{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:58:25Z","timestamp":1787029105095,"version":"build-2736575974"},"reference-count":50,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21772229"],"award-info":[{"award-number":["21772229"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In order to improve the antifungal activity of new butenolides containing oxime ether moiety, a series of new butenolide compounds containing methoxyacrylate scaffold were designed and synthesized, based on the previous reports. Their structures were characterized by 1H NMR, 13C NMR, HR-MS spectra, and X-ray diffraction analysis. The in vitro antifungal activities were evaluated by the mycelium growth rate method. The results showed that the inhibitory activities of these new compounds against Sclerotinia sclerotiorum were significantly improved, in comparison with that of the lead compound 3\u20138; the EC50 values of V-6 and VI-7 against S. sclerotiorum were 1.51 and 1.81 mg\/L, nearly seven times that of 3\u20138 (EC50 10.62 mg\/L). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observation indicated that compound VI-3 had a significant impact on the structure and function of the hyphal cell of S. sclerotiorum mycelium and the positive control trifloxystrobin. Molecular simulation docking results indicated that the introduction of methoxyacrylate scaffold is beneficial to improving the antifungal activity of these compounds against S. sclerotiorum, which can be used as the lead for further structure optimization.<\/jats:p>","DOI":"10.3390\/molecules27196541","type":"journal-article","created":{"date-parts":[[2022,10,9]],"date-time":"2022-10-09T23:31:43Z","timestamp":1665358303000},"page":"6541","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Synthesis and Antifungal Activity of New butenolide Containing Methoxyacrylate Scaffold"],"prefix":"10.3390","volume":"27","author":[{"given":"Qian","family":"Zhang","sequence":"first","affiliation":[{"name":"Innovation Center of Pesticide Research, Department of Applied Chemistry, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihao","family":"Li","sequence":"additional","affiliation":[{"name":"Innovation Center of Pesticide Research, Department of Applied Chemistry, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Innovation Center of Pesticide Research, Department of Applied Chemistry, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leichuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Innovation Center of Pesticide Research, Department of Applied Chemistry, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyun","family":"Ma","sequence":"additional","affiliation":[{"name":"Innovation Center of Pesticide Research, Department of Applied Chemistry, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3852-0672","authenticated-orcid":false,"given":"Mingan","family":"Wang","sequence":"additional","affiliation":[{"name":"Innovation Center of Pesticide Research, Department of Applied Chemistry, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10761","DOI":"10.1021\/acs.jafc.1c01460","article-title":"In Silico resources of drug-likeness as a mirror: What are we lacking in pesticide-likeness?","volume":"69","author":"Ouyang","year":"2021","journal-title":"J. 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