{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T21:41:41Z","timestamp":1789594901349,"version":"build-2803163510"},"reference-count":30,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hunan Province Jiapinjiawei Biotechnology Co., Ltd."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study was conducted to analyze volatile odor compounds and key odor-active compounds in the fish soup using fish scarp and bone. Five extraction methods, including solid-phase microextraction (SPME), dynamic headspace sampling (DHS), solvent-assisted flavor evaporation (SAFE), stir bar sorptive extraction (SBSE), liquid-liquid extraction (LLE), were compared and SPME was finally selected as the best extraction method for further study. The volatile odor compounds were analyzed by gas chromatography-olfactometry-mass spectrometry (GC-O-MS) and comprehensive two-dimensional gas chromatography-olfactometry-mass spectrometry (GC \u00d7 GC-O-MS) techniques, and the key odor-active compounds were identified via aroma extract dilution analysis (AEDA) and relative odor activity value (r-OAV) calculation. A total of 38 volatile compounds were identified by GC-O-MS, among which 10 were declared as odor-active compounds. Whereas 39 volatile compounds were identified by GC \u00d7 GC-O-MS, among which 12 were declared as odor-active compounds. The study results revealed that 1-octen-3-one, 2-pentylfuran, (E)-2-octenal, 1-octen-3-one, hexanal, 1-octen-3-ol, 6-methylhept-5-en-2-one, (E,Z)-2,6-nondienal and 2-ethyl-3,5-dimethylpyrazine were the key odor-active compounds in the fish soup.<\/jats:p>","DOI":"10.3390\/molecules27196177","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:08:09Z","timestamp":1663718889000},"page":"6177","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Comparison of Different Volatile Extraction Methods for the Identification of Fishy Off-Odor in Fish By-Products"],"prefix":"10.3390","volume":"27","author":[{"given":"Yuanyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Sensory Science, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Tang","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Sensory Science, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Sensory Science, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8513-3299","authenticated-orcid":false,"given":"Huanlu","family":"Song","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Sensory Science, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Raza","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Sensory Science, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Pan","sequence":"additional","affiliation":[{"name":"Hunan Province Jiapinjiawei Biotechnology Co., Ltd, Changde 415401, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Gong","sequence":"additional","affiliation":[{"name":"Hunan Province Jiapinjiawei Biotechnology Co., Ltd, Changde 415401, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Jiang","sequence":"additional","affiliation":[{"name":"Wuzhou Testing Co., Ltd, Jining 273200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Coppola, D., Lauritano, C., Esposito, F., Riccio, G., and Pascale, D. 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