{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T04:18:38Z","timestamp":1786594718179,"version":"build-2736575974"},"reference-count":96,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T00:00:00Z","timestamp":1660608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hangzhou Medical and Health Science and Technology Project","award":["A20220302"],"award-info":[{"award-number":["A20220302"]}]},{"name":"Hangzhou Medical and Health Science and Technology Project","award":["20181228Y28"],"award-info":[{"award-number":["20181228Y28"]}]},{"name":"Hangzhou Foundation for Development of Science and Technology","award":["A20220302"],"award-info":[{"award-number":["A20220302"]}]},{"name":"Hangzhou Foundation for Development of Science and Technology","award":["20181228Y28"],"award-info":[{"award-number":["20181228Y28"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The abuse of buprenorphine and methadone has grown into a rising worldwide issue. After their consumption, buprenorphine, methadone and their metabolites can be found in the human organism. Due to the difficulty in the assessment of these compounds by routine drug screening, the importance of developing highly sensitive analytical approaches is undeniable. Liquid chromatography tandem mass spectrometry is the preferable technique for the determination of buprenorphine, methadone and their metabolites in biological matrices including urine, plasma, nails or oral fluids. This research aims to review a critical discussion of the latest trends for the monitoring of buprenorphine, methadone and their metabolites in various biological specimens.<\/jats:p>","DOI":"10.3390\/molecules27165211","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T23:44:03Z","timestamp":1660607043000},"page":"5211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analytical Approaches for the Determination of Buprenorphine, Methadone and Their Metabolites in Biological Matrices"],"prefix":"10.3390","volume":"27","author":[{"given":"Xiaoyue","family":"Shan","sequence":"first","affiliation":[{"name":"Hangzhou Occupational Disease Prevention and Treatment Hospital, Hangzhou 310014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengjian","family":"Cao","sequence":"additional","affiliation":[{"name":"Hangzhou Occupational Disease Prevention and Treatment Hospital, Hangzhou 310014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingsheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Hangzhou Occupational Disease Prevention and Treatment Hospital, Hangzhou 310014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"116006","DOI":"10.1016\/j.trac.2020.116006","article-title":"Recent biosensing advances in the rapid detection of illicit drugs","volume":"131","author":"Ahmed","year":"2020","journal-title":"TrAC Trend Anal. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.addbeh.2017.07.016","article-title":"Frequency and correlates of sleep disturbance in methadone and buprenorphine-maintained patients","volume":"76","author":"Dunn","year":"2018","journal-title":"Addict. Behav."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1038\/s41572-019-0137-5","article-title":"Opioid use disorder","volume":"6","author":"Strang","year":"2020","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_4","unstructured":"GBD 2016 Disease and Injury Incidence and Prevalence Collaborators (2017). Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990\u20132016: A systematic analysis for the Global Burden of Disease Study 2016. Lancet, 390, 1211\u20131259."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1016\/S0140-6736(18)33078-2","article-title":"Management of opioid use disorder in the USA: Present status and future directions","volume":"393","author":"Blanco","year":"2019","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1111\/bcp.13818","article-title":"A clinical trial on the acute effects of methadone and buprenorphine on actual driving and cognitive function of healthy volunteers","volume":"85","author":"Strand","year":"2018","journal-title":"Br. J. Clin. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1002\/dta.3104","article-title":"Determination of buprenorphine, norbuprenorphine, naloxone, and their glucuronides in urine by liquid chromatography-tandem mass spectrometry","volume":"13","author":"Mariottini","year":"2021","journal-title":"Drug Test. Anal."