{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:39:20Z","timestamp":1792805960303,"version":"4.5.0"},"reference-count":40,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T00:00:00Z","timestamp":1663372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the General Scientific Research Project of Zhejiang Provincial Department of Education","award":["Y202146772"],"award-info":[{"award-number":["Y202146772"]}],"id":[{"id":"https:\/\/ror.org\/059md9404","id-type":"ROR","asserted-by":"crossref"}]},{"name":"the General Scientific Research Project of Zhejiang Provincial Department of Education","award":["XZ-SZ202134"],"award-info":[{"award-number":["XZ-SZ202134"]}],"id":[{"id":"https:\/\/ror.org\/059md9404","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Jiangsu Provincial Policy Guidance Plan (North Jiangsu Science and Technology Project)","award":["Y202146772"],"award-info":[{"award-number":["Y202146772"]}]},{"name":"Jiangsu Provincial Policy Guidance Plan (North Jiangsu Science and Technology Project)","award":["XZ-SZ202134"],"award-info":[{"award-number":["XZ-SZ202134"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Pear fruits have been reported to contain abundant bioactive compounds and exhibit antidiabetic activity. In this study, Pingguoli pear (Pyrus pyrifolia cv.\u2018Pingguoli\u2019) fermentation broth was sequentially extracted by five solvents with increasing polarity (petroleum ether, chloroform, ethyl acetate, n-butanol, and water) to evaluate its antioxidant and hypothermic activities, and then the main compounds of the fraction with the highest activity were assessed, which might be responsible for such activities. The results showed that the ethyl acetate fraction (EAF) exhibited the highest antioxidant activity according to DPPH (IC50 = 0.238 mg\/mL), ABTS (IC50 = 0.293 mg\/mL), and FRAP (IC50 = 0.193 mg\/mL) assays. The in vitro hypoglycemic activity assay showed that EAF exhibited the strongest inhibitory effect, with IC50 values of 0.34 and 0.95 mg\/mL for \u03b1-amylase and \u03b1-glucosidase, respectively. The glucose consumption in HepG2 cells treated with EAF was significantly increased to 252%, compare with control group. Liquid chromatography\u2013mass spectrometry analysis implied that the main compounds, 3\u2032-C-glucosylisoliquiritigenin, robustside D, caffeic acid, and chlorogenic acid may be potential candidates for the antioxidant and hypoglycemic activities of the EAF. This study suggested that EAF of Pingguoli pear fermentation broth could be utilized for development of potential functional food and antidiabetic agents.<\/jats:p>","DOI":"10.3390\/molecules27186077","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T02:06:50Z","timestamp":1663553210000},"page":"6077","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Antioxidant and Hypoglycemic Activity of Sequentially Extracted Fractions from Pingguoli Pear Fermentation Broth and Identification of Bioactive Compounds"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5969-6063","authenticated-orcid":false,"given":"Jing","family":"Dai","sequence":"first","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"},{"name":"Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Products, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Si","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Gu","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuqian","family":"Xiao","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"},{"name":"Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Products, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Ge","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"},{"name":"Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Products, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3505-1225","authenticated-orcid":false,"given":"Ruyi","family":"Sha","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"},{"name":"Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Products, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Sivamaruthi, B.S., Kesika, P., Prasanth, M.I., and Chaiyasut, C. 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