{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T19:31:05Z","timestamp":1785353465570,"version":"3.55.0"},"reference-count":37,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:00:00Z","timestamp":1658188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21505115"],"award-info":[{"award-number":["21505115"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of 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the Guizhou Provincial Education Department","award":["2021-2-31"],"award-info":[{"award-number":["2021-2-31"]}]},{"name":"Science and Technology for Youth Talent Growth Project of the Guizhou Provincial Education Department","award":["2021XYZD02"],"award-info":[{"award-number":["2021XYZD02"]}]},{"name":"Science and Technology for Youth Talent Growth Project of the Guizhou Provincial Education Department","award":["21XYXS05"],"award-info":[{"award-number":["21XYXS05"]}]},{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan","award":["21505115"],"award-info":[{"award-number":["21505115"]}]},{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan","award":["KY2018078"],"award-info":[{"award-number":["KY2018078"]}]},{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan","award":["KY2020217"],"award-info":[{"award-number":["KY2020217"]}]},{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan","award":["2021-2-31"],"award-info":[{"award-number":["2021-2-31"]}]},{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan","award":["2021XYZD02"],"award-info":[{"award-number":["2021XYZD02"]}]},{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan","award":["21XYXS05"],"award-info":[{"award-number":["21XYXS05"]}]},{"name":"Projects of Minzu Normal University of Xingyi","award":["21505115"],"award-info":[{"award-number":["21505115"]}]},{"name":"Projects of Minzu Normal University of Xingyi","award":["KY2018078"],"award-info":[{"award-number":["KY2018078"]}]},{"name":"Projects of Minzu Normal University of Xingyi","award":["KY2020217"],"award-info":[{"award-number":["KY2020217"]}]},{"name":"Projects of Minzu Normal University of Xingyi","award":["2021-2-31"],"award-info":[{"award-number":["2021-2-31"]}]},{"name":"Projects of Minzu 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In this study, composites of silver nanoparticles (AgNPs) wrapped with nitrogen-doped carbon quantum dots (N-CQDs) were synthesised utilising N-CQDs as the reducing and stabilising agents in a single reaction with AgNO3. The morphology and structure, absorption properties, functional groups, and fluorescence properties were characterised by transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, ultraviolet spectroscopy, fluorescence spectroscopy, and X-ray diffraction spectroscopy. In addition, we developed a novel method based on AgNPs\/N-CQDs for the detection of uric acid using the enzymatic reaction of uric acid oxidase. The fluorescence enhancement of the AgNPs\/N-CQDs composite was linear (R2 = 0.9971) in the range of 2.0\u201360 \u03bcmol\/L, and gave a detection limit of 0.53 \u03bcmol\/L. Trace uric acid was successfully determined in real serum samples from the serum of 10 healthy candidates and 10 gout patients, and the results were consistent with those recorded by Qianxinan Prefecture People\u2019s Hospital. These results indicate that the developed AgNP\/N-CQD system can provide a universal platform for detecting the multispecies ratio fluorescence of H2O2 generation in other biological systems.<\/jats:p>","DOI":"10.3390\/molecules27144586","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T05:37:53Z","timestamp":1658209073000},"page":"4586","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Silver Nanoparticle-Functionalised Nitrogen-Doped Carbon Quantum Dots for the Highly Efficient Determination of Uric Acid"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6199-3021","authenticated-orcid":false,"given":"Qianchun","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan, School of Biology and Chemistry, Minzu Normal University of Xingyi, Xingyi 562400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxin","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan, School of Biology and Chemistry, Minzu Normal University of Xingyi, Xingyi 562400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengling","family":"Tian","sequence":"additional","affiliation":[{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan, School of Biology and Chemistry, Minzu Normal University of Xingyi, Xingyi 562400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xixi","family":"Long","sequence":"additional","affiliation":[{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan, School of Biology and Chemistry, Minzu Normal University of Xingyi, Xingyi 562400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqi","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan, School of Biology and Chemistry, Minzu Normal University of Xingyi, Xingyi 562400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan, School of Biology and Chemistry, Minzu Normal University of Xingyi, Xingyi 562400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linchun","family":"Bao","sequence":"additional","affiliation":[{"name":"Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan, School of Biology and Chemistry, Minzu Normal University of Xingyi, Xingyi 562400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10829","DOI":"10.1021\/acs.jmedchem.0c00223","article-title":"Novel human urate transporter 1 inhibitors as hypouricemic drug candidates with favorable druggability","volume":"63","author":"Zhao","year":"2020","journal-title":"J. Med. