{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:36:51Z","timestamp":1791463011913,"version":"4.3.4"},"reference-count":43,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T00:00:00Z","timestamp":1666396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Scientific evidence suggests that quercetin (QUR) has anxiolytic-like effects in experimental animals. However, the mechanism of action responsible for its anxiolytic-like effects is yet to be discovered. The goal of this research is to assess QUR\u2019s anxiolytic effects in mouse models to explicate the possible mechanism of action. After acute intraperitoneal (i.p.) treatment with QUR at a dose of 50 mg\/kg (i.p.), behavioral models of open-field, hole board, swing box, and light\u2013dark tests were performed. QUR was combined with a GABAergic agonist (diazepam) and\/or antagonist (flumazenil) group. Furthermore, in silico analysis was also conducted to observe the interaction of QUR and GABA (\u03b15), GABA (\u03b21), and GABA (\u03b22) receptors. In the experimental animal model, QUR had an anxiolytic-like effect. QUR, when combined with diazepam (2 mg\/kg, i.p.), drastically potentiated an anxiolytic effect of diazepam. QUR is a more highly competitive ligand for the benzodiazepine recognition site that can displace flumazenil (2.5 mg\/kg, i.p.). In all the test models, QUR acted similar to diazepam, with enhanced effects of the standard anxiolytic drug, which were reversed by pre-treatment with flumazenil. QUR showed the best interaction with the GABA (\u03b15) receptor compared to the GABA (\u03b21) and GABA (\u03b22) receptors. In conclusion, QUR may exert an anxiolytic-like effect on mice, probably through the GABA-receptor-interacting pathway.<\/jats:p>","DOI":"10.3390\/molecules27217149","type":"journal-article","created":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T08:19:06Z","timestamp":1666599546000},"page":"7149","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Anxiolytic-like Effect of Quercetin Possibly through GABA Receptor Interaction Pathway: In Vivo and In Silico Studies"],"prefix":"10.3390","volume":"27","author":[{"given":"Md. Shahazul","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4773-8320","authenticated-orcid":false,"given":"Rajib","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taukir","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3235-9117","authenticated-orcid":false,"given":"Md. Mizanur","family":"Rahaman","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2165-9256","authenticated-orcid":false,"given":"Khattab","family":"Al-Khafaji","sequence":"additional","affiliation":[{"name":"College of Dentistry, The University of Mashreq, Baghdad 10022, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4911-4335","authenticated-orcid":false,"given":"Rasel Ahmed","family":"Khan","sequence":"additional","affiliation":[{"name":"Pharmacy Discipline, Life Science School, Khulna University, Khulna 9280, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6094-0243","authenticated-orcid":false,"given":"Chandan","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1148-2944","authenticated-orcid":false,"given":"Mehedi Hasan","family":"Bappi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6976-9139","authenticated-orcid":false,"given":"Edlane Martins","family":"de Andrade","sequence":"additional","affiliation":[{"name":"CECAPE\u2014College of Dentistry, Av. Padre C\u00edcero 3917, S\u00e3o Jos\u00e9 63024-015, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac Moura","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, Regional University of Cariri\u2014URCA, Crato 63105-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6634-4207","authenticated-orcid":false,"given":"Henrique Douglas Melo","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, Regional University of Cariri\u2014URCA, Crato 63105-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1872-2381","authenticated-orcid":false,"given":"Gra\u017cyna","family":"Kowalska","sequence":"additional","affiliation":[{"name":"Department of Tourism and Recreation, University of Life Sciences in Lublin, 15 Akademicka Str., 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3063-8710","authenticated-orcid":false,"given":"Rados\u0142aw","family":"Kowalski","sequence":"additional","affiliation":[{"name":"Department of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, 8 Skromna Str., 20-704 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Asif","family":"Hanif","sequence":"additional","affiliation":[{"name":"Nano and Biomaterials Lab, Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0034-8202","authenticated-orcid":false,"given":"Muhammad Torequl","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.3389\/fpsyt.2018.00064","article-title":"Mental Disorder\u2014The Need for an Accurate Definition","volume":"9","author":"Saraiva","year":"2018","journal-title":"Front. 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