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.jpba.2010.09.023","article-title":"Development and validation of a highly sensitive GC\/MS method for the determination of buprenorphine and nor-buprenorphine in blood","volume":"54","author":"Papoutsis","year":"2011","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.jchromb.2006.11.025","article-title":"Simultaneous determination of buprenorphine, norbuprenorphine and the enantiomers of methadone and its metabolite (EDDP) in human plasma by liquid chromatography\/mass spectrometry","volume":"850","author":"Lofwall","year":"2007","journal-title":"J. Chromatogr. B"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1080\/10550887.2011.642758","article-title":"Effect of buprenorphine dose on treatment outcome","volume":"31","year":"2012","journal-title":"J. Addict. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.forsciint.2012.03.016","article-title":"Toxicological and pathological findings in a series of buprenorphine related deaths. Possible risk factors for fatal outcome","volume":"220","author":"Ahlner","year":"2012","journal-title":"Forensic Sci. Int."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1093\/jat\/bkv003","article-title":"Determination of buprenorphine, norbuprenorphine and naloxone in fingernail clippings and urine of patients under opioid substitution therapy","volume":"39","author":"Tzatzarakis","year":"2015","journal-title":"J. Anal. Toxicol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e3785","DOI":"10.1002\/bmc.3785","article-title":"Quantitative determination of buprenorphine, naloxone and their metabolites in rat plasma using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry","volume":"31","author":"Joshi","year":"2017","journal-title":"Biomed. Chromatogr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S1754-3207(08)60017-6","article-title":"Basic pharmacology of buprenorphine","volume":"1","author":"Ohtani","year":"2007","journal-title":"Eur. J. Pain Suppl."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"122755","DOI":"10.1016\/j.jchromb.2021.122755","article-title":"Comparison of liquid chromatography and supercritical fluid chromatography coupled to mass spectrometry","volume":"1177","author":"Mueller","year":"2021","journal-title":"J. Chromatogr. B Analyt. Technol. Biomed. Life. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1097\/FTD.0000000000000693","article-title":"Monitoring prenatal exposure to buprenorphine and methadone","volume":"42","author":"Tittarelli","year":"2020","journal-title":"Ther. Drug Monit."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1007\/s00216-019-01654-z","article-title":"Determination of methadone and EDDP in oral fluid using the dried saliva spots sampling approach and gas chromatography-tandem mass spectrometry","volume":"411","author":"Ribeiro","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1111\/1556-4029.14674","article-title":"Quantification of methadone and its metabolites: EDDP and EMDP determined in autopsy cases using LC-MS\/MS","volume":"66","author":"Nowak","year":"2021","journal-title":"J. Forensic Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1007\/s10337-018-3528-y","article-title":"Switchable hydrophilicity solvent-based homogenous liquid\u2013liquid microextraction (SHS-HLLME) combined with GC-FID for the quantification of methadone and tramadol","volume":"81","author":"Ahmar","year":"2018","journal-title":"Chromatographia"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3266","DOI":"10.1002\/jssc.202000078","article-title":"Effervescent tablet-assisted demulsified dispersive liquid\u2013liquid microextraction based on solidification of floating organic droplet for determination of methadone in water and biological samples prior to GC-flame ionization and GC-MS","volume":"43","author":"Jafarinejad","year":"2020","journal-title":"J. Sep. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1007\/s10337-020-03952-6","article-title":"Ultrasonic-air-assisted solidification of settled organic drop microextraction using terpene-based deep eutectic solvents for the effectual enrichment of methadone in biological samples","volume":"83","author":"Nedaei","year":"2020","journal-title":"Chromatographia"},{"key":"ref_22","first-page":"105","article-title":"Comparison of methadone level measurement by enzyme immunoassay with gas chromatography-mass spectrometry","volume":"9","author":"Forouzesh","year":"2019","journal-title":"J. Adv. Pharm. Educ. