{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:31:29Z","timestamp":1791930689116,"version":"4.3.4"},"reference-count":78,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T00:00:00Z","timestamp":1663372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004748","name":"Mashhad University of Medical Sciences","doi-asserted-by":"publisher","award":["980077"],"award-info":[{"award-number":["980077"]}],"id":[{"id":"10.13039\/501100004748","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Domp\u00e9 Farmaceutici S.p.A fellowship for PhD program in \u201cNutraceuticals, functional foods and human health\u201d","award":["980077"],"award-info":[{"award-number":["980077"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Molecules"],"abstract":"<jats:p>Background: Type-one diabetes (T1D), a chronic autoimmune disease with marked inflammatory responses, is associated with infertility complications and implications. Based on the anti-diabetic, antioxidant, and anti-hyperlipidemic potential of Portulaca oleracea (PO), this study aimed to evaluate the protective effect of this plant extract on streptozotocin-induced type-I-diabetes-associated reproductive system dysfunction and inflammation. Methods: Male rats were randomly divided into four experimental groups: control, diabetic, and treatment\/s (PO extract at 100 or 300 mg\/kg\/daily). Then food and water consumption, body, testis and epididymis weights, histopathological evaluation, seminiferous tubules diameter, sperm count and motility, glucose levels, sex hormones, and inflammatory and oxidative stress markers were evaluated. Results: Our results showed that streptozotocin-induced diabetes significantly increased food and water consumption; increased glucose, MDA, TGF-\u03b21, and TNF-\u03b1 levels; and decreased the seminiferous tubules diameter, sperm count and motility, levels of LH, testosterone, total thiol, VEGF, and SOD activity. Interestingly, PO extract (phytochemically characterized by using liquid chromatography\u2013mass spectrometry to detect bioactive molecules) significantly ameliorated these parameters and histopathological indexes\u2019 damage in rats. Conclusion. Even if more preclinical assessments are needed to better characterize the mechanism\/s of action, the results of this study will pave the way for the rational use of PO on diabetic-associated clinical complications and implications.<\/jats:p>","DOI":"10.3390\/molecules27186075","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T02:06:50Z","timestamp":1663553210000},"page":"6075","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Protective Effect of Portulaca oleracea on Streptozotocin-Induced Type I Diabetes-Associated Reproductive System Dysfunction and Inflammation"],"prefix":"10.3390","volume":"27","author":[{"given":"Hassan","family":"Rakhshandeh","sequence":"first","affiliation":[{"name":"Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamed","family":"Rajabi Khasevan","sequence":"additional","affiliation":[{"name":"Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anella","family":"Saviano","sequence":"additional","affiliation":[{"name":"ImmunoPharmaLab, Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5724-7651","authenticated-orcid":false,"given":"Mohammad Reza","family":"Mahdinezhad","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vafa","family":"Baradaran Rahimi","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Diseases, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajjad","family":"Ehtiati","sequence":"additional","affiliation":[{"name":"Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"},{"name":"Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Etemad","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"},{"name":"Department of Drug Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Ebrahimzadeh-bideskan","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Pardis Campus, Azadi Square, Mashhad 9177948564, Iran"},{"name":"Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2280-4299","authenticated-orcid":false,"given":"Francesco","family":"Maione","sequence":"additional","affiliation":[{"name":"ImmunoPharmaLab, Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9268-6270","authenticated-orcid":false,"given":"Vahid Reza","family":"Askari","sequence":"additional","affiliation":[{"name":"ImmunoPharmaLab, Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy"},{"name":"Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"},{"name":"Department