{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:10:32Z","timestamp":1792789832703,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T00:00:00Z","timestamp":1662076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073611"],"award-info":[{"award-number":["82073611"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82002186"],"award-info":[{"award-number":["82002186"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["19KJB310002"],"award-info":[{"award-number":["19KJB310002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of the Higher Education Institutions of Jiangsu Province","award":["82073611"],"award-info":[{"award-number":["82073611"]}]},{"name":"Natural Science Foundation of the Higher Education Institutions of Jiangsu Province","award":["82002186"],"award-info":[{"award-number":["82002186"]}]},{"name":"Natural Science Foundation of the Higher Education Institutions of Jiangsu Province","award":["19KJB310002"],"award-info":[{"award-number":["19KJB310002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The CRISPR-Cas system is a bacterial and archaea adaptive immune system and is a newly recognized mechanism for controlling antibiotic resistance gene transfer. Acinetobacter baumannii (A. baumannii) is an important organism responsible for a variety of nosocomial infections. A. baumannii infections have become problematic worldwide because of the resistance of A. baumannii to multiple antibiotics. Thus, it is clinically significant to explore the relationship between the CRISPR-Cas system and drug resistance in A. baumannii. This study aimed to analyze the genomic characteristics of the A. baumannii strain AB3 containing the type I-Fb CRISPR-Cas system, which was isolated from a tertiary care hospital in China, and to investigate the relationship between the CRISPR-Cas system and antibiotic resistance in this strain. The whole-genome sequencing (WGS) of the AB43 strain was performed using Illumina and PacBio sequencing. The complete genome of AB43 consisted of a 3,854,806 bp chromosome and a 104,309 bp plasmid. The specific characteristics of the CRISPR-Cas system in AB43 are described as follows: (1) The strain AB43 carries a complete type I-Fb CRISPR-Cas system; (2) Homology analysis confirmed that the cas genes in AB43 share high sequence similarity with the same subtype cas genes; (3) A total of 28 of 105 A. baumannii AB43 CRISPR spacers matched genes in the bacteriophage genome database and the plasmid database, implying that the CRISPR-Cas system in AB43 provides immunity against invasive bacteriophage and plasmids; (4) None of the CRISPR spacers in A. baumannii AB43 were matched with antimicrobial resistance genes in the NCBI database. In addition, we analyzed the presence of antibiotic resistance genes and insertion sequences in the AB43 strain and found that the number of antibiotic resistance genes was not lower than in the \u201cno CRISPR-Cas system\u201d strain. This study supports the idea that the CRISPR-Cas system may inhibit drug-resistance gene expression via endogenous gene regulation, except to the published mechanism that the CRISPR-Cas system efficiently limits the acquisition of antibiotic resistance genes that make bacteria sensitive to antibiotics.<\/jats:p>","DOI":"10.3390\/molecules27175665","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T01:51:57Z","timestamp":1662515517000},"page":"5665","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Whole-Genome Analysis of Acinetobacter baumannii Strain AB43 Containing a Type I-Fb CRISPR-Cas System: Insights into the Relationship with Drug Resistance"],"prefix":"10.3390","volume":"27","author":[{"given":"Tingting","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Medicine, Yangzhou University, Yangzhou 225001, China"},{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou 225001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Yangzhou University, Yangzhou 225001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Yangzhou University, Yangzhou 225001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Yangzhou University, Yangzhou 225001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Yangzhou University, Yangzhou 225001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guimei","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Yangzhou University, Yangzhou 225001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Yangzhou University, Yangzhou 225001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyu","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Diagnosis, Affiliated Hospital of Yangzhou University, Yangzhou 225001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guocai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Yangzhou University, Yangzhou 225001, China"},{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou 225001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/s41426-018-0038-9","article-title":"Analysis of global prevalence of antibiotic resistance in Acinetobacter baumannii infections disclosed a faster increase in OECD countries","volume":"7","author":"Xie","year":"2018","journal-title":"Emerg. 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