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Mu\u00f1oz-Mu\u00f1oz, A.C., Pekol, T., Awad, A., Hackett, P., Sullivan, L., Rodrigues, A., and Andrade, L. (2021). Norbuprenorphine interferences in urine drug testing LC\u2013MS\/MS confirmation methods from quetiapine metabolites. J. Anal. Toxicol., bkab113.","DOI":"10.1093\/jat\/bkab113"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"461241","DOI":"10.1016\/j.chroma.2020.461241","article-title":"Miniaturized extraction method for analysis of synthetic opioids in urine by microextraction with packed sorbent and liquid chromatography-tandem mass spectrometry","volume":"1624","author":"Da","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1373\/jalm.2018.027342","article-title":"A rapid dilute-and-shoot UPLC-MS\/MS assay to simultaneously measure 37 drugs and related metabolites in human urine for use in clinical pain management","volume":"3","author":"Dahlin","year":"2019","journal-title":"J. Appl. Lab. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.jpba.2019.01.014","article-title":"Rapid, hydrolysis-free, dilute-and-shoot method for the determination of buprenorphine, norbuprenorphine and their glucuronides in urine samples using UHPLC-MS\/MS","volume":"166","author":"Agostini","year":"2019","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.chroma.2018.05.019","article-title":"Development of an in-house mixed-mode solid-phase extraction for the determination of 16 basic drugs in urine by High Performance Liquid Chromatography-Ion Trap Mass Spectrometry","volume":"1560","author":"Musile","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1080\/08897077.2017.1356796","article-title":"Quantitative testing of buprenorphine and norbuprenorphine to identify urine sample spiking during office-based opioid treatment","volume":"38","author":"Suzuki","year":"2017","journal-title":"Subst. Abus."},{"key":"ref_29","first-page":"56","article-title":"Anion-doped overoxidized polypyrrole\/multiwalled carbon nanotubes modified glassy carbon electrode as a new electrochemical sensing platform for buprenorphine opioid drug","volume":"8","author":"Boldaji","year":"2021","journal-title":"Iran. J. Anal. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"130284","DOI":"10.1016\/j.snb.2021.130284","article-title":"Flexible and highly sensitive methadone sensor based on gold nanoparticles\/polythiophene modified carbon cloth platform","volume":"344","author":"Khorablou","year":"2021","journal-title":"Sens. Actuators B"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"38532","DOI":"10.1039\/D0RA06480G","article-title":"Simultaneous determination of methadone and morphine at a modified electrode with 3D b-MnO2 nanoflowers: Application for pharmaceutical sample analysis","volume":"10","author":"Akbari","year":"2020","journal-title":"RSC Adv."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"137508","DOI":"10.1149\/1945-7111\/abbb0d","article-title":"Electrochemical determination of naloxone using molecularly imprinted poly(para-phenylenediamine) sensor","volume":"167","author":"Shaabani","year":"2020","journal-title":"J. Electrochem. Soc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2909","DOI":"10.1007\/s13738-020-01962-7","article-title":"New and sensitive magnetic carbon paste electrode for voltammetry determination of morphine and methadone","volume":"17","author":"Yousefi","year":"2020","journal-title":"J. Iran. Chem. Soc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1007\/s00604-019-3736-7","article-title":"Highly selective extraction and voltammetric determination of the opioid drug buprenorphine via a carbon paste electrode impregnated with nano-sized molecularly imprinted polymer","volume":"186","author":"Alizadeh","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_35","first-page":"689","article-title":"Voltammetric Determination of methadone at stacked cysteic acid film and gold nanoparticles composite modified glassy carbon electrode","volume":"9","year":"2017","journal-title":"Anal. Bioanal. Electrochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.msec.2017.03.070","article-title":"An enhanced electrochemiluminescence sensor modified with a Ru(bpy)3(2+)\/Yb2O3 nanoparticle\/nafion composite for the analysis of methadone samples","volume":"76","author":"Hosseini","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e3926","DOI":"10.1002\/bmc.3926","article-title":"Solid-phase microextraction of methadone in urine samples by electrochemically co-deposited sol-gel\/Cu nanocomposite fiber","volume":"31","author":"Mohammadiazar","year":"2017","journal-title":"Biomed. Chromatogr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5210","DOI":"10.1039\/D0AY01374A","article-title":"An innovative highly-sensitive sensor based on carbon quantum dots and multiwall carbon nanotubes for electrochemical purposes and determination of methadone hydrochloride in real