of Pharmaceutical Sciences in Persian Medicine, School of Persian and Complementary Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"},{"name":"International UNESCO Center for Health-Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,17]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Introduction to diabetes mellitus","volume":"771","author":"Kaul","year":"2012","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"107843","DOI":"10.1016\/j.diabres.2019.107843","article-title":"Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition","volume":"157","author":"Saeedi","year":"2019","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1038\/nrendo.2009.21","article-title":"The role of inflammation in insulitis and beta-cell loss in type 1 diabetes","volume":"5","author":"Eizirik","year":"2009","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/nrendo.2017.151","article-title":"Global aetiology and epidemiology of type 2 diabetes mellitus and its complications","volume":"14","author":"Zheng","year":"2018","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"948","DOI":"10.4103\/1008-682X.150844","article-title":"The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis","volume":"17","author":"Ding","year":"2015","journal-title":"Asian J. Androl."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1111\/jsm.12601","article-title":"Sexual dysfunction at the onset of type 2 diabetes: The interplay of depression, hormonal and cardiovascular factors","volume":"11","author":"Corona","year":"2014","journal-title":"J. Sex. Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Baradaran Rahimi, V., Rakhshandeh, H., Raucci, F., Buono, B., Shirazinia, R., Samzadeh Kermani, A., Maione, F., Mascolo, N., and Askari, V.R. (2019). Anti-Inflammatory and Anti-Oxidant Activity of Portulaca oleracea Extract on LPS-Induced Rat Lung Injury. Molecules, 24.","DOI":"10.3390\/molecules24010139"},{"key":"ref_8","first-page":"165","article-title":"Cytotoxicity and apoptogenic properties of the standardized extract of Portulaca oleracea on glioblastoma multiforme cancer cell line (U-87): A mechanistic study","volume":"18","author":"Mousavi","year":"2019","journal-title":"EXCLI J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3831\/KPI.2019.22.001","article-title":"A Pharmacological Review on Portulaca oleracea L.: Focusing on Anti-Inflammatory, Anti-Oxidant, Immuno-Modulatory and Antitumor Activities","volume":"22","author":"Rahimi","year":"2019","journal-title":"J. Pharmacopunct."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.msard.2018.07.015","article-title":"Protective effects of hydro-ethanolic extract of Terminalia chebula on primary microglia cells and their polarization (M1\/M2 balance)","volume":"25","author":"Rahimi","year":"2018","journal-title":"Mult. Scler. Relat. Disord."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.sajb.2021.07.033","article-title":"Promising anti-melanogenic impacts of Portulaca oleracea on B16F1 murine melanoma cell line: An in-vitro vision","volume":"142","author":"Askari","year":"2021","journal-title":"S. Afr. J. Bot."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8437753","DOI":"10.1155\/2021\/8437753","article-title":"Evaluation of the Therapeutic Effects of the Hydroethanolic Extract of Portulaca oleracea on Surgical-Induced Peritoneal Adhesion","volume":"2021","author":"Jaafari","year":"2021","journal-title":"Mediat. Inflamm."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"925631","DOI":"10.1155\/2015\/925631","article-title":"Portulaca oleracea L.: A review of phytochemistry and pharmacological effects","volume":"2015","author":"Zhou","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bai, Y., Zang, X., Ma, J., and Xu, G. (2016). Anti-Diabetic Effect of Portulaca oleracea L. Polysaccharideandits Mechanism in Diabetic Rats. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17081201"},{"key":"ref_15","first-page":"867","article-title":"Hydro-ethanolic Extract of Portulaca oleracea Affects Beta-adrenoceptors of Guinea Pig Tracheal Smooth Muscle","volume":"15","author":"Boskabady","year":"2016","journal-title":"Iran. J. Pharm. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.phytochem.2012.05.014","article-title":"Homoisoflavonoids from the medicinal plant Portulaca oleracea","volume":"80","author":"Yan","year":"2012","journal-title":"Phytochemistry"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S0021-9673(03)00786-6","article-title":"Determination of noradrenaline and dopamine in Chinese herbal extracts from Portulaca oleracea L. by high-performance liquid chromatography","volume":"1003","author":"Chen","year":"2003","journal-title":"J. Chromatogr. A"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1016\/j.phytochem.2005.08.011","article-title":"Alkaloids from Portulaca oleracea L.","volume":"66","author":"Xiang","year":"2005","journal-title":"Phytochemistry"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1080\/10286020.2013.866948","article-title":"Two new alkaloids from Portulaca oleracea and their cytotoxic activities","volume":"16","author":"Tian","year":"2014","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.talanta.2013.11.067","article-title":"Rapid determination of eight bioactive alkaloids in Portulaca oleracea L. by the optimal microwave extraction combined with positive-negative conversion multiple reaction monitor (+\/\u2212MRM) technology","volume":"120","author":"Liang","year":"2014","journal-title":"Talanta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1016\/0031-9422(96)00202-6","article-title":"Portuloside A, a monoterpene glucoside, from Portulaca oleracea","volume":"42","author":"Sakai","year":"1996","journal-title":"Phytochemistry"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1080\/10286020802320590","article-title":"Portulene, a new diterpene from Portulaca oleracea L.","volume":"10","author":"Elkhayat","year":"2008","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.5012\/bkcs.2003.24.10.1475","article-title":"A new monoterpene glucoside from Portulaca oleracea","volume":"24","author":"Seo","year":"2003","journal-title":"Bull. Korean Chem. Soc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1002\/hlca.200890221","article-title":"Two Novel Triterpenoids from Portulaca oleracea L.","volume":"91","author":"Xin","year":"2008","journal-title":"Helv. Chim. Acta"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.jpba.2006.01.013","article-title":"Determination of flavonoids in Portulaca oleracea L. by capillary electrophoresis with electrochemical detection","volume":"41","author":"Xu","year":"2006","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1007\/s12161-009-9091-2","article-title":"Analysis of Flavonoids in Portulaca oleracea L. by UV\u2013Vis Spectrophotometry with Comparative Study on Different Extraction Technologies","volume":"3","author":"Zhu","year":"2010","journal-title":"Food Anal. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3490","DOI":"10.1021\/jf0102113","article-title":"Stage of harvest and polyunsaturated essential fatty acid concentrations in purslane (Portulaca oleraceae) leaves","volume":"49","author":"Palaniswamy","year":"2001","journal-title":"J. Agric. Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1002\/ptr.2742","article-title":"Phenolic alkaloids as a new class of antioxidants in Portulaca oleracea","volume":"23","author":"Yang","year":"2009","journal-title":"Phytother. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"951019","DOI":"10.1155\/2014\/951019","article-title":"Purslane weed (Portulaca oleracea): A prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes","volume":"2014","author":"Uddin","year":"2014","journal-title":"Sci. World J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1080\/14786419.2011.648193","article-title":"LC determination and pharmacokinetic study of the main phenolic components of Portulaca oleracea L. extract in rat plasma after oral administration","volume":"26","author":"Cheng","year":"2012","journal-title":"Nat. Prod. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.ejphar.2011.03.030","article-title":"A study comparing the effects of rosiglitazone and\/or insulin treatments on streptozotocin induced diabetic (type I diabetes) rat aorta and cavernous tissues","volume":"660","author":"Kabasakal","year":"2011","journal-title":"Eur. J. Pharmacol."},{"key":"ref_32","first-page":"5","article-title":"Streptozotocin-Induced Diabetic Models in Mice and Rats","volume":"70","author":"Furman","year":"2015","journal-title":"Curr. Protoc. Pharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/BF02913315","article-title":"Induction of diabetes by Streptozotocin in rats","volume":"22","author":"Akbarzadeh","year":"2007","journal-title":"Indian J. Clin. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1016\/j.jep.2011.08.059","article-title":"Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats","volume":"137","author":"Vasconcelos","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"741824","DOI":"10.1155\/2012\/741824","article-title":"Portulaca oleracea Ameliorates Diabetic Vascular Inflammation and Endothelial Dysfunction in db\/db Mice","volume":"2012","author":"Lee","year":"2012","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ramadan, B.K., Schaalan, M.F., and Tolba, A.M. (2017). Hypoglycemic and pancreatic protective effects of Portulaca oleracea extract in alloxan induced diabetic rats. BMC Complement. Altern. Med., 17.","DOI":"10.1186\/s12906-016-1530-1"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"411","DOI":"10.4297\/najms.2011.3411","article-title":"The antiatherogenic, renal protective and immunomodulatory effects of purslane, pumpkin and flax seeds on hypercholesterolemic rats","volume":"3","author":"Barakat","year":"2011","journal-title":"N. Am. J. Med. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/j.1439-0272.2009.00937.x","article-title":"Diabetic rat testes: Morphological and functional alterations","volume":"41","author":"Ricci","year":"2009","journal-title":"Andrologia"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"26","DOI":"10.5603\/FHC.a2015.0006","article-title":"The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: Biochemical and immunohistochemical study","volume":"53","author":"Karabulut","year":"2015","journal-title":"Folia Histochem. Cytobiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"017","DOI":"10.1055\/s-0039-1683405","article-title":"Effect of Diabetes on the Male Reproductive System\u2014A Histomorphological Study","volume":"36","author":"Kotian","year":"2019","journal-title":"J. Morphol. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1002\/ptr.3100","article-title":"Beneficial effects of quercetin on sperm parameters in streptozotocin-induced diabetic male rats","volume":"24","author":"Khaki","year":"2010","journal-title":"Phytother. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"153573","DOI":"10.1155\/2015\/153573","article-title":"Ameliorative Effect of Zinc Oxide Nanoparticles on Antioxidants and Sperm Characteristics in Streptozotocin-Induced Diabetic Rat Testes","volume":"2015","author":"Afifi","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"21","DOI":"10.5455\/ajpbp.20190124101830","article-title":"Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats","volume":"9","author":"Obinna","year":"2019","journal-title":"Am. J. Physiol. Biochem. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.bbrc.2014.05.067","article-title":"Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model","volume":"450","author":"Xu","year":"2014","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6","DOI":"10.14715\/cmb\/2018.64.2.2","article-title":"Effect of Stevia rebaudiana Bertoni extract on sexual dysfunction in Streptozotocin-induced diabetic male rats","volume":"64","author":"Ghaheri","year":"2018","journal-title":"Cell. Mol. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1080\/17512433.2018.1445969","article-title":"Pharmacological management of late-onset hypogonadism","volume":"11","author":"Rastrelli","year":"2018","journal-title":"Expert Rev. Clin. Pharmacol."},{"key":"ref_47","unstructured":"Aladamat, N., and Tadi, P. (2021). Histology, Leydig Cells. StatPearls, StatPearls Publishing LLC. StatPearls Publishing Copyright \u00a9 2022."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Aci\u00e9n, P., and Aci\u00e9n, M. (2020). Disorders of Sex Development: Classification, Review, and Impact on Fertility. J. Clin. Med., 9.","DOI":"10.3390\/jcm9113555"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1002\/j.1939-4640.2004.tb02845.x","article-title":"Insulin-dependent diabetes affects testicular function by FSH- and LH-linked mechanisms","volume":"25","author":"Ballester","year":"2004","journal-title":"J. Androl."