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The chemical profile of supercritical extracts of T. obliquus was analyzed by ultra-high-performance liquid chromatography\u2013high-resolution mass spectrometry with electrospray ionization (UHPLC-ESI-HRMS). Moreover, the impact of ScCO2 on the microbiological and metal profile of the biomass was monitored. The application of the pre-treatment increased the extraction yield approximately three-fold compared to the control. In the obtained extracts (control and pre-treated extracts), the identified components belonged to triacylglyceroles, fatty acid derivatives, diacylglycerophosphocholines and diacylglycerophosphoserines, pigments, terpenes, and steroids. Triacylglycerols (65%) were the most dominant group of compounds in the control extract. The pre-treatment decreased the percentage of triacylglycerols to 2%, while the abundance of fatty acid derivatives was significantly increased (82%). In addition, the pre-treatment led to an increase in the percentages of carotenoids, terpenoids, and steroids. Furthermore, it was determined that ScCO2 extraction reduced the number of microorganisms in the biomass. Considering its microbiological and metal profiles, the biomass after ScCO2 can potentially be used as a safe and important source of organic compounds.<\/jats:p>","DOI":"10.3390\/molecules27123883","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T05:25:11Z","timestamp":1655443511000},"page":"3883","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Supercritical CO2 Extract from Microalga Tetradesmus obliquus: The Effect of High-Pressure Pre-Treatment"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3891-7266","authenticated-orcid":false,"given":"Jelena","family":"Vladi\u0107","sequence":"first","affiliation":[{"name":"Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21102 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0727-6662","authenticated-orcid":false,"given":"Igor","family":"Jerkovi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Technology, University of Split, Ru\u0111era Bo\u0161kovi\u0107a 35, 21000 Split, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Radman","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Technology, University of Split, Ru\u0111era Bo\u0161kovi\u0107a 35, 21000 Split, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Molnar Jazi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovi\u0107a 3, 21102 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Ferreira","sequence":"additional","affiliation":[{"name":"LNEG, National Laboratory of Energy and Geology I.P., Bioenergy and Biorefineries Unit, Pa\u00e7o Lumiar 22, 1649-038 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5026-3365","authenticated-orcid":false,"given":"Sne\u017eana","family":"Maleti\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovi\u0107a 3, 21102 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0196-6360","authenticated-orcid":false,"given":"Luisa","family":"Gouveia","sequence":"additional","affiliation":[{"name":"LNEG, National Laboratory of Energy and Geology I.P., Bioenergy and Biorefineries Unit, Pa\u00e7o Lumiar 22, 1649-038 Lisbon, Portugal"},{"name":"GreenCoLab\u2014Green Ocean Technologies and Products Collaborative Laboratory, Centro de Ci\u00eancias do Mar do Algarve, Universidade do Algarve, Campus Gambelas, Edif\u00edcio 7, 8005-139 Faro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ferreira, A., Reis, A., Vidovic, S., Vladic, J., Gkelis, S., Melkonyan, L., Avetisova, G., Congestri, R., Aci\u00e9n, G., and Mu\u00f1oz, R. 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