{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:13:11Z","timestamp":1770747191133,"version":"3.49.0"},"reference-count":52,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,7,3]],"date-time":"2022-07-03T00:00:00Z","timestamp":1656806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["82002001"],"award-info":[{"award-number":["82002001"]}]},{"name":"National Natural Science Foundation of China","award":["S202110159041"],"award-info":[{"award-number":["S202110159041"]}]},{"name":"Innovation and Entrepreneurship Training Program of China Medical University","award":["82002001"],"award-info":[{"award-number":["82002001"]}]},{"name":"Innovation and Entrepreneurship Training Program of China Medical University","award":["S202110159041"],"award-info":[{"award-number":["S202110159041"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Chronic alcohol exposure can cause myocardial degenerative diseases, manifested as cardiac insufficiency, arrhythmia, etc. These are defined as alcoholic cardiomyopathy (ACM). Alcohol-mediated myocardial injury has previously been studied through metabolomics, and it has been proved to be involved in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway concerning unsaturated fatty acids biosynthesis and oxidative phosphorylation, which tentatively explored the mechanism of ACM induced by chronic drinking. To further study alcohol-induced myocardial injury, myocardial specimens from a previously successfully established mouse model of ACM were subjected to histological, echocardiographic, and proteomic analyses, and validated by real-time quantitative polymerase chain reaction (qPCR). Results of histopathology and echocardiography showed the hypertrophy of cardiomyocytes, the dilation of ventricles, and decreased cardiac function. Proteomic results, available via ProteomeXchange with identifier PXD032949, revealed 56 differentially expressed proteins (DEPs) were identified, which have the potential to be involved in the KEGG pathway related to fatty acid biosynthesis disorders, lipid metabolism disorders, oxidative stress, and, ultimately, in the development of dilated cardiomyopathy (DCM). The present study further elucidates the underlying effects of myocardial injury due to chronic alcohol intake, laying a foundation for further studies to clarify the potential mechanisms of ACM.<\/jats:p>","DOI":"10.3390\/molecules27134284","type":"journal-article","created":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T20:59:18Z","timestamp":1656968358000},"page":"4284","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Myocardial Injury Caused by Chronic Alcohol Exposure\u2014A Pilot Study Based on Proteomics"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0459-0680","authenticated-orcid":false,"given":"Xiaonan","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110122, China"},{"name":"The Third Clinical Department, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zihan","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110122, China"},{"name":"Liaoning Province Key Laboratory of Forensic Bio-Evidence Science, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongxuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110122, China"},{"name":"The Third Clinical Department, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Forensic Analytical Toxicology, School of Forensic Medicine, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honghui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Forensic Analytical Toxicology, School of Forensic Medicine, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiawei","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110122, China"},{"name":"Liaoning Province Key Laboratory of Forensic Bio-Evidence Science, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4325-2940","authenticated-orcid":false,"given":"Huaxin","family":"Sheng","sequence":"additional","affiliation":[{"name":"Multidisciplinary Neuroprotection Laboratories, Center of Perioperative Organ Protection, Department of Anesthesiology, Duke University Medical Center, Durham, NC 27710, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meihui","family":"Tian","sequence":"additional","affiliation":[{"name":"Liaoning Province Key Laboratory of Forensic Bio-Evidence Science, Shenyang 110122, China"},{"name":"Department of Forensic Genetics and Biology, School of Forensic Medicine, China Medical University, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4275-0788","authenticated-orcid":false,"given":"Zhipeng","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110122, China"},{"name":"Liaoning Province Key Laboratory of Forensic Bio-Evidence Science, Shenyang 110122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.neubiorev.2018.08.011","article-title":"Facets of impulsivity and alcohol use: What role do emotions play?","volume":"106","author":"Herman","year":"2019","journal-title":"Neurosci. 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