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.bios.2016.06.017","article-title":"Rapid and highly-sensitive uric acid sensing based on enzymatic catalysis-induced upconversion inner filter effect","volume":"86","author":"Long","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s00604-018-3030-0","article-title":"Enzymatic determination of uric acid using water-soluble CuInS\/ZnS quantum dots as a fluorescent probe","volume":"185","author":"Zhang","year":"2018","journal-title":"Microchim. Acta"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/j.bios.2017.01.020","article-title":"Gold nanoclusters as switch-off fluorescent probe for detection of uric acid based on the inner filter effect of hydrogen peroxide-mediated enlargement of gold nanoparticles","volume":"91","author":"Liu","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.thromres.2016.06.020","article-title":"Serum uric acid, gout, and venous thromboembolism: The atherosclerosis risk in communities study","volume":"144","author":"Kubota","year":"2016","journal-title":"Thromb. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"122499","DOI":"10.1016\/j.talanta.2021.122499","article-title":"Simultaneous and sensitive detection of multiple small biological molecules by microfluidic paper-based analytical device integrated with zinc oxide nanorods","volume":"232","author":"Feng","year":"2021","journal-title":"Talanta"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jchromb.2015.09.025","article-title":"Graphitic carbon nitrides modifed hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry","volume":"1004","author":"Sun","year":"2015","journal-title":"J. Chromatogr. B"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1039\/c2ay26362a","article-title":"An eco-friendly hydrophilic interaction HPLC method for the determination of renal function biomarkers, creatinine and uric acid, in human fluids","volume":"5","author":"Zhou","year":"2013","journal-title":"Anal. Methods"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Minta, D., Moyseowicz, A., Gryglewicz, S., and Gryglewicz, G. (2020). A promising electrochemical platform for dopamine and uric acid detection based on a Polyaniline\/Iron Oxide-Tin Oxide\/Reduced Graphene Oxide ternary composite. Molecules, 25.","DOI":"10.3390\/molecules25245869"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.aca.2019.12.069","article-title":"A \u201cnaked-eye\u2019\u2019 colorimetric and ratiometric fluorescence probe for uric acid based on Ti3C2 MXene quantum dots","volume":"1103","author":"Liu","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10376","DOI":"10.1021\/acs.analchem.7b02344","article-title":"Nanomolar level detection of uric acid in blood serum and pest-infested grain samples by an amphiphilic probe","volume":"89","author":"Dey","year":"2017","journal-title":"Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5654","DOI":"10.1021\/acs.inorgchem.8b03629","article-title":"A pyrene-functionalized metal-organic framework for nonenzymatic and ratiometric detection of uric acid in biological fluid via conformational change","volume":"58","author":"Dalapati","year":"2019","journal-title":"Inorg. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.saa.2017.09.037","article-title":"Green synthesis of carbon dots from pork and application as nanosensors for uric acid detection","volume":"190","author":"Zhao","year":"2018","journal-title":"Spectrochim. Acta A"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1021\/ac503595y","article-title":"Nanosensor composed of nitrogen-doped carbon dots and gold nanoparticles for highly selective detection of cysteine with multiple signals","volume":"87","author":"Deng","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s00604-018-2814-6","article-title":"Colorimetric and fluorometric determination of uric acid based on the use of nitrogen-doped carbon quantum dots and silver triangular nanoprisms","volume":"185","author":"Wang","year":"2018","journal-title":"Microchim. Acta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3089","DOI":"10.1021\/acsbiomaterials.9b00394","article-title":"Amygdalin-functionalized carbon quantum dots for probing \u03b2-glucosidase activity for cancer diagnosis and therapeutics","volume":"5","author":"Kalaiyarasan","year":"2019","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4198","DOI":"10.1039\/C7CS00399D","article-title":"Carbon-based hybrid nanogels: A synergistic nanoplatform for combined biosensing, bioimaging, and responsive drug delivery","volume":"47","author":"Wang","year":"2018","journal-title":"Chem. Soc. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1039\/C5AY03208C","article-title":"A geen synthesis of highly fluorescent nitrogen-doped graphene quantum dots for the highly sensitive and selective detection of Mercury(II) ions and biothiols","volume":"8","author":"Yan","year":"2016","journal-title":"Anal. Methods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"42816","DOI":"10.1021\/acsami.7b14541","article-title":"Construction of plasmonic Ag and nitrogen-doped graphene quantum dots codecorated ultrathin graphitic carbon nitride nanosheet composites with enhanced photocatalytic activity: Full-spectrum response ability and mechanism insight","volume":"9","author":"Deng","year":"2017","journal-title":"ACS Appl. Mater. Inter."