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Farag, O.M., Abd-Elsalam, R.M., El Badawy, S.A., Ogaly, H.A., Alsherbiny, M.A., and Ahmed, K.A. (2021). Portulaca oleracea seeds\u2019 extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats. BMC Complement. Med. Ther., 21.","DOI":"10.1186\/s12906-021-03286-2"},{"key":"ref_51","first-page":"2550","article-title":"Effects of ethanolic purslane shoot and seed extracts on doxorubicin-induced testicular toxicity in albino rats","volume":"10","author":"Hozayen","year":"2013","journal-title":"Life Sci. J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1007\/s43440-020-00096-0","article-title":"Fenofibrate ameliorates testicular damage in rats with streptozotocin-induced type 1 diabetes: Role of HO-1 and p38 MAPK","volume":"72","author":"Abozaid","year":"2020","journal-title":"Pharmacol. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.reprotox.2007.02.001","article-title":"Early oxidative stress in testis and epididymal sperm in streptozotocin-induced diabetic mice: Its progression and genotoxic consequences","volume":"23","author":"Shrilatha","year":"2007","journal-title":"Reprod. Toxicol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1111\/j.1365-2605.2007.00748.x","article-title":"Occurrence of oxidative impairments, response of antioxidant defences and associated biochemical perturbations in male reproductive milieu in the Streptozotocin-diabetic rat","volume":"30","author":"Shrilatha","year":"2007","journal-title":"Int. J. Androl."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1177\/2156587217692491","article-title":"Attenuation of Oxidative Stress and Inflammation by Portulaca oleracea in Streptozotocin-Induced Diabetic Rats","volume":"22","author":"Samarghandian","year":"2017","journal-title":"J. Evid. Based Complement. Altern. Med."},{"key":"ref_56","first-page":"2138","article-title":"Protective effects of ethanol extract from Portulaca oleracea L on dextran sulphate sodium-induced mice ulcerative colitis involving anti-inflammatory and antioxidant","volume":"8","author":"Yang","year":"2016","journal-title":"Am. J. Transl. Res."},{"key":"ref_57","first-page":"205","article-title":"Effects of Portulaca oleracea ethanolic extract on reproductive system of aging female mice","volume":"14","author":"Ahangarpour","year":"2016","journal-title":"Int. J. Reprod. Biomed."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"177","DOI":"10.4103\/ijp.IJP_737_16","article-title":"The effect of Portulaca oleracea and \u03b1-linolenic acid on oxidant\/antioxidant biomarkers of human peripheral blood mononuclear cells","volume":"50","author":"Askari","year":"2018","journal-title":"Indian J. Pharmacol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1111\/j.1439-0272.2011.01245.x","article-title":"\u00caxpression of inducible nitric oxide synthase (iNOS) in the azoospermic human testis","volume":"44","author":"Filiz","year":"2012","journal-title":"Andrologia"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.4103\/1673-5374.235024","article-title":"Vascular endothelial growth factor: An essential neurotrophic factor for motoneurons?","volume":"13","author":"Calvo","year":"2018","journal-title":"Neural Regen. Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"50","DOI":"10.15420\/ecr.2018.33.1","article-title":"The Role of Inflammation in Diabetes: Current Concepts and Future Perspectives","volume":"14","author":"Tsalamandris","year":"2019","journal-title":"Eur. Cardiol. Rev."},{"key":"ref_62","first-page":"123","article-title":"Impact of Inflammation on Male Reproductive Tract","volume":"16","author":"Azenabor","year":"2015","journal-title":"J. Reprod. Infertil."},{"key":"ref_63","first-page":"89","article-title":"Impact of inflammation on male fertility","volume":"3","author":"Sarkar","year":"2011","journal-title":"Front. Biosci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1002\/j.1939-4640.2003.tb02693.x","article-title":"Male genital tract inflammation: The role of selected interleukins in regulation of pro-oxidant and antioxidant enzymatic substances in seminal plasma","volume":"24","author":"Sanocka","year":"2003","journal-title":"J. Androl."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"7517","DOI":"10.1007\/s11033-020-05814-8","article-title":"Modulation of reproductive dysfunctions associated with streptozocin-induced diabetes by Artemisia judaica extract in rats fed a high-fat diet","volume":"47","author":"Albasher","year":"2020","journal-title":"Mol. Biol. Rep."