{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:20:45Z","timestamp":1791404445995,"version":"4.3.3"},"reference-count":47,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T00:00:00Z","timestamp":1665532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The antifungal drugs currently available and mostly used for the treatment of candidiasis exhibit the phenomena of toxicity and increasing resistance. In this context, plant materials might represent promising sources of antifungal agents. The aim of this study is to evaluate for the first time the chemical content of the volatile fractions (VFs) along with the antifungal and anti-biofilm of Convolvulus althaeoides L. roots. The chemical composition was determined by gas chromatography coupled to a flame ionization detector and mass spectrometry. In total, 73 and 86 chemical compounds were detected in the n-hexane (VF1) and chloroform (VF2) fractions, respectively. Analysis revealed the presence of four main compounds: n-hexadecenoic acid (29.77%), 4-vinyl guaiacol (12.2%), bis(2-ethylhexyl)-adipate (9.69%) and eicosane (3.98%) in the VF extracted by hexane (VF1). n-hexadecenoic acid (34.04%), benzyl alcohol (7.86%) and linoleic acid (7.30%) were the main compounds found in the VF extracted with chloroform (VF2). The antifungal minimum inhibitory concentrations (MICs) of the obtained fractions against Candida albicans, Candida glabrata and Candida tropicalis were determined by the micro-dilution technique and values against Candida spp. ranged from 0.87 to 3.5 mg\/mL. The biofilm inhibitory concentrations (IBF) and sustained inhibition (BSI) assays on C. albicans, C. glabrata and C. tropicalis were also investigated. The VFs inhibited biofilm formation up to 0.87 mg\/mL for C. albicans, up to 1.75 mg\/mL against C. glabrata and up to 0.87 mg\/mL against C. tropicalis. The obtained results highlighted the synergistic mechanism of the detected molecules in the prevention of candidosic biofilm formation.<\/jats:p>","DOI":"10.3390\/molecules27206834","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T22:45:29Z","timestamp":1665614729000},"page":"6834","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Chemical Composition, Antifungal and Anti-Biofilm Activities of Volatile Fractions of Convolvulus althaeoides L. Roots from Tunisia"],"prefix":"10.3390","volume":"27","author":[{"given":"Soukaina","family":"Hrichi","sequence":"first","affiliation":[{"name":"Laboratory of Physico-Chemistry of Materials, Faculty of Sciences of Monastir, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raja","family":"Cha\u00e2bane-Banaoues","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Mycology (LP3M), Department of Clinical Biology, Faculty of Pharmacy of Monastir, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Alibrando","sequence":"additional","affiliation":[{"name":"Chromaleont s.r.l., c\/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7750-5988","authenticated-orcid":false,"given":"Ammar B.","family":"Altemimi","sequence":"additional","affiliation":[{"name":"Department of Food Science, College of Agriculture, University of Basrah, Basrah 61004, Iraq"},{"name":"College of Medicine, University of Warith Al-Anbiyaa, Karbala 56001, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oussama","family":"Babba","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Mycology (LP3M), Department of Clinical Biology, Faculty of Pharmacy of Monastir, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yassine Oulad El","family":"Majdoub","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habib","family":"Nasri","sequence":"additional","affiliation":[{"name":"Laboratory of Physico-Chemistry of Materials, Faculty of Sciences of Monastir, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8890-675X","authenticated-orcid":false,"given":"Luigi","family":"Mondello","sequence":"additional","affiliation":[{"name":"Chromaleont s.r.l., c\/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98122 Messina, Italy"},{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98122 Messina, Italy"},{"name":"Department of Sciences and Technologies for Human and Environment, University Campus Bio-Medico of Rome, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamouda","family":"Babba","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Mycology (LP3M), Department of Clinical Biology, Faculty of Pharmacy of