{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:40:53Z","timestamp":1762069253859,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T00:00:00Z","timestamp":1664496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["31901920"],"award-info":[{"award-number":["31901920"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Despite an outstanding agent for control of Lepidoptera, the diamide insecticide cyclaniliprole (CYCP) is a suspected carcinogen. In the present study, an analytical method was developed for the determination of CYCP in six fruits and vegetables (apple, grape, peach, bell pepper, lettuce, and tomato) using ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry. Sample preparation was carried out by the acetonitrile-salting-out extraction followed by simple and fast cleanup of disposable pipette extraction tip containing styrene divinyl benzene and\/or graphitized carbon black. Satisfactory linearity (r &gt; 0.99) was obtained in the calibration range of 0.001\u20131 \u00b5g mL\u22121. Matrix effects decreased from \u22129.9\u2013\u221217.9% to \u22121.0\u2013\u22127.6% after the cleanup. The recoveries of CYCP at three spike levels (0.01, 0.1, and 1 mg kg\u22121) from different matrices were between 75.7% and 111.5%, with the intra-day (n = 5) and inter-day (n = 15) relative standard deviations lower than 12.1%. The limit of quantification was 0.01 mg kg\u22121. The developed method provides a good reference for routine monitoring of CYCP in these fruits and vegetables.<\/jats:p>","DOI":"10.3390\/molecules27196464","type":"journal-article","created":{"date-parts":[[2022,10,9]],"date-time":"2022-10-09T04:00:02Z","timestamp":1665288002000},"page":"6464","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Determination of Cyclaniliprole in Fruits and Vegetables Using Disposable Pipette Extraction Cleanup and Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry"],"prefix":"10.3390","volume":"27","author":[{"given":"Zhou","family":"Lu","sequence":"first","affiliation":[{"name":"Center of Quality Standard and Testing Technology for Agro-Products, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Plant Protection, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiqian","family":"Yue","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiming","family":"Ren","sequence":"additional","affiliation":[{"name":"Center of Quality Standard and Testing Technology for Agro-Products, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhong","family":"Wang","sequence":"additional","affiliation":[{"name":"Center of Quality Standard and Testing Technology for Agro-Products, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueru","family":"Li","sequence":"additional","affiliation":[{"name":"Center of Quality Standard and Testing Technology for Agro-Products, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,30]]},"reference":[{"key":"ref_1","unstructured":"(2022, May 01). IUPAC Pesticide Properties Database. Available online: http:\/\/sitem.herts.ac.uk\/aeru\/iupac\/Reports\/2632.htm."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.pestbp.2005.07.005","article-title":"Anthranilic diamides: A new class of insecticides with a novel mode of action, ryanodine receptor activation","volume":"84","author":"Cordova","year":"2006","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hamamoto, T. (2016, January 25\u201330). Chemical Structure Advantage of Cyclaniliprole Against the Diamides Resistant Diamondback Moth, Plutella Xylostella. Proceedings of the International Congress of Entomology, Orlando, FL, USA.","DOI":"10.1603\/ICE.2016.112450"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"105266","DOI":"10.1016\/j.cropro.2020.105266","article-title":"Baseline susceptibility and frequency of resistance to diamide insecticides in Helicoverpa armigera (Lepidoptera: Noctuidae) populations in Brazil","volume":"137","author":"Pereira","year":"2020","journal-title":"Crop Prot."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.cropro.2017.07.006","article-title":"Resistance monitoring of Chilo suppressalis (Walker) (Lepidoptera: Crambidae) to chlorantraniliprole in eight field populations from east and central China","volume":"100","author":"Lu","year":"2017","journal-title":"Crop Prot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1603\/EC12059","article-title":"High Levels of Resistance to Chlorantraniliprole Evolved in Field Populations of Plutella xylostella","volume":"105","author":"Wang","year":"2012","journal-title":"J. Econ. Entomol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1017\/S0021859605005708","article-title":"Crop losses to pests","volume":"144","author":"Oerke","year":"2006","journal-title":"J. Agric. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA) (2016). Peer review of the pesticide risk assessment of the active substance cyclaniliprole. EFSA J., 14, 4452.","DOI":"10.2903\/j.efsa.2016.4452"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1093\/jaoac\/86.2.412","article-title":"Fast and easy multiresidue method employing acetonitrile extraction\/partitioning and \u201cdispersive solid-phase extraction\u201d for the determination of pesticide residues in produce","volume":"86","author":"Anastassiades","year":"2003","journal-title":"J. AOAC Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1093\/jaoac\/88.2.615","article-title":"Use of buffering and other means to improve results of problematic pesticides in a fast and easy method for residue analysis of fruits and vegetables","volume":"88","author":"Lehotay","year":"2005","journal-title":"J. AOAC Int."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Ohkawa, H., Miyagawa, H., and Lee, P.W. (2007). Recent Developments in QuEChERS Methodology for Pesticide Multiresidue Analysis. Pesticide Chemistry: Crop Protection, Public Health, Environmental Safety, Wiley.","DOI":"10.1002\/9783527611249"},{"key":"ref_12","unstructured":"Association of Official Analytical Chemists (AOAC) (2007). AOAC Official Method 2007.01 Pesticide Residues in Foods by Acetonitrile Extraction and Partitioning with Magnesium Sulfate. J. AOAC Int., 90, 485\u2013520."},{"key":"ref_13","unstructured":"(2008). Foods of Plant Origin\u2212Determination of Pesticide Residues Using GC-MS and\/or LC-MS\/MS following Acetonitrile Extraction and Partitioning and Clean-Up by Dispersive SPE\u2212QuEChERS-Method (Standard No. EN 15662:2008)."