samples","volume":"12","author":"Rezaei","year":"2020","journal-title":"Anal. Methods"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"31530","DOI":"10.32598\/ijmtfm.v11i1.31530","article-title":"Research Paper: Determination of methadone and tramadol in vitreous humor specimens using dispersive liquid liquid microextraction and ultra high performance liquid chromatography","volume":"11","author":"Akhgari","year":"2021","journal-title":"Int. J. Med. Toxicol. Forensic Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3045","DOI":"10.1002\/jssc.202000221","article-title":"Dispersive solid-phase extraction of buprenorphine from biological fluids using metal-organic frameworks and its determination by ultra-performance liquid chromatography","volume":"43","author":"Mohammadi","year":"2020","journal-title":"J. Sep. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.microc.2018.12.065","article-title":"Bio-dispersive liquid liquid microextraction based on nano rhamnolipid aggregates combined with molecularly imprinted-solid phase extraction for selective determination of paracetamol in human urine samples followed by HPLC","volume":"146","author":"Abbasi","year":"2019","journal-title":"Microchem. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5214","DOI":"10.1039\/C8AY01757C","article-title":"Computational design, synthesis and utilization of a magnetic molecularly imprinted polymer on graphene oxide nanosheets for highly selective extraction and determination of buprenorphine in biological fluids and tablets","volume":"10","author":"Ganjavi","year":"2018","journal-title":"Anal. Methods"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3175","DOI":"10.1002\/jssc.201700213","article-title":"Computer-aided design and synthesis of a highly selective molecularly imprinted polymer for the extraction and determination of buprenorphine in biological fluids","volume":"40","author":"Ganjavi","year":"2017","journal-title":"J. Sep. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/s00604-020-04237-3","article-title":"Layered double hydroxide intercalated with tyrosine for ultrasonic-assisted microextraction of tramadol and methadone from biological samples followed by GC\/MS analysis","volume":"187","author":"Adlnasab","year":"2020","journal-title":"Microchim. Acta."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6113","DOI":"10.1007\/s00216-017-0547-8","article-title":"Dispersion of magnetic graphene oxide nanoparticles coated with a deep eutectic solvent using ultrasound assistance for preconcentration of methadone in biological and water samples followed by GC\u2013FID and GC\u2013MS","volume":"409","author":"Lamei","year":"2017","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1002\/chir.23008","article-title":"Simultaneous chiral separation of tramadol and methadone in tablets, human urine, and plasma by capillary electrophoresis using maltodextrin as the chiral selector","volume":"30","author":"Naghdi","year":"2018","journal-title":"Chirality"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1002\/jssc.202100095","article-title":"Solid-phase microextraction of methadone by using a chitosan nanocomposite incorporated with Polyoxomolibdate nanocluster\/Graphene oxide","volume":"44","author":"Abedi","year":"2021","journal-title":"J. Sep. Sci."},{"key":"ref_48","first-page":"580","article-title":"Optimization of New Sample preparation technique for the determination of methadone and codeine in plasma sample by GC-FID","volume":"31","author":"Jamil","year":"2020","journal-title":"J. Braz. Chem. Soc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1055\/s-0040-1701505","article-title":"Stereoselective analysis of methadone and EDDP in laboring women and neonates in plasma and dried blood spots and association with neonatal abstinence syndrome","volume":"38","author":"Metzger","year":"2021","journal-title":"Am. J. Perinat."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Tikhomirov, M., Po\u017aniak, B., and \u015aniegocki, T. (2021). High-performance liquid chromatography-tandem mass spectrometry for buprenorphine evaluation in plasma\u2014Application to pharmacokinetic studies in rabbits. Molecules, 26.","DOI":"10.3390\/molecules26020437"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"165","DOI":"10.4155\/bio-2018-0134","article-title":"Simultaneous quantification of high-dose naloxone and naloxone-3-\u03b2-d -glucuronide in human plasma by UHPLC\u2013MS\/MS","volume":"11","author":"Kongstad","year":"2019","journal-title":"Bioanalysis"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.jpba.2015.12.008","article-title":"Simultaneous determination of buprenorphine, norbuprenorphine