version":"3.54.1"},"reference-count":29,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["No. U20A2053"],"award-info":[{"award-number":["No. U20A2053"]}]},{"name":"National Natural Science Foundation of China","award":["No. 20200402050NC"],"award-info":[{"award-number":["No. 20200402050NC"]}]},{"name":"National Natural Science Foundation of China","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]},{"name":"Jilin Province Science and Technology Development Program","award":["No. U20A2053"],"award-info":[{"award-number":["No. U20A2053"]}]},{"name":"Jilin Province Science and Technology Development Program","award":["No. 20200402050NC"],"award-info":[{"award-number":["No. 20200402050NC"]}]},{"name":"Jilin Province Science and Technology Development Program","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["No. U20A2053"],"award-info":[{"award-number":["No. U20A2053"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["No. 20200402050NC"],"award-info":[{"award-number":["No. 20200402050NC"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Previous studies have shown that circular RNAs are directly or indirectly involved in the occurrence of various diseases by regulating gene expression. However, the acting mechanism of circular RNAs in endometritis remains unclear. In this study, we successfully established an endometritis model in mouse using Escherichia coli; endometrial integrity was destroyed, inflammatory cells infiltrated and the expression of IL-6, IL-1\u03b2, TNF-\u03b1 was significantly up-regulated. We analyzed and screened the circular RNA expression profiles between healthy and endometritis-stricken mice by the Illumina HiSeq platform, and used qRT-PCR method to verify the different expressions of circular RNAs. Gene ontology (GO) analysis showed that circular RNAs were mainly involved in biological processes such as the positive regulation of transcription from RNA polymerase POL II promoter and the negative regulation of cell proliferation. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of circular RNAs target genes may be involved in the TGF-\u03b2 signaling pathway. We verified the expression of TGF-\u03b2 and its related factors; the mRNA of TGF-\u03b21 and smad7 were significantly up-regulated in endometritis mouse (p &lt; 0.01) and the protein expression level of p-smad3 was significantly decreased (p &lt; 0.01). Finally, we constructed a circular RNAs\u2013miRNA network to elucidate the potential regulatory relationship between two small molecules. This research may provide new ideas for circular RNAs in the treatment of endometritis.<\/jats:p>","DOI":"10.3390\/molecules27123682","type":"journal-article","created":{"date-parts":[[2022,6,12]],"date-time":"2022-06-12T22:37:20Z","timestamp":1655073440000},"page":"3682","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Expression and Bioinformatics Analysis of Circular RNAs in Endometritis Mouse Uterus Tissues"],"prefix":"10.3390","volume":"27","author":[{"given":"Zhiqiang","family":"Li","sequence":"first","affiliation":[{"name":"Joint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Shi","sequence":"additional","affiliation":[{"name":"Joint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqing","family":"Li","sequence":"additional","affiliation":[{"name":"Joint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Joint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfa","family":"Lu","sequence":"additional","affiliation":[{"name":"Joint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Joint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2686","DOI":"10.3168\/jds.2018-15595","article-title":"A model of clinical endometritis in Holstein heifers using pathogenic Escherichia coli and Trueperella pyogenes","volume":"102","author":"Piersanti","year":"2019","journal-title":"J. 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