{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:31:18Z","timestamp":1791847878335,"version":"4.3.4"},"reference-count":52,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T00:00:00Z","timestamp":1658534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Startup Foundation of Beijing Institute of Technology","award":["3160022211905"],"award-info":[{"award-number":["3160022211905"]}]},{"name":"Startup Foundation of Beijing Institute of Technology","award":["3160011182007"],"award-info":[{"award-number":["3160011182007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Facile construction of functional nanomaterials with laccase-like activity is important in sustainable chemistry since laccase is featured as an efficient and promising catalyst especially for phenolic degradation but still has the challenges of high cost, low activity, poor stability and unsatisfied recyclability. In this paper, we report a simple method to synthesize nanozymes with enhanced laccase-like activity by the self-assembly of copper ions with various imidazole derivatives. In the case of 1-methylimidazole as the ligand, the as-synthesized nanozyme (denoted as Cu-MIM) has the highest yield and best activity among the nanozymes prepared. Compared to laccase, the Km of Cu-MIM nanozyme to phenol is much lower, and the vmax is 6.8 times higher. In addition, Cu-MIM maintains excellent stability in a variety of harsh environments, such as high pH, high temperature, high salt concentration, organic solvents and long-term storage. Based on the Cu-MIM nanozyme, we established a method for quantitatively detecting phenol concentration through a smartphone, which is believed to have important applications in environmental protection, pollutant detection and other fields.<\/jats:p>","DOI":"10.3390\/molecules27154712","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T01:42:13Z","timestamp":1658713333000},"page":"4712","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Facile Fabrication of 1-Methylimidazole\/Cu Nanozyme with Enhanced Laccase Activity for Fast Degradation and Sensitive Detection of Phenol Compounds"],"prefix":"10.3390","volume":"27","author":[{"given":"Yu","family":"Lei","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujun","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4100-9146","authenticated-orcid":false,"given":"Xinyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, China"},{"name":"Advanced Research Institute of Multidisciplinary Science, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1021\/ar000058i","article-title":"The Depth of Chemical Time and the Power of Enzymes as Catalysts","volume":"34","author":"Wolfenden","year":"2001","journal-title":"Acc. 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