Monastir, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zine","family":"Mighri","sequence":"additional","affiliation":[{"name":"Laboratory of Physico-Chemistry of Materials, Faculty of Sciences of Monastir, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1296-7633","authenticated-orcid":false,"given":"Francesco","family":"Cacciola","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.3389\/fmicb.2015.01420","article-title":"Potential Use of Phenolic Acids as Anti-Candida Agents: A Review","volume":"6","author":"Teodoro","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_2","first-page":"965","article-title":"Antifungal activity of the alkaloids extracts from aerial parts of Retama monosperma","volume":"7","author":"Fdil","year":"2016","journal-title":"Res. J. Pharm. Biol. Chem. Sci."},{"key":"ref_3","first-page":"111","article-title":"Antibacterial activity of some medicinal plants against carbapenem-resistant Acinetobacter baumannii isolated from patients","volume":"6","author":"Matin","year":"2019","journal-title":"Eur. J. Pharm. Med. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3025","DOI":"10.15376\/biores.17.2.3025-3041","article-title":"Application of Synthetic Acyl Glucopyranosides for White-rot and Brown-rot Fungal Decay Resistance in Aspen and Pine Wood","volume":"17","author":"Matin","year":"2022","journal-title":"Bioresources"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jphotobiol.2016.12.025","article-title":"Evaluation of the catalytic, antibacterial and anti-biofilm activities of the Convolvulus arvensis extract functionalized silver nanoparticles","volume":"167","author":"Hamedi","year":"2017","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_6","unstructured":"Belwal, T., Nabavi, S.M., Nabavi, S.F., Dehpour, A.R., and Shirooie, S. (2021). Chapter 3.2.19\u2014Convolvulus prostratus. Naturally Occurring Chemicals Against Alzheimer\u2019s Disease, Academic Press."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5694305","DOI":"10.1155\/2017\/5694305","article-title":"Antibacterial, Antioxidant Activity of Ethanolic Plant Extracts of Some Convolvulus Species and Their DART-ToF-MS Profiling","volume":"2017","author":"Aqel","year":"2017","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1016\/j.biopha.2018.11.046","article-title":"Protective effect of Convolvulus pluricaulis against neuroinflammation associated depressive behavior induced by chronic unpredictable mild stress in rat","volume":"109","author":"Gupta","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"170","DOI":"10.3390\/antiox4010170","article-title":"The Effect of Convolvulus arvensis Dried Extract as a Potential Antioxidant in Food Models","volume":"4","author":"Azman","year":"2015","journal-title":"Antioxidants"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5651","DOI":"10.1016\/j.arabjc.2020.04.006","article-title":"Effect of seasonal variation on the chemical composition and antioxidant and antifungal activities of Convolvulus althaeoides L. leaf extracts","volume":"13","author":"Hrichi","year":"2020","journal-title":"Arab. J. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1080\/14786419.2013.879476","article-title":"Chemical composition, antibacterial and cytotoxic activities of the essential oil from the flowers of Tunisian Convolvulus althaeoides L","volume":"28","author":"Hassine","year":"2014","journal-title":"Nat. Prod. Res."},{"key":"ref_12","first-page":"1965","article-title":"A Novel Acylated Anthocyanin with a Linear Trisaccharide from Flowers of Convolvulus althaeoides","volume":"10","author":"Cabrita","year":"2015","journal-title":"Nat. Prod. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.foodchem.2018.04.012","article-title":"Antioxidant activity, total phenolics and flavonoids contents: Should we ban in vitro screening methods?","volume":"264","author":"Granato","year":"2018","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1128\/CMR.00029-06","article-title":"Epidemiology of Invasive Candidiasis: A Persistent Public Health Problem","volume":"20","author":"Pfaller","year":"2007","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Bhattacharya, S., Sae-Tia, S., and Fries, B.C. (2020). Candidiasis and Mechanisms of Antifungal Resistance. Antibiotics, 9.","DOI":"10.3390\/antibiotics9060312"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"456878","DOI":"10.1155\/2014\/456878","article-title":"Virulence Factors Contributing to Pathogenicity of Candida tropicalis and Its Antifungal Susceptibility Profile","volume":"2014","author":"Deorukhkar","year":"2014","journal-title":"Int. J. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1128\/EC.4.4.633-638.2005","article-title":"Candida Biofilms: An Update","volume":"4","author":"Ramage","year":"2005","journal-title":"Eukaryot. Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.indcrop.2007.02.004","article-title":"In vitro susceptibility of some species of yeasts and filamentous fungi to essential oils of Salvia officinalis","volume":"26","author":"Pinto","year":"2007","journal-title":"Ind. Crops Prod."},{"key":"ref_19","first-page":"292","article-title":"Chemical composition and antifungal properties of commercial essential oils against the maize phytopathogenic fungus Fusarium verticillioides","volume":"53","author":"Brito","year":"2021","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_20","first-page":"5","article-title":"Phytochemical analysis in economically important Ficus Benghalensis L. and Ficus Krishnae C.DC. using GC-MS","volume":"10","author":"Karthikeyan","year":"2019","journal-title":"Int. J. Pharma. Bio. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1080\/01490451.2011.598407","article-title":"Microbial Activity and 13C\/12C Ratio as Evidence of N-Hexadecane and N-Hexadecanoic Acid Biodegradation in Agricultural and Forest Soils","volume":"29","author":"Zyakun","year":"2012","journal-title":"Geomicrobiol. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.ijbiomac.2014.12.049","article-title":"Structural characterization of lignin: A potential source of antioxidants guaiacol and 4-vinylguaiacol","volume":"75","author":"Azadfar","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"111620","DOI":"10.1016\/j.ecoenv.2020.111620","article-title":"Di(2-ethylhexyl) adipate plasticizer triggers hepatic, brain, and cardiac injury in rats: Mitigating effect of Peganum harmala oil","volume":"208","author":"Behairy","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"357","DOI":"10.6026\/97320630009357","article-title":"Heparin-benzyl alcohol enhancement of biofilms formation and antifungal susceptibility of vaginal Candida species isolated from pregnant and nonpregnant Saudi women","volume":"9","year":"2013","journal-title":"Bioinformation"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Qiu, M., Wang, Y., Sun, L., Deng, Q., and Zhao, J. (2021). Fatty Acids and Oxylipins as Antifungal and Anti-Mycotoxin Agents in Food: A Review. Toxins, 13.","DOI":"10.3390\/toxins13120852"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3616","DOI":"10.1016\/j.sjbs.2021.03.040","article-title":"Palmitic acid mediated change of rhizosphere and alleviation of Fusarium wilt disease in watermelon","volume":"28","author":"Ma","year":"2021","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s13568-017-0351-z","article-title":"Extraction and identification of bioactive compounds (eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf","volume":"7","author":"Ahsan","year":"2017","journal-title":"AMB Express"},{"key":"ref_28","first-page":"415","article-title":"In vitro antifungal activity of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens","volume":"29","author":"Morcia","year":"2012","journal-title":"Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.gaost.2021.03.002","article-title":"Vanillin and its derivatives, potential promising antifungal agents, inhibit Aspergillus flavus spores via destroying the integrity of cell membrane rather than cell wall","volume":"4","author":"Li","year":"2021","journal-title":"Grain Oil Sci. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"762844","DOI":"10.3389\/fmicb.2021.762844","article-title":"The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production in Fusarium graminearum","volume":"12","author":"Gao","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_31","first-page":"2286","article-title":"19. Antibacterial and antifungal activities of extracts of Convolvulus leiocalycinus and Haloxylon griffithii of Balochistan, Pakistan","volume":"8","author":"Baqi","year":"2019","journal-title":"Pure Appl. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.ijpharm.2014.08.028","article-title":"Shankhapushpi (Convolvulus pluricaulis Choisy): Validation of the Ayurvedic therapeutic claims through contemporary studies","volume":"8","author":"Amin","year":"2014","journal-title":"Int. J. Green Pharm."