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6689","DOI":"10.1021\/ac501497d","article-title":"In situ growth of silver nanoparticles on graphene quantum dots for ultrasensitive colorimetric detection of H2O2 and glucose","volume":"86","author":"Chen","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/s00339-004-2600-7","article-title":"HAADF study of Au-Pt core-shell bimetallic nanoparticles","volume":"79","author":"Gutierrez","year":"2004","journal-title":"Appl. Phys. A"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.1007\/s00216-019-01697-2","article-title":"Silver nanoparticles capped with carbon dots as a fluorescent probe for the highly sensitive \u201coff\u2013on\u201d sensing of sulfide ions in water","volume":"411","author":"Sinduja","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/s00604-019-3361-5","article-title":"Green emitting carbon dots for sensitive fluorometric determination of cartap based on its aggregation effect on gold nanoparticles","volume":"186","author":"Yang","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"27259","DOI":"10.1021\/acsami.9b10176","article-title":"Ultrabright full color carbon dots by fine-tuning crystal morphology controllable synthesis for multicolor bioimaging and sensing","volume":"11","author":"Huo","year":"2019","journal-title":"ACS Appl. Mater. Inter."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.colsurfb.2012.12.033","article-title":"The synthesis of citrate-modified silver nanoparticles in an aqueous suspension of graphene oxide nanosheets and their antibacterial activity","volume":"105","author":"Das","year":"2013","journal-title":"Colloids Surf B Biointerfaces"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1039\/C0JM02806A","article-title":"Preparation, characterization and antibacterial properties of silver-modified graphene oxide","volume":"21","author":"Ma","year":"2011","journal-title":"J. Mater. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1039\/C9TB02934F","article-title":"Orange emission N doped carbon dots as fluorescent and colorimetric dual-mode probe for nitrite detection and cellular imaging","volume":"8","author":"Jia","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8503","DOI":"10.1039\/D0NJ01025A","article-title":"A green carbon dots-based fluorescent sensor for selective and visual detection of nitrite triggered by the nitrite-thiol reaction","volume":"44","author":"Yue","year":"2020","journal-title":"New J. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.jallcom.2014.04.051","article-title":"P-type conductivity and stability of Ag-N codoped ZnO thin films","volume":"609","author":"Li","year":"2014","journal-title":"J. Alloy. Compd."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"015005","DOI":"10.1088\/2050-6120\/ab5d8c","article-title":"One-step hydrothermal synthesis of silver-doped carbon quantum dots for highly selestive detection of uric acid","volume":"8","author":"Zhuo","year":"2020","journal-title":"Methods Appl. Fluoresc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"118076","DOI":"10.1016\/j.jlumin.2021.118076","article-title":"An enzyme-free fluorescent sensing platform for the detection of uric acid in human urine","volume":"236","author":"Zheng","year":"2021","journal-title":"J. Lumin."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s00604-021-04971-2","article-title":"A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples","volume":"188","author":"Li","year":"2021","journal-title":"Microchim. Acta"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1007\/s00604-021-04923-w","article-title":"AIEgens-based fluorescent covalent organic framework in construction of chemiluminescence resonance energy transfer system for serum uric acid detection","volume":"188","author":"Tan","year":"2021","journal-title":"Microchim. Acta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.talanta.2018.08.015","article-title":"A novel ratiometric fluorescent probe for the detection of uric acid in human blood based on H2O2-mediated fluorescence quenching of gold\/silver nanoclusters","volume":"191","author":"Wang","year":"2019","journal-title":"Talanta"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"9333","DOI":"10.1021\/acsomega.9b00874","article-title":"Nitrogen, cobalt Co-doped fluorescent magnetic carbon dots as ratiometric fluorescent probes for cholesterol and uric acid in human blood serum","volume":"4","author":"Huang","year":"2019","journal-title":"ACS Omega"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1007\/s00604-019-3862-2","article-title":"A dual-signal colorimetric and ratiometric fluorescent nanoprobe for enzymatic determination of uric acid by using silicon nanoparticles","volume":"186","author":"Wu","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"107797","DOI":"10.1016\/j.dyepig.2019.107797","article-title":"Fluorescent detection of uric acid through photoinduced electron transfer using luminol-terbium(III) nanoparticles synthesized via aggregation-induced fluorescence strategy","volume":"172","author":"Qi","year":"2020","journal-title":"Dye. Pigment."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/14\/4586\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:53:27Z","timestamp":1760140407000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/14\/4586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,19]]},"references-count":37,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["molecules27144586"],"URL":"https:\/\/doi.org\/10.3390\/molecules27144586","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,19]]}}}