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"107552","DOI":"10.1016\/j.foodcont.2020.107552","article-title":"Simultaneous determination of multi-pesticide residues in fish and shrimp using dispersive-solid phase extraction with liquid chromatography-tandem mass spectrometry","volume":"120","author":"Shin","year":"2020","journal-title":"Food Control"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"142015","DOI":"10.1016\/j.scitotenv.2020.142015","article-title":"Optimization and validation of a QuEChERS-based method for the simultaneous environmental monitoring of 218 pesticide residues in clay loam soil","volume":"753","author":"Luzardo","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.foodchem.2019.01.003","article-title":"Determination of neonicotinoids and 199 other pesticide residues in honey by liquid and gas chromatography coupled with tandem mass spectrometry","volume":"282","author":"Gawel","year":"2019","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1080\/03067319.2019.1586892","article-title":"A comparative study of biochar, multiwalled carbon nanotubes and graphitized carbon black as QuEChERS absorbents for the rapid determination of six triazole fungicides by UPLC-MS\/MS","volume":"99","author":"Cao","year":"2019","journal-title":"Int. J. Environ. Anal. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.foodchem.2018.10.051","article-title":"Development of a modified QuEChERS method based on magnetic multiwalled carbon nanotubes for the simultaneous determination of veterinary drugs, pesticides and mycotoxins in eggs by UPLC-MS\/MS","volume":"276","author":"Xu","year":"2019","journal-title":"Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1002\/jssc.201500932","article-title":"Disposable pipette tips extraction: Fundamentals, applications and state of the art","volume":"39","author":"Bordin","year":"2016","journal-title":"J. Sep. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"461004","DOI":"10.1016\/j.chroma.2020.461004","article-title":"Residue analysis of fungicides fenpicoxamid, isofetamid, and mandestrobin in cereals using zirconium oxide disposable pipette extraction clean-up and ultrahigh-performance liquid chromatography-tandem mass spectrometry","volume":"1620","author":"Lu","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.foodchem.2018.04.076","article-title":"Pipette-tip solid-phase extraction using poly(1-vinylimidazole-co-trimethylolpropane trimethacrylate) as a new molecularly imprinted polymer in the determination of avermectins and milbemycins in fruit juice and water samples","volume":"262","author":"Teixeira","year":"2018","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.jchromb.2018.03.026","article-title":"Analysis of 10 \u03b2-agonists in pork meat using automated dispersive pipette extraction and LC-MS\/MS","volume":"1084","author":"Mastrianni","year":"2018","journal-title":"J. Chromatogr. B"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4788","DOI":"10.1021\/jf300234d","article-title":"Multiresidue Analysis of 58 Pesticides in Bean Products by Disposable Pipet Extraction (DPX) Cleanup and Gas Chromatography\u2013Mass Spectrometry Determination","volume":"60","author":"Li","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1016\/j.chroma.2010.01.047","article-title":"Disposable pipette extraction for the analysis of pesticides in fruit and vegetables using gas chromatography\/mass spectrometry","volume":"1217","author":"Guan","year":"2010","journal-title":"J. Chromatogr. A"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.chroma.2006.12.079","article-title":"Application of liquid chromatography\u2013electrospray ionisation mass spectrometry for determination of dietary folates: Effects of buffer nature and mobile phase composition on sensitivity and selectivity","volume":"1143","author":"Patring","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.jchromb.2005.03.041","article-title":"The effect of the mobile phase additives on sensitivity in the analysis of peptides and proteins by high-performance liquid chromatography\u2013electrospray mass spectrometry","volume":"825","author":"Garcia","year":"2005","journal-title":"J. Chromatogr. B"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0021-9673(99)00832-8","article-title":"Solid-phase extraction: Method development, sorbents, and coupling with liquid chromatography","volume":"856","author":"Hennion","year":"1999","journal-title":"J. Chromatogr. A"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2019.02.036","article-title":"QuEChERS-Fundamentals, relevant improvements, applications and future trends","volume":"1070","author":"Perestrelo","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"ref_29","first-page":"30","article-title":"The QuEChERS revolution","volume":"13","author":"Lehotay","year":"2010","journal-title":"LCGC Asia Pac."},{"key":"ref_30","unstructured":"European Commission SANTE\/11312\/2022 (2022, May 01). Guidance Document on Analytical Quality Control and Method Validation Procedures for Pesticide Residues and Analysis in Food and Feed. Available online: https:\/\/ec.europa.eu\/food\/system\/files\/2022-02\/pesticides_mrl_guidelines_wrkdoc_2021-11312.pdf."},{"key":"ref_31","unstructured":"(2022, May 01). China Pesticide Information Network. Available online: http:\/\/www.chinapesticide.org.cn\/hysj\/index.jhtml."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1002\/mas.20097","article-title":"Matrix effects in quantitative pesticide analysis using liquid chromatography-mass spectrometry","volume":"25","author":"Niessen","year":"2006","journal-title":"Mass Spectrom. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1021\/ac202661j","article-title":"Reduction of matrix effects in liquid chromatography-electrospray ionization-mass spectrometry by dilution of the sample extracts: How much dilution is needed?","volume":"84","author":"Stahnke","year":"2012","journal-title":"Anal. Chem."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/19\/6464\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:44:54Z","timestamp":1760143494000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/19\/6464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,30]]},"references-count":33,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["molecules27196464"],"URL":"https:\/\/doi.org\/10.3390\/molecules27196464","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2022,9,30]]}}}