and naloxone in human plasma by liquid chromatography\/tandem mass spectrometry","volume":"120","author":"Liu","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1002\/elps.201900044","article-title":"Enantioselective capillary electrophoresis for pharmacokinetic analysis of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in equines anesthetized with ketamine and isoflurane","volume":"40","author":"Theurillat","year":"2019","journal-title":"Electrophoresis"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s13738-017-1257-z","article-title":"Highly sensitive simultaneous quantification of buprenorphine and norbuprenorphine in human plasma by magnetic solid-phase extraction based on PpPDA\/Fe3O4 nanocomposite and high-performance liquid chromatography","volume":"15","author":"Gomar","year":"2018","journal-title":"J. Iran. Chem. Soc."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.ultsonch.2018.09.019","article-title":"Ultrasonically formation of supramolecular based ultrasound energy assisted solidification of floating organic drop microextraction for preconcentration of methadone in human plasma and saliva samples prior to gas chromatography\u2014Mass spectrometry","volume":"50","author":"Ezoddin","year":"2019","journal-title":"Ultrason. Sonochem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"9104","DOI":"10.1002\/rcm.9104","article-title":"Development and validation of a rapid liquid chromatography\/tandem mass spectrometry method to quantitate gabapentin and buprenorphine in human serum","volume":"35","author":"Phillips","year":"2021","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s11244-021-01483-4","article-title":"Introducing an electrochemical sensor based on two layers of Ag nanoparticles decorated graphene for rapid determination of methadone in human blood serum","volume":"65","author":"Baghayeri","year":"2022","journal-title":"Top. Catal."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.msec.2018.12.019","article-title":"In situ synthesis of rhodium nanoparticles\u2014Mesoporous carbon hybrid via a novel and facile nanocasting method for simultaneous determination of morphine and buprenorphine","volume":"97","author":"Jahanbakhshi","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1248\/bpb.b17-00691","article-title":"A novel method for determination of methadone in the serum by high-performance liquid chromatography with electrochemical detection","volume":"41","author":"Kokubun","year":"2018","journal-title":"Biol. Pharm. Bull."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1093\/jat\/bkaa032","article-title":"A UHPLC-MS-MS method for the determination of 84 drugs of abuse and pharmaceuticals in blood","volume":"45","author":"Orfanidis","year":"2021","journal-title":"J. Anal. Toxicol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"122226","DOI":"10.1016\/j.jchromb.2020.122226","article-title":"Quantification of methadone, buprenorphine, naloxone, opioids, and their derivates in whole blood by liquid chromatography-high-resolution mass spectrometry: Analysis of their involvement in fatal forensic cases","volume":"1152","author":"Feliu","year":"2020","journal-title":"J. Chromatogr. B"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1097\/FTD.0000000000000674","article-title":"A sensitive LC-MS\/MS assay for the quantification of methadone and its metabolites in dried blood spots: Comparison with plasma","volume":"42","author":"Davari","year":"2020","journal-title":"Ther. Drug Monit."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1002\/elan.202100266","article-title":"Determination of buprenorphine (BUP) with molecularly imprinted polymer Zn\/La3+ metal organic framework on modified glassy carbon electrode (GCE)","volume":"34","author":"Yahyapour","year":"2022","journal-title":"Electroanalysis"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1093\/jat\/bkx099","article-title":"Quantification of Methadone and Main Metabolites in Nails","volume":"42","author":"Magalhaes","year":"2018","journal-title":"J. Anal. Toxicol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"105843","DOI":"10.1016\/j.microc.2020.105843","article-title":"Polyamide-coated wooden tips coupled to direct infusion mass spectrometry, a high throughput alternative for the determination of methadone, cocaine and methamphetamine in oral fluid","volume":"162","author":"Lucena","year":"2021","journal-title":"Microchem. J."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"110330","DOI":"10.1016\/j.forsciint.2020.110330","article-title":"A new LC-MS\/MS confirmation method for the determination of 17 drugs of abuse in oral fluid and its application to real samples","volume":"312","author":"Bassotti","year":"2020","journal-title":"Forensic Sci. Int."