{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:23:44Z","timestamp":1790501024943,"version":"4.1.0"},"reference-count":50,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T00:00:00Z","timestamp":1658361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agence Nationale de la Recherche","award":["ANR 2010 BLAN 7061"],"award-info":[{"award-number":["ANR 2010 BLAN 7061"]}]},{"name":"the University of Carthage (Tunisia)","award":["ANR 2010 BLAN 7061"],"award-info":[{"award-number":["ANR 2010 BLAN 7061"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>SuccFerr (N-[4-ferrocenyl,5-5-bis (4-hydroxyphenyl)-pent-4-enyl]-succinimide) has remarkable antiproliferative effects in vitro, attributed to the formation of a stabilized quinone methide. The present article reports in vivo results for a possible preclinical study. SuccFerr is lipophilic and insoluble in water, so the development of a formulation to obviate this inconvenience was necessary. This was achieved by complexation with randomly methylated cyclodextrins (RAME\u00dfCDs). This supramolecular water-soluble system allowed the in vivo experiments below to proceed. Application of SuccFerr on the glioblastoma cancer cell line U87 indicates that it affects the cellular cycle by inducing a blockade at G0\/G1 phase, linked to apoptosis, and another one at the S phase, associated with senescence. Using healthy Fischer rats, we show that both intravenous and subcutaneous SuccFerr: RAME\u00dfCD administration at 5 mg\/kg lacks toxic effects on several organs. To reach lethality, doses higher than 200 mg\/kg need to be administered. These results prompted us to perform an ectopic in vivo study at 1 mg\/kg i.v. ferrocidiphenol SuccFerr using F98 cells xenografted in rats. Halting of cancer progression was observed after six days of injection, associated with an immunological defense response linked to the active principle. These results demonstrate that the properties of the selected ferrocidiphenol SuccFerr transfer successfully to in vivo conditions, leading to interesting therapeutic perspectives based on this chemistry.<\/jats:p>","DOI":"10.3390\/molecules27144651","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T22:38:50Z","timestamp":1658443130000},"page":"4651","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Succinimido\u2013Ferrocidiphenol Complexed with Cyclodextrins Inhibits Glioblastoma Tumor Growth In Vitro and In Vivo without Noticeable Adverse Toxicity"],"prefix":"10.3390","volume":"27","author":[{"given":"Feten","family":"Najlaoui","sequence":"first","affiliation":[{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques LR11IPT08, Institut Pasteur de Tunis, 13, Place Pasteur, Tunis 1002, Tunisia"},{"name":"Institute for Advanced Biosciences, INSERM U1209, CNRS UMR 5309, Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Research Unit of Plant Protection and Environment, Olive Tree Institute, BP 208, Tunis 1082, Tunisia"},{"name":"EA 3452\/CITHEFOR, Universit\u00e9 de Lorraine, 9 Avenue de la For\u00eat de Haye-BP 20199, CEDEX, F-54505 Vandoeuvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9425-1577","authenticated-orcid":false,"given":"Benoit","family":"Busser","sequence":"additional","affiliation":[{"name":"Institute for Advanced Biosciences, INSERM U1209, CNRS UMR 5309, Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7771-0268","authenticated-orcid":false,"given":"Germain Sotoing","family":"Ta\u00efwe","sequence":"additional","affiliation":[{"name":"Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, Buea P.O. Box 63, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5374-5346","authenticated-orcid":false,"given":"Pascal","family":"Pigeon","sequence":"additional","affiliation":[{"name":"Chimie ParisTech, 11 Rue Pierre et Marie Curie, CEDEX 05, F-75231 Paris, France"},{"name":"Institut Parisien de Chimie Mol\u00e9culaire (IPCM)\u2013UMR 8232, Sorbonne Universit\u00e9, 4 Place Jussieu, CEDEX 05, F-75252 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Sturm","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biology and Pathology, University Hospital of Grenoble, CS 10217, F-38043 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Giovannini","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biology and Pathology, University Hospital of Grenoble, CS 10217, F-38043 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naziha","family":"Marrakchi","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques LR11IPT08, Institut Pasteur de Tunis, 13, Place Pasteur, Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Rhouma","sequence":"additional","affiliation":[{"name":"Research Unit of Plant Protection and Environment, Olive Tree Institute, BP 208, Tunis 1082, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rard","family":"Jaouen","sequence":"additional","affiliation":[{"name":"Chimie ParisTech, 11 Rue Pierre et Marie Curie, CEDEX 05, F-75231 Paris, France"},{"name":"Institut Parisien de Chimie Mol\u00e9culaire (IPCM)\u2013UMR 8232, Sorbonne Universit\u00e9, 4 Place Jussieu, CEDEX 05, F-75252 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3594-0601","authenticated-orcid":false,"given":"St\u00e9phane","family":"Gibaud","sequence":"additional","affiliation":[{"name":"EA 3452\/CITHEFOR, Universit\u00e9 de Lorraine, 9 Avenue de la For\u00eat de Haye-BP 20199, CEDEX, F-54505 Vandoeuvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2782-9615","authenticated-orcid":false,"given":"Michel","family":"De Waard","sequence":"additional","affiliation":[{"name":"L\u2019institut du Thorax, CNRS, INSERM, Nantes Universit\u00e9, F-44000 Nantes, France"},{"name":"LabEx Ion Channels, Science and Therapeutics, F-06560 Valbonne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jaouen, G. 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