{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:15:27Z","timestamp":1789708527688,"version":"4.0.1"},"reference-count":35,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:00:00Z","timestamp":1664150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunhe Talent Project"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Due to the decreasing self-repairing ability, elder people are easier to form chronic wounds and suffer from slow and difficult wound healing. It is desirable to develop a novel wound dressing that can accelerate chronic wound healing in elderly subjects to decrease the pain of patients and save medical resources. In this work, Heparin and basic fibroblast growth factor(bFGF) were dissolved in the mixing solution of 4-arm acrylated polyethylene glycol and dithiothreitol to form hydrogel dressing in vitro at room temperature without any catalysts, which is convenient and easy to handle in clinic application. In vitro re-lease test shows the bFGF could be continuously released for at least 7 days, whereas the dressing surface integrity maintained for 3 days degradation in PBS solution. Three groups of treatments including bFGF-Gel, bFGF-Sol and control without any treatment were applied on the full-thickness wound on the 22 months old mice back. The wound closure rate and histological and immunohistochemical staining all illustrated that bFGF-Gel displayed a better wound healing effect than the other two groups. Thus, as-prepared hydrogel dressing seems supe-rior to current clinical treatment and more effective in elderly subjects, which shows promising potential to be applied in the clinic.<\/jats:p>","DOI":"10.3390\/molecules27196361","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T03:30:37Z","timestamp":1664335837000},"page":"6361","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Hydrogel Dressing Containing Basic Fibroblast Growth Factor Accelerating Chronic Wound Healing in Aged Mouse Model"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7402-9012","authenticated-orcid":false,"given":"Yonghao","family":"Xiao","sequence":"first","affiliation":[{"name":"School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongheng","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengguo","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.cej.2019.01.028","article-title":"Degradable conductive injectable hydrogels as novel antibacterial, anti-oxidant wound dressings for wound healing","volume":"362","author":"Qu","year":"2019","journal-title":"Chem. Eng. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.biomaterials.2018.08.044","article-title":"Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing","volume":"183","author":"Qu","year":"2018","journal-title":"Biomaterials"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.eurpolymj.2018.12.019","article-title":"The production and application of hydrogels for wound management: A review","volume":"111","author":"Gupta","year":"2019","journal-title":"Eur. Polym. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.jdermsci.2013.07.008","article-title":"Current understanding of molecular and cellular mechanisms in fibroplasia and angiogenesis during acute wound healing","volume":"72","author":"Greaves","year":"2013","journal-title":"J. Dermatol. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.jconrel.2020.09.039","article-title":"Chronic wounds: Current status, available strategies and emerging therapeutic solutions","volume":"328","author":"Igartua","year":"2020","journal-title":"J. Control. Release"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1007\/s10856-019-6319-6","article-title":"A review of accelerated wound healing approaches: Biomaterial- assisted tissue remodeling","volume":"30","author":"Nour","year":"2019","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.actbio.2017.11.045","article-title":"Effects of structurally stabilized EGF and bFGF on wound healing in type I and type II diabetic mice","volume":"66","author":"Choi","year":"2018","journal-title":"Acta Biomater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.jallcom.2007.05.020","article-title":"Improved wound healing in pressure-induced decubitus ulcer with controlled release of basic fibroblast growth factor","volume":"459","author":"Jiang","year":"2008","journal-title":"J. Alloys Compd."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e117","DOI":"10.1016\/j.jaad.2011.06.028","article-title":"The local treatment and available dressings designed for chronic wounds","volume":"68","author":"Czemplik","year":"2013","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Alven, S., Khwaza, V., Oyedeji, O.O., and Aderibigbe, B.A. (2021). Polymer-Based Scaffolds Loaded with Aloe vera Extract for the Treatment of Wounds. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13070961"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"18710","DOI":"10.1021\/acsami.6b06047","article-title":"Comparative Study of Heparin-Poloxamer Hydrogel Modified bFGF and aFGF for in Vivo Wound Healing Efficiency","volume":"8","author":"Wu","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.eurpolymj.2017.12.046","article-title":"Hydrogel wound dressings for bioactive treatment of acute and chronic wounds","volume":"100","author":"Koehler","year":"2018","journal-title":"Eur. Polym. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"111169","DOI":"10.1016\/j.msec.2020.111169","article-title":"In situ hydrogel dressing loaded with heparin and basic fibroblast growth factor for accelerating wound healing in rat","volume":"116","author":"Peng","year":"2020","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"128144","DOI":"10.1016\/j.cej.2020.128144","article-title":"Electrospun polyvinylpyrrolidone (PVP) hydrogels containing hydroxycinnamic acid derivatives as potential wound dressings","volume":"409","author":"Contardi","year":"2021","journal-title":"Chem. Eng. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"109683","DOI":"10.1016\/j.matdes.2021.109683","article-title":"Core-shell chitosan microsphere with antimicrobial and vascularized functions for promoting skin wound healing","volume":"204","author":"Li","year":"2021","journal-title":"Mater. Des."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bayer, I.S. (2022). Advances in Fibrin-Based Materials in Wound Repair: A Review. Molecules, 27.","DOI":"10.3390\/molecules27144504"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.biomaterials.2017.06.020","article-title":"Polysaccharide matrices used in 3D in vitro cell culture systems","volume":"141","author":"Diekjurgen","year":"2017","journal-title":"Biomaterials"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1039\/b811392k","article-title":"Synthetic polymer scaffolds for tissue engineering","volume":"38","author":"Place","year":"2009","journal-title":"Chem. Soc. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Bayer, I.S. (2021). A Review of Sustained Drug Release Studies from Nanofiber Hydrogels. Biomedicines, 9.","DOI":"10.3390\/biomedicines9111612"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"43472","DOI":"10.1002\/app.43472","article-title":"In vitro and in vivo investigational studies of a nanocomposite-hydrogel-based dressing with a silver-coated chitosan wafer for full-thickness skin wounds","volume":"133","author":"Jaiswal","year":"2016","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1021\/acs.langmuir.8b01834","article-title":"An Antifouling Hydrogel Containing Silver Nanoparticles for Modulating the Therapeutic Immune Response in Chronic Wound Healing","volume":"35","author":"Shi","year":"2019","journal-title":"Langmuir"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e2000198","DOI":"10.1002\/adhm.202000198","article-title":"Absorbable Thioether Grafted Hyaluronic Acid Nanofibrous Hydrogel for Synergistic Modulation of Inflammation Microenvironment to Accelerate Chronic Diabetic Wound Healing","volume":"9","author":"Liu","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"ref_23","first-page":"3962","article-title":"Bioactive skin-mimicking hydrogel band-aids for diabetic wound healing and infectious skin incision treatment","volume":"6","author":"Yang","year":"2021","journal-title":"Bioact. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1039\/C9TB02331C","article-title":"Silver crosslinked injectable bFGF-eluting supramolecular hydrogels speed up infected wound healing","volume":"8","author":"Xuan","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"16054","DOI":"10.1021\/acsami.7b04801","article-title":"Multifunctional Hydrogels Prepared by Dual Ion Cross-Linking for Chronic Wound Healing","volume":"9","author":"Li","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"12687","DOI":"10.1021\/acsnano.1c04206","article-title":"Functional Hydrogels as Wound Dressing to Enhance Wound Healing","volume":"15","author":"Liang","year":"2021","journal-title":"ACS Nano"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3261","DOI":"10.1016\/j.actbio.2012.05.001","article-title":"Rapidly curable chitosan-PEG hydrogels as tissue adhesives for hemostasis and wound healing","volume":"8","author":"Lih","year":"2012","journal-title":"Acta Biomater."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"19165","DOI":"10.1038\/s41598-019-55214-7","article-title":"PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing","volume":"9","author":"Vijayan","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ma, X., Wang, M., Ran, Y., Wu, Y., Wang, J., Gao, F., Liu, Z., Xi, J., Ye, L., and Feng, Z. (2022). Design and Fabrication of Polymeric Hydrogel Carrier for Nerve Repair. Polymers, 14.","DOI":"10.3390\/polym14081549"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1021\/acsabm.0c01225","article-title":"Hydrogel Complex Electrospun Scaffolds and Their Multiple Functions in In Situ Vascular Tissue Engineering","volume":"4","author":"Geng","year":"2021","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.ijbiomac.2019.04.065","article-title":"Wound dressing properties of cationized cotton fabric treated with carrageenan\/cyclodextrin hydrogel loaded with honey bee propolis extract","volume":"133","author":"Sharaf","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"516","DOI":"10.3389\/fbioe.2020.00516","article-title":"Functional Biomaterials for Treatment of Chronic Wound","volume":"8","author":"Zhang","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4439","DOI":"10.1016\/j.biomaterials.2013.02.065","article-title":"Macrophage functional polarization (M1\/M2) in response to varying fiber and pore dimensions of electrospun scaffolds","volume":"34","author":"Garg","year":"2013","journal-title":"Biomaterials"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.lfs.2015.10.025","article-title":"Men and mice: Relating their ages","volume":"152","author":"Dutta","year":"2016","journal-title":"Life Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"117242","DOI":"10.1016\/j.lfs.2019.117242","article-title":"Correlation between mouse age and human age in anti-tumor research: Significance and method establishment","volume":"242","author":"Wang","year":"2020","journal-title":"Life Sci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/19\/6361\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:40:03Z","timestamp":1760143203000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/19\/6361"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,26]]},"references-count":35,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["molecules27196361"],"URL":"https:\/\/doi.org\/10.3390\/molecules27196361","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,26]]}}}