{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T12:33:12Z","timestamp":1769171592977,"version":"3.49.0"},"reference-count":41,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T00:00:00Z","timestamp":1660262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1801000"],"award-info":[{"award-number":["2021YFD1801000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["20200901028SF"],"award-info":[{"award-number":["20200901028SF"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific and Technological Development Program Funded Project of Jilin Province","award":["2021YFD1801000"],"award-info":[{"award-number":["2021YFD1801000"]}]},{"name":"Scientific and Technological Development Program Funded Project of Jilin Province","award":["20200901028SF"],"award-info":[{"award-number":["20200901028SF"]}]},{"name":"Thousand Young Talents Program of the Chinese government (JZQ)","award":["2021YFD1801000"],"award-info":[{"award-number":["2021YFD1801000"]}]},{"name":"Thousand Young Talents Program of the Chinese government (JZQ)","award":["20200901028SF"],"award-info":[{"award-number":["20200901028SF"]}]},{"name":"startup fund from Jilin University (JZQ)","award":["2021YFD1801000"],"award-info":[{"award-number":["2021YFD1801000"]}]},{"name":"startup fund from Jilin University (JZQ)","award":["20200901028SF"],"award-info":[{"award-number":["20200901028SF"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Clostridium perfringens (C. perfringens) is an important foodborne pathogen that can cause diseases such as gas gangrene and necrotizing enteritis in a variety of economic animals, seriously affecting public health and the economic benefits and healthy development of the livestock and poultry breeding industry. Perfringolysin O (PFO) is an important virulence factor of C. perfringens and plays critical roles in necrotic enteritis and gas gangrene, rendering it an ideal target for developing new drugs against infections caused by this pathogen. In this study, based on biological activity inhibition assays, oligomerization tests and computational biology assays, we found that the foodborne natural component piceatannol reduced pore-forming activity with an inhibitory ratio of 83.84% in the concentration of 16 \u00b5g\/mL (IC50 = 7.83 \u00b5g\/mL) by binding with PFO directly and changing some of its secondary structures, including 3-Helix, A-helix, bend, and in turn, ultimately affecting oligomer formation. Furthermore, we confirmed that piceatannol protected human intestinal epithelial cells from the damage induced by PFO with LDH release reduced by 38.44% at 16 \u00b5g\/mL, based on a cytotoxicity test. By performing an animal experiment, we found the C. perfringens clones showed an approximate 10-fold reduction in infected mice. These results suggest that piceatannol may be a candidate for anti-C. perfringens drug development.<\/jats:p>","DOI":"10.3390\/molecules27165145","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T03:33:20Z","timestamp":1660534400000},"page":"5145","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Piceatannol Alleviates Clostridium perfringens Virulence by Inhibiting Perfringolysin O"],"prefix":"10.3390","volume":"27","author":[{"given":"Guizhen","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China"},{"name":"Measurement Biotechnique Research Center, College of Biological and Food Engineering, Jilin Engineering Normal University, Changchun 130052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongtao","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yawen","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodi","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuming","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8311-0894","authenticated-orcid":false,"given":"Jianfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haihua","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2800-5589","authenticated-orcid":false,"given":"Zhimin","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Hospital, Jilin University, Changchun 130021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiazhang","family":"Qiu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102287","DOI":"10.1016\/j.anaerobe.2020.102287","article-title":"Gas gangrene-associated gliding motility is regulated by the Clostridium perfringens CpAL\/VirSR system","volume":"66","author":"Valeriani","year":"2020","journal-title":"Anaerobe"},{"key":"ref_2","first-page":"141","article-title":"An update on the human and animal enteric pathogen Clostridium perfringens","volume":"7","author":"Raymond","year":"2018","journal-title":"Emerg. 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