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"111720","DOI":"10.1016\/j.indcrop.2019.111720","article-title":"Combined effect of Thymus capitatus and Cinnamomum verum essential oils with conventional drugs against Candida albicans biofilm formation and elucidation of the molecular mechanism of action","volume":"140","author":"Essid","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.micinf.2016.01.002","article-title":"Candida albicans biofilms: Development, regulation, and molecular mechanisms","volume":"18","author":"Gulati","year":"2016","journal-title":"Microbes Infect."},{"key":"ref_35","unstructured":"Somma, A.D., Moretta, A., Can\u00e8, C., Cirillo, A., and Duilio, A. (2020). Inhibition of Bacterial Biofilm Formation, IntechOpen."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Tits, J., Cammue, B.P.A., and Thevissen, K. (2020). Combination Therapy to Treat Fungal Biofilm-Based Infections. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228873"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"153710","DOI":"10.1016\/j.phymed.2021.153710","article-title":"Antibiofilm activities of fatty acids including myristoleic acid against Cutibacterium acnes via reduced cell hydrophobicity","volume":"91","author":"Kim","year":"2021","journal-title":"Phytomedicine"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1080\/08927014.2012.749398","article-title":"Terpenoids of plant origin inhibit morphogenesis, adhesion, and biofilm formation by Candida albicans","volume":"29","author":"Raut","year":"2013","journal-title":"Biofouling"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1039\/C9NP00011A","article-title":"Synergy and antagonism in natural product extracts: When 1 + 1 does not equal 2","volume":"36","author":"Caesar","year":"2019","journal-title":"Nat. Prod. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Hrichi, S., Chaabane-Banaoues, R., Bayar, S., Flamini, G., Oulad El Majdoub, Y., Mangraviti, D., Mondello, L., El Mzoughi, R., Babba, H., and Mighri, Z. (2020). Botanical and Genetic Identification Followed by Investigation of Chemical Composition and Biological Activities on the Scabiosa atropurpurea L. Stem from Tunisian Flora. Molecules, 25.","DOI":"10.3390\/molecules25215032"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"112924","DOI":"10.1016\/j.lwt.2021.112924","article-title":"Action mode of cuminaldehyde against Staphylococcus aureus and its application in sauced beef","volume":"155","author":"Li","year":"2022","journal-title":"LWT"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1128\/AEM.02515-15","article-title":"Pentanol and Benzyl Alcohol Attack Bacterial Surface Structures Differently","volume":"82","author":"Yano","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Guimar\u00e3es, A., and Ven\u00e2ncio, A. (2022). The Potential of Fatty Acids and Their Derivatives as Antifungal Agents: A Review. Toxins, 14.","DOI":"10.3390\/toxins14030188"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"864","DOI":"10.3389\/fmicb.2020.00864","article-title":"Palmitic Acid Inhibits the Virulence Factors of Candida tropicalis: Biofilms, Cell Surface Hydrophobicity, Ergosterol Biosynthesis, and Enzymatic Activity","volume":"11","author":"Prasath","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"36133","DOI":"10.1039\/C8RA06413J","article-title":"Inhibition of biofilm formation and quorum sensing mediated phenotypes by berberine in Pseudomonas aeruginosa and Salmonella typhimurium","volume":"8","author":"Aswathanarayan","year":"2018","journal-title":"RSC Adv."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s13568-016-0203-2","article-title":"Mycelial pellet formation by edible ascomycete filamentous fungi, Neurospora intermedia","volume":"6","author":"Nair","year":"2016","journal-title":"AMB Express"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.3389\/fmicb.2020.01027","article-title":"Combination of Antifungal Drugs and Protease Inhibitors Prevent Candida albicans Biofilm Formation and Disrupt Mature Biofilms","volume":"11","author":"Lohse","year":"2020","journal-title":"Front. Microbiol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/20\/6834\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:52:46Z","timestamp":1760143966000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/20\/6834"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,12]]},"references-count":47,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["molecules27206834"],"URL":"https:\/\/doi.org\/10.3390\/molecules27206834","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,12]]}}}