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s00216-018-1428-5","article-title":"Simultaneous determination of 20 drugs of abuse in oral fluid using ultrasound-assisted dispersive liquid\u2013liquid microextraction","volume":"411","author":"Regenjo","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1093\/jat\/bky053","article-title":"Quantitative analysis of novel synthetic opioids, morphine and buprenorphine in oral fluid by LC\u2013MS-MS","volume":"42","author":"Truver","year":"2018","journal-title":"J. Anal. Toxicol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.talanta.2017.06.022","article-title":"A fast bioanalytical method based on microextraction by packed sorbent and UPLC\u2013MS\/MS for determining new psychoactive substances in oral fluid","volume":"174","author":"Ares","year":"2017","journal-title":"Talanta"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"360348","DOI":"10.1016\/j.chroma.2019.07.002","article-title":"Combination of pressurized liquid extraction with dispersive liquid liquid micro extraction for the determination of sixty drugs of abuse in hair","volume":"1605","author":"Vincenti","year":"2019","journal-title":"J. Chromatogr. A"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1093\/jat\/bkaa040","article-title":"Microextraction by packed sorbent as a novel strategy for sample clean-up in the determination of methadone and EDDP in hair","volume":"44","author":"Rosado","year":"2020","journal-title":"J. Anal. Toxicol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"184","DOI":"10.4103\/jfsm.jfsm_22_18","article-title":"Determination of methadone and eight new psychoactive substances in hair samples by gas chromatography\/mass spectrometry","volume":"4","author":"Anzillotti","year":"2018","journal-title":"J. Forensic. Sci. Med."},{"key":"ref_73","first-page":"400","article-title":"Distribution of methadone and metabolites in skeletal tissue","volume":"42","author":"Vandenbosch","year":"2018","journal-title":"J. Anal. Toxicol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1093\/jat\/bkab071","article-title":"Methadone, buprenorphine, oxycodone, fentanyl, and tramadol in multiple postmortem matrices","volume":"46","author":"Havig","year":"2022","journal-title":"J. Anal. Toxicol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.jpba.2017.10.003","article-title":"A liquid chromatography and tandem mass spectrometry method to determine 28 non-volatile drugs of abuse in exhaled breath","volume":"148","author":"Ullah","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"2342","DOI":"10.1039\/C7AY00110J","article-title":"Chiral separation of methadone in exhaled breath condensate using capillary electrophoresis","volume":"9","author":"Hamidi","year":"2017","journal-title":"Anal. Methods"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"116347","DOI":"10.1016\/j.trac.2021.116347","article-title":"Cathinones in urine samples: A review of recent advances for their determination by chromatographic and related techniques","volume":"143","author":"Borrull","year":"2021","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1053\/beem.2000.0060","article-title":"Techniques for analytical testing of unconventional samples","volume":"14","author":"Rivier","year":"2000","journal-title":"Best Pract. Res. Clin. Endocrinol. Metab."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/dta.1532","article-title":"Comparison of drug concentrations between whole blood and oral fluid","volume":"6","author":"Langel","year":"2014","journal-title":"Drug Test. Anal."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"295","DOI":"10.4155\/bio-2018-0208","article-title":"Green sample preparations for the bioanalysis of drugs of abuse in complex matrices","volume":"11","author":"Silveira","year":"2019","journal-title":"Bioanalysis"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"20150380","DOI":"10.1098\/rsta.2015.0380","article-title":"Quantitative mass spectrometry of unconventional human biological matrices","volume":"374","author":"Dutkiewicz","year":"2016","journal-title":"Philos. Trans. R. Soc. A"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1080\/10915810600683028","article-title":"Hair as a Biological Indicator of Drug Use, Drug Abuse or Chronic Exposure to Environmental Toxicants","volume":"25","author":"Boumba","year":"2006","journal-title":"Int. J. Toxicol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.microc.2017.02.032","article-title":"The effect of sampling procedures on the urate and lactate concentration in oral fluid","volume":"136","author":"Lomonaco","year":"2018","journal-title":"Microchem. J."