{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T20:27:48Z","timestamp":1776198468258,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T00:00:00Z","timestamp":1662336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Slovak Research &amp; Development Agency","doi-asserted-by":"publisher","award":["APVV-19-0154"],"award-info":[{"award-number":["APVV-19-0154"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Slovak Research &amp; Development Agency","doi-asserted-by":"publisher","award":["2\/0053\/19"],"award-info":[{"award-number":["2\/0053\/19"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Slovak Research &amp; Development Agency","doi-asserted-by":"publisher","award":["2\/0091\/21"],"award-info":[{"award-number":["2\/0091\/21"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"name":"VEGA Grant Agency of the Slovak Republic","award":["APVV-19-0154"],"award-info":[{"award-number":["APVV-19-0154"]}]},{"name":"VEGA Grant Agency of the Slovak Republic","award":["2\/0053\/19"],"award-info":[{"award-number":["2\/0053\/19"]}]},{"name":"VEGA Grant Agency of the Slovak Republic","award":["2\/0091\/21"],"award-info":[{"award-number":["2\/0091\/21"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Aqueous root extract from Acanthopanax senticosus (ASRE) has a wide range of medicinal effects. The present work was aimed at studying the influence of sulfide, cysteine and glutathione on the antioxidant properties of ASRE and some of its selected phytochemical components. Reduction of the 2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazol-1-yloxy-3-oxide (\u25cfcPTIO) stable radical and plasmid DNA (pDNA) cleavage in vitro assays were used to evaluate antioxidant and DNA-damaging properties of ASRE and its individual components. We found that the interaction of ASRE and its two components, caffeic acid and chlorogenic acid (but not protocatechuic acid and eleutheroside B or E), with H2S\/HS\u2212, cysteine or glutathione significantly increased the reduction of the \u25cfcPTIO radical. In contrast, the potency of ASRE and its selected components was not affected by Na2S4, oxidized glutathione, cystine or methionine, indicating that the thiol group is a prerequisite for the promotion of the antioxidant effects. ASRE interacting with H2S\/HS\u2212 or cysteine displayed a bell-shaped effect in the pDNA cleavage assay. However, ASRE and its components inhibited pDNA cleavage induced by polysulfides. In conclusion, we suggest that cysteine, glutathione and H2S\/HS\u2212 increase antioxidant properties of ASRE and that changes of their concentrations and the thiol\/disulfide ratio can influence the resulting biological effects of ASRE.<\/jats:p>","DOI":"10.3390\/molecules27175735","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T01:51:57Z","timestamp":1662515517000},"page":"5735","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Extract of Acanthopanax senticosus and Its Components Interacting with Sulfide, Cysteine and Glutathione Increase Their Antioxidant Potencies and Inhibit Polysulfide-Induced Cleavage of Plasmid DNA"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6331-1004","authenticated-orcid":false,"given":"Anton","family":"Misak","sequence":"first","affiliation":[{"name":"Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2846-7924","authenticated-orcid":false,"given":"Marian","family":"Grman","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1943-0858","authenticated-orcid":false,"given":"Lenka","family":"Tomasova","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ondrej","family":"Makara","sequence":"additional","affiliation":[{"name":"Forest Arboretum Liptovsky Hradok, Hradna 534, 033 01 Liptovsky Hradok, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9120-5084","authenticated-orcid":false,"given":"Miroslav","family":"Chovanec","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8329-3563","authenticated-orcid":false,"given":"Karol","family":"Ondrias","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Panossian, A., and Brendler, T. 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