{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:12:11Z","timestamp":1787242331347,"version":"build-2736575974"},"reference-count":44,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Croatian Science Foundation grant NeuroReact","award":["IP-2016-06-8636"],"award-info":[{"award-number":["IP-2016-06-8636"]}]},{"name":"Croatian Science Foundation grant NeuroReact","award":["PN-III-P2-2.1-PED-2019-0799"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2019-0799"]}]},{"name":"Croatian Science Foundation grant NeuroReact","award":["GA KK 01.1.1.01.0007"],"award-info":[{"award-number":["GA KK 01.1.1.01.0007"]}]},{"name":"Romanian National Authority for Scientific Research, UEFISCDI","award":["IP-2016-06-8636"],"award-info":[{"award-number":["IP-2016-06-8636"]}]},{"name":"Romanian National Authority for Scientific Research, UEFISCDI","award":["PN-III-P2-2.1-PED-2019-0799"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2019-0799"]}]},{"name":"Romanian National Authority for Scientific Research, UEFISCDI","award":["GA KK 01.1.1.01.0007"],"award-info":[{"award-number":["GA KK 01.1.1.01.0007"]}]},{"name":"Scientific Centre of Excellence for Basic, Clinical, and Translational Neuroscience","award":["IP-2016-06-8636"],"award-info":[{"award-number":["IP-2016-06-8636"]}]},{"name":"Scientific Centre of Excellence for Basic, Clinical, and Translational Neuroscience","award":["PN-III-P2-2.1-PED-2019-0799"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2019-0799"]}]},{"name":"Scientific Centre of Excellence for Basic, Clinical, and Translational Neuroscience","award":["GA KK 01.1.1.01.0007"],"award-info":[{"award-number":["GA KK 01.1.1.01.0007"]}]},{"name":"European Regional Development Fund","award":["IP-2016-06-8636"],"award-info":[{"award-number":["IP-2016-06-8636"]}]},{"name":"European Regional Development Fund","award":["PN-III-P2-2.1-PED-2019-0799"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2019-0799"]}]},{"name":"European Regional Development Fund","award":["GA KK 01.1.1.01.0007"],"award-info":[{"award-number":["GA KK 01.1.1.01.0007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In this study, we developed a high-resolution tandem mass spectrometry (HR MS) approach to assess presumed changes in gangliosidome of a human hippocampus affected by temporal lobe epilepsy (TLE) in comparison with a normal hippocampus. Gangliosides, membrane glycolipids, are particularly diverse and abundant in the human brain, and participate in ion transport and modulation of neuronal excitability. Changes in structural ganglioside pattern potentially linked to TLE molecular pathogenesis have not been explored in detail. Aiming to characterize TLE-specific gangliosidome, we analyzed the native gangliosides purified from a human hippocampal tissue sample affected by TLE and a control hippocampus using HR MS. Marked differences of ganglioside expression were shown in TLE vs. control, particularly with respect to the sialylation degree of components, discovered as a characteristic feature of TLE. Another major finding is the occurrence of tetrasialofucogangliosides in TLE and species modified by either O-acetylation or CH3COO\u2212. Structural analysis by higher-energy collisional dissociation (HCD) MS\/MS gave rise to fragmentation patterns implying that the GQ1b (d18:1\/18:0) isomer is specifically associated with TLE. Further investigation in a larger sample is needed in order to confirm the discovery of ganglioside structures specifically expressed in human TLE and to provide information on the probable role of gangliosides in the molecular events underlying seizures.<\/jats:p>","DOI":"10.3390\/molecules27134056","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T22:43:00Z","timestamp":1656024180000},"page":"4056","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry"],"prefix":"10.3390","volume":"27","author":[{"given":"Raluca","family":"Ica","sequence":"first","affiliation":[{"name":"Department of Condensed Matter, National Institute for Research and Development in Electrochemistry and Condensed Matter, 300224 Timisoara, Romania"},{"name":"Faculty of Physics, West University of Timisoara, 300223 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4309-5447","authenticated-orcid":false,"given":"Kristina","family":"Mlinac-Jerkovic","sequence":"additional","affiliation":[{"name":"Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8995-6396","authenticated-orcid":false,"given":"Katarina","family":"Ilic","sequence":"additional","affiliation":[{"name":"Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10000 Zagreb, Croatia"},{"name":"BRAIN Centre, Department of Neuroimaging, Institute of Psychiatry, Psychology and Neuroscience (IOPPN), King\u2019s College London, London SE5 9NU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomislav","family":"Sajko","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, University Hospital Sestre Milosrdnice, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2998-0458","authenticated-orcid":false,"given":"Cristian V. A.","family":"Munteanu","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry of the Romanian Academy, Splaiul Independen\u021bei 296, 060031 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina D.","family":"Zamfir","sequence":"additional","affiliation":[{"name":"Department of Condensed Matter, National Institute for Research and Development in Electrochemistry and Condensed Matter, 300224 Timisoara, Romania"},{"name":"Faculty of Physics, West University of Timisoara, 300223 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svjetlana","family":"Kalanj-Bognar","sequence":"additional","affiliation":[{"name":"Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/978-1-4684-7844-0_46","article-title":"Gangliosides and synaptic transmission","volume":"125","author":"Svennerholm","year":"1980","journal-title":"Adv. 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