{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:30:04Z","timestamp":1792042204089,"version":"4.4.0"},"reference-count":105,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,11]],"date-time":"2022-09-11T00:00:00Z","timestamp":1662854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Turkish Academy of Sciences (T\u00dcBA)","award":["RSP-2022\/59"],"award-info":[{"award-number":["RSP-2022\/59"]}]},{"name":"King Saud University\u2019s Researchers Supporting Project","award":["RSP-2022\/59"],"award-info":[{"award-number":["RSP-2022\/59"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Magnofluorine, a secondary metabolite commonly found in various plants, has pharmacological potential; however, its antioxidant and enzyme inhibition effects have not been investigated. We investigated the antioxidant potential of Magnofluorine using bioanalytical assays with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS\u2022+), N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD\u2022+), and 1,1-diphenyl-2-picrylhydrazyl (DPPH\u2022) scavenging abilities and K3[Fe(CN)6] and Cu2+ reduction abilities. Further, we compared the effects of Magnofluorine and butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), \u03b1-Tocopherol, and Trolox as positive antioxidant controls. According to the analysis results, Magnofluorine removed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals with an IC50 value of 10.58 \u03bcg\/mL. The IC50 values of BHA, BHT, Trolox, and \u03b1-Tocopherol were 10.10 \u03bcg\/mL, 25.95 \u03bcg\/mL, 7.059 \u03bcg\/mL, and 11.31 \u03bcg\/mL, respectively. Our results indicated that the DPPH\u0387 scavenging effect of Magnofluorine was similar to that of BHA, close to that of Trolox, and better than that of BHT and \u03b1-tocopherol. The inhibition effect of Magnofluorine was examined against enzymes, such as acetylcholinesterase (AChE), \u03b1-glycosidase, butyrylcholinesterase (BChE), and human carbonic anhydrase II (hCA II), which are linked to global disorders, such as diabetes, Alzheimer\u2019s disease (AD), and glaucoma. Magnofluorine inhibited these metabolic enzymes with Ki values of 10.251.94, 5.991.79, 25.411.10, and 30.563.36 nM, respectively. Thus, Magnofluorine, which has been proven to be an antioxidant, antidiabetic, and anticholinergic in our study, can treat glaucoma. In addition, molecular docking was performed to understand the interactions between Magnofluorine and target enzymes BChE (D: 6T9P), hCA II (A:3HS4), AChE (B:4EY7), and \u03b1-glycosidase (C:5NN8). The results suggest that Magnofluorine may be an important compound in the transition from natural sources to industrial applications, especially new drugs.<\/jats:p>","DOI":"10.3390\/molecules27185902","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T01:44:03Z","timestamp":1663033443000},"page":"5902","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Antioxidant, Antidiabetic, Anticholinergic, and Antiglaucoma Effects of Magnofluorine"],"prefix":"10.3390","volume":"27","author":[{"given":"Lokman","family":"Durmaz","sequence":"first","affiliation":[{"name":"Department of Medical Services and Technology, Cayirli Vocational School, Erzincan Binali Yildirim University, Erzincan 24500, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatice","family":"Kiziltas","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Services, Vocational School of Health Services, Van Yuzuncu Yil University, Van 65080, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyla","family":"Guven","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, Ataturk University, Erzurum 25240, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6912-3998","authenticated-orcid":false,"given":"Hasan","family":"Karagecili","sequence":"additional","affiliation":[{"name":"Department of Nursing, Faculty of Health Science, Siirt University, Siirt 56100, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saleh","family":"Alwasel","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science, King Saud University, Riyadh 11362, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5993-1668","authenticated-orcid":false,"given":"\u0130lhami","family":"Gulcin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Ataturk University, Erzurum 25240, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00204-020-02689-3","article-title":"Antioxidants and antioxidant methods-An updated overview","volume":"94","author":"Gulcin","year":"2020","journal-title":"Arch. 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