{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T14:26:26Z","timestamp":1783002386410,"version":"3.54.5"},"reference-count":25,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Nature Science Foundation of China","award":["82173997"],"award-info":[{"award-number":["82173997"]}]},{"name":"National Nature Science Foundation of China","award":["81703724"],"award-info":[{"award-number":["81703724"]}]},{"name":"National Nature Science Foundation of China","award":["2018jkcy04"],"award-info":[{"award-number":["2018jkcy04"]}]},{"name":"Health Industry Special Project of Heilongjiang University of Chinese Medicine","award":["82173997"],"award-info":[{"award-number":["82173997"]}]},{"name":"Health Industry Special Project of Heilongjiang University of Chinese Medicine","award":["81703724"],"award-info":[{"award-number":["81703724"]}]},{"name":"Health Industry Special Project of Heilongjiang University of Chinese Medicine","award":["2018jkcy04"],"award-info":[{"award-number":["2018jkcy04"]}]},{"name":"Heilong-jiang Touyan Innovation Team Program","award":["82173997"],"award-info":[{"award-number":["82173997"]}]},{"name":"Heilong-jiang Touyan Innovation Team Program","award":["81703724"],"award-info":[{"award-number":["81703724"]}]},{"name":"Heilong-jiang Touyan Innovation Team Program","award":["2018jkcy04"],"award-info":[{"award-number":["2018jkcy04"]}]},{"name":"Postdoctoral Research Foundation of Heilongjiang Province","award":["82173997"],"award-info":[{"award-number":["82173997"]}]},{"name":"Postdoctoral Research Foundation of Heilongjiang Province","award":["81703724"],"award-info":[{"award-number":["81703724"]}]},{"name":"Postdoctoral Research Foundation of Heilongjiang Province","award":["2018jkcy04"],"award-info":[{"award-number":["2018jkcy04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The decoction turns into a complex multiphase system following exposure to high temperature and a complex chemical environment. However, the differences in the concentration of key active ingredients in different phase states and the release of drugs in sedimentary phase have yet to be elucidated. A simple ultra-performance liquid chromatography\u2013tandem mass spectrometry (UPLC-MS\/MS) method was developed and validated for the simultaneous quantitative determination of brucine, strychnine, liquiritin, isoliquiritin, isoliquiritigenin and glycyrrhizic acid concentrations and it was applied to compare the content of different phases and measure the release characteristics of the sedimentary phase in \u201cGlycyrrhiza glabra-Nux vomica\u201d decoction (NGD). The results show that the method\u2019s selectivity, precision (intraday and interday \u2264 2%), matrix effect (101\u2013108%), recovery and stability results were acceptable according to the guidelines. The method is sensitive and reliable. The content determination results show that the most toxic strychnine in the sedimentary phase accounted for 75.70% of the total components. The different components exhibited differential release in different media, and its components were released in the artificial intestinal fluid up to 81.02% in 12 h. Several components conformed to the primary kinetic model and the Ritger\u2013Peppas model, and the most toxic compound exhibited slow release, thus conforming to the Ritger\u2013Peppas model. This study provides a standard of reference for studies investigating reduction in toxicity of the combination of Glycyrrhiza glabra (Glycyrrhiza glabra L.) and Nux vomica (Strychnos nux-vomica L.).<\/jats:p>","DOI":"10.3390\/molecules27196180","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T05:33:14Z","timestamp":1663824794000},"page":"6180","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Content Determination and Release Characteristics of Six Components in the Different Phases of \u201cGlycyrrhizaglabra-Nux vomica\u201d Decoction by UPLC-MS\/MS"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1218-679X","authenticated-orcid":false,"given":"Yuyan","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Shao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Harbin University of Commerce, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuncheng","family":"Li","sequence":"additional","affiliation":[{"name":"Daxing\u2019anling Vocational College, Daxing\u2019anling 165000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zejun","family":"Meng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayu","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaowa","family":"L\u00fc","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s13020-021-00472-9","article-title":"The toxicology and detoxification of Aconitum: Traditional and modern views","volume":"16","author":"Chan","year":"2021","journal-title":"Chin. 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