{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T23:41:45Z","timestamp":1788565305749,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T00:00:00Z","timestamp":1665705600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Taif University Researchers Supporting Project","award":["TURSP-2020\/14"],"award-info":[{"award-number":["TURSP-2020\/14"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Plants of the genus Strobilanthes have notable use in folklore medicines as well as being used for pharmacological purposes. The present work explored the biological predispositions of Strobilanthes glutinosus and attempted to accomplish a comprehensive chemical profile through GC-MS of different fractions concerning polarity (chloroform and n-butanol) and LC-ESI-MS of methanolic extract by both positive and negative ionization modes. The biological characteristics such as antioxidant potential were assessed by applying six different methods. The potential for clinically relevant enzyme (\u03b1-amylase, \u03b1-glucosidase, and tyrosinase) inhibition was examined. The DPPH, ABTS, CUPRAC, and FRAP results revealed that the methanol fraction presented efficient results. The phosphomolybdenum assay revealed that the n-hexane fraction showed the most efficient results, while maximum metal chelation potential was observed for the chloroform fraction. The GC-MS profiling of n-butanol and chloroform fractions revealed the existence of several (110) important compounds presenting different classes (fatty acids, phenols, alkanes, monoterpenes, diterpenes, sesquiterpenoids, and sterols), while LC-ESI-MS tentatively identified the presence of 44 clinically important secondary metabolites. The n-hexane fraction exhibited the highest potential against \u03b1-amylase (497.98 mm ACAE\/g extract) and \u03b1-glucosidase (605.85 mm ACAE\/g extract). Significant inhibitory activity against tyrosinase enzyme was displayed by fraction. Six of the prevailing compounds from the GC-MS study (lupeol, beta-amyrin, stigmasterol, gamma sitosterol, 9,12-octadecadienoic acid, and n-hexadecanoic acid) were modelled against \u03b1-glucosidase and \u03b1-amylase enzymes along with a comparison of binding affinity to standard acarbose, while three compounds identified through LC-ESI-MS were docked to the mushroom tyrosinase enzyme and presented with significant biding affinities. Thus, it is assumed that S. glutinosus demonstrated effective antioxidant and enzyme inhibition prospects with effective bioactive molecules, potentially opening the door to a new application in the field of medicine.<\/jats:p>","DOI":"10.3390\/molecules27206885","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T03:43:58Z","timestamp":1665978238000},"page":"6885","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Comprehensive Biological Potential, Phytochemical Profiling Using GC-MS and LC-ESI-MS, and In-Silico Assessment of Strobilanthes glutinosus Nees: An Important Medicinal Plant"],"prefix":"10.3390","volume":"27","author":[{"given":"Marya","family":"Aziz","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeed","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umair","family":"Khurshid","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irfan","family":"Pervaiz","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Chenab, Gujrat 50700, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arslan Hussain","family":"Lodhi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1279-2397","authenticated-orcid":false,"given":"Nasrullah","family":"Jan","sequence":"additional","affiliation":[{"name":"Akson College of Pharmacy, Mirpur University of Science and Technology, Mirpur 10250, Pakistan"},{"name":"Department of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameera","family":"Khurshid","sequence":"additional","affiliation":[{"name":"Bahawalpur College of Pharmacy, Bahawalpur Medical and Dental College, Bahawalpur 63100, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Adeel","family":"Arshad","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences, Lahore College for Women University, Lahore 54000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed M.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Taif University, Taif 21944, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7529-3085","authenticated-orcid":false,"given":"Gaber A. M.","family":"Mersal","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Taif University, Taif 21944, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahaad S.","family":"Alenazi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Medicine, University of Hail, Hail 55473, Saudi Arabia"},{"name":"Medical Education Unit, College of Medicine, University of Hail, Hail 55473, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Awadh Saleh Alamri","sequence":"additional","affiliation":[{"name":"Medical Services, Ministry of Interior-Security Forces Hospital in Najran, Najran 66256, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juwairiya","family":"Butt","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hammad","family":"Saleem","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences (IPS), University of Veterinary and Animal Sciences (UVAS), Lahore 54000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinhom M.","family":"El-Bahy","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Al-Azhar University, Nasr City 11884, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zahra, W., Rai, S.N., Birla, H., Singh, S.S., Rathore, A.S., Dilnashin, H., Keswani, C., and Singh, S.P. 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