{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T04:07:53Z","timestamp":1773029273612,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,13]],"date-time":"2022-08-13T00:00:00Z","timestamp":1660348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Foundation of Education Bureau of Hunan Province","award":["18A141"],"award-info":[{"award-number":["18A141"]}]},{"name":"Research Foundation of Education Bureau of Hunan Province","award":["SKLBNPC19250"],"award-info":[{"award-number":["SKLBNPC19250"]}]},{"name":"State Key Laboratory of Bio-organic and Natural Product Chemistry","award":["18A141"],"award-info":[{"award-number":["18A141"]}]},{"name":"State Key Laboratory of Bio-organic and Natural Product Chemistry","award":["SKLBNPC19250"],"award-info":[{"award-number":["SKLBNPC19250"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Nitrite (NO2\u2212) is a common pollutant and is widely present in the environment and in human bodies. The development of a rapid and accurate method for NO2\u2212 detection is always a very important task. Herein, we synthesized a partnered excited-state intermolecular proton transfer (ESIPT) fluorophore using the \u201cmulti-component one pot\u201d method, and used this as a probe (ESIPT-F) for sensing NO2\u2212. ESIPT-F exhibited bimodal emission in different solvents because of the solvent-mediated ESIPT reaction. The addition of NO2\u2212 caused an obvious change in colors and tautomeric fluorescence due to the graft of NO2\u2212 into the ESIPT-F molecules. From this basis, highly sensitive and selective analysis of NO2\u2212 was developed using tautomeric emission signaling, achieving sensitive detection of NO2\u2212 in the concentration range of 0~45 mM with a detection limit of 12.5 nM. More importantly, ESIPT-F showed the ability to anchor proteins and resulted in a recognition-driven \u201con-off\u201d ESIPT process, enabling it to become a powerful tool for fluorescence imaging of proteins or protein-based subcellular organelles. MTT experimental results revealed that ESIPT-F is low cytotoxic and has good membrane permeability to cells. Thus, ESIPT-F was further employed to image the tunneling nanotube in vitro HEC-1A cells, displaying high-resolution performance.<\/jats:p>","DOI":"10.3390\/molecules27165164","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T03:33:20Z","timestamp":1660534400000},"page":"5164","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Partnered Excited-State Intermolecular Proton Transfer Fluorescence (P-ESIPT) Signaling for Nitrate Sensing and High-Resolution Cell-Imaging"],"prefix":"10.3390","volume":"27","author":[{"given":"Pan","family":"Ma","sequence":"first","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuchun","family":"Gong","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanming","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"You","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingzhi","family":"He","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaoyun","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1017\/S0954422417000105","article-title":"The role of inorganic nitrate and nitrite in CVD","volume":"30","author":"Jackson","year":"2017","journal-title":"Nutr. 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