},{"key":"ref_84","first-page":"12","article-title":"Vitreous humor analysis for the detection of xenobiotics in forensic toxicology: A review","volume":"34","author":"Cartiser","year":"2015","journal-title":"Forensic Toxicol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1007\/s00414-020-02494-8","article-title":"Detecting drugs in dry bone: A pilot study of skeletal remains with a post-mortem interval over 23 years","volume":"135","author":"Giordano","year":"2021","journal-title":"Int. J. Legal Med."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.rmed.2005.04.007","article-title":"Methodological improvements for measuring eicosanoids and cytokines in exhaled breath condensate","volume":"100","author":"Tufvesson","year":"2006","journal-title":"Resp. Med."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.foodchem.2017.10.005","article-title":"Simultaneous LC-MS\/MS determination of 40 legal and illegal psychoactive drugs in breast and bovine milk","volume":"245","author":"Mastroianni","year":"2018","journal-title":"Food Chem."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Shekari, A., Valipour, R., Setareh, M., and Soltaninejad, K. (2020). Research Paper: Ultrasound-assisted liquid-liquid extraction for analyzing methadone in urine samples by gas chromatography-mass spectrometry. Int. J. Med. Toxicol. Forensic Med., 10.","DOI":"10.32598\/ijmtfm.v10i3.29457"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"329","DOI":"10.34172\/apb.2020.040","article-title":"Validation and optimization of ultrasound-assisted dispersive liquid-liquid microextraction as a preparation method for detection of methadone in saliva with gas chromatography-mass spectrometry technique","volume":"10","author":"Shekari","year":"2020","journal-title":"Adv. Pharm. Bull."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1080\/00450618.2016.1153148","article-title":"Liquid chromatography tandem mass spectrometric method validation for the quantification of buprenorphine and norbuprenorphine in whole blood","volume":"49","author":"Chan","year":"2017","journal-title":"Aust. J. Forensic Sci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.jchromb.2017.01.024","article-title":"Ultrasound-assisted dispersive liquid-liquid microextraction for the determination of seven recreational drugs in human whole blood using gas chromatography-mass spectrometry","volume":"1046","author":"Lin","year":"2017","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4172\/2155-9872.1000368","article-title":"Rapid identification of buprenorphine in patient saliva","volume":"8","author":"Farquharson","year":"2017","journal-title":"J. Anal. Bioanal. Technol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.1002\/jssc.201700287","article-title":"Determination of seven drugs of abuse and their metabolites in surface and wastewater using solid-phase extraction coupled to liquid chromatography with high-resolution mass spectrometry","volume":"40","author":"Prosen","year":"2017","journal-title":"J. Sep. Sci."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1007\/s13738-018-1355-6","article-title":"Magnetic molecularly imprinted polymer nanoparticles for dispersive micro solid-phase extraction and determination of buprenorphine in human urine samples by HPLC-FL","volume":"15","author":"Habibi","year":"2018","journal-title":"J. Iran. Chem. Soc."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.snb.2016.08.007","article-title":"Enhanced electrochemical biosensing of buprenorphine opioid drugby highly stabilized magnetic nanocrystals","volume":"239","author":"Farmany","year":"2017","journal-title":"Sens. Actuators B"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.microc.2018.09.031","article-title":"Screening and quantitation of forty-six drugs of abuse and toxic compounds in human whole blood by capillary electrophoresis: Application to forensic cases","volume":"144","author":"Cui","year":"2019","journal-title":"Microchem. J."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/16\/5211\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:09:11Z","timestamp":1760141351000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/16\/5211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,16]]},"references-count":96,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["molecules27165211"],"URL":"https:\/\/doi.org\/10.3390\/molecules27165211","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,16]]}}}