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.biopha.2016.12.102","article-title":"The antioxidant role of agomelatine and gallic acid on oxidative stress in STZ induced type I diabetic rat testes","volume":"87","author":"Yigitturk","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.ygcen.2018.04.007","article-title":"Vascular endothelial growth factor (VEGF-A) and fibroblast growth factor (FGF-2) as potential regulators of seasonal reproductive processes in male European bison (Bison bonasus, Linnaeus 1758)","volume":"263","author":"Mytych","year":"2018","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1095\/biolreprod.102.009795","article-title":"Regulation of vascular endothelial growth factor production by Leydig cells in vitro: The role of protein kinase A and mitogen-activated protein kinase cascade","volume":"68","author":"Anand","year":"2003","journal-title":"Biol. Reprod."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"108106","DOI":"10.1155\/2014\/108106","article-title":"Potential novel biomarkers for diabetic testicular damage in streptozotocin-induced diabetic rats: Nerve growth factor Beta and vascular endothelial growth factor","volume":"2014","author":"Sisman","year":"2014","journal-title":"Dis. Markers"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"5321","DOI":"10.18632\/oncotarget.23915","article-title":"Hyperglycemia induced testicular damage in type 2 diabetes mellitus rats exhibiting microcirculation impairments associated with vascular endothelial growth factor decreased via PI3K\/Akt pathway","volume":"9","author":"Long","year":"2018","journal-title":"Oncotarget"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.fertnstert.2005.01.144","article-title":"Protective effect of vascular endothelial growth factor on histologic changes in testicular ischemia-reperfusion injury","volume":"84","author":"Cayan","year":"2005","journal-title":"Fertil. Steril."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1177\/1753425921994190","article-title":"Anti-nociceptive effect of Portulaca oleracea L. ethanol extracts attenuated zymosan-induced mouse joint inflammation via inhibition of Nrf2 expression","volume":"27","author":"He","year":"2021","journal-title":"Innate Immun."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"116595","DOI":"10.1016\/j.lfs.2019.116595","article-title":"Ellagic acid dose and time-dependently abrogates d-galactose-induced animal model of aging: Investigating the role of PPAR-gamma","volume":"232","author":"Askari","year":"2019","journal-title":"Life Sci."},{"key":"ref_74","first-page":"e1918","article-title":"Linearization of the bradford protein assay","volume":"38","author":"Ernst","year":"2010","journal-title":"JoVE J. Vis. Exp."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"111579","DOI":"10.1016\/j.biopha.2021.111579","article-title":"Supplementation with ribonucleotide-based ingredient (Ribodiet\u00ae) lessens oxidative stress, brain inflammation, and amyloid pathology in a murine model of Alzheimer","volume":"139","author":"Saviano","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1007\/s43440-020-00083-5","article-title":"Anti-oxidant and anti-inflammatory effects of auraptene on phytohemagglutinin (PHA)-induced inflammation in human lymphocytes","volume":"73","author":"Askari","year":"2021","journal-title":"Pharmacol. Rep."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1007\/s10787-021-00819-6","article-title":"Standardised pomegranate peel extract lavage prevents postoperative peritoneal adhesion by regulating TGF-\u03b2 and VEGF levels","volume":"29","author":"Ghadiri","year":"2021","journal-title":"Inflammopharmacology"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"4","DOI":"10.4103\/abr.abr_124_18","article-title":"Effects of Crocin on Sperm Parameters and Seminiferous Tubules in Diabetic Rats","volume":"8","author":"Roshankhah","year":"2019","journal-title":"Adv. Biomed. Res."}],"updated-by":[{"DOI":"10.3390\/molecules30030449","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T00:00:00Z","timestamp":1663372800000}}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/18\/6075\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T13:46:02Z","timestamp":1754228762000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/18\/6075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,17]]},"references-count":78,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["molecules27186075"],"URL":"https:\/\/doi.org\/10.3390\/molecules27186075","relation":{"correction":[{"id-type":"doi","id":"10.3390\/molecules30030449","asserted-by":"object"}]},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,17]]}}}