{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T06:17:53Z","timestamp":1783577873814,"version":"3.55.0"},"reference-count":25,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Key Laboratory of Bioactive Substance and Function of Natural Medicines","award":["GTZK202201"],"award-info":[{"award-number":["GTZK202201"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Tripterygium wilfordii Hook. f. is a well-known traditional Chinese medicine used to treat autoimmune diseases. Sesquiterpene pyridine alkaloids (SPAs) are a major class of components found in this herb that have piqued the interest of researchers due to their complex and diverse structures as well as significant biological activities. In this study, ten new SPAs, wilfordatine A\u2013J (1\u201310), were isolated from the roots of T. wilfordii, along with ten known analogues (11\u201320). Their structures were primarily elucidated by extensive 1D and 2D NMR spectroscopic analysis. To search for more immunosuppressive ingredients related to the clinical efficacy of T. wilfordii, the total alkaloids (TA) and compounds 4, 5, and 9\u201316 were tested for their inhibitory effects on nuclear factor-kappa B (NF-\u03baB) pathway in Lipopolysaccharide (LPS) induced HEK293\/NF-\u03baB-Luc cells. Among them, TA, compounds 5, 11, and 16 showed potent immunosuppressive activity, with IC50 values of 7.25 \u03bcg\/mL, 8.75 \u03bcM, 0.74 \u03bcM, and 15.66 \u03bcM, respectively, and no influence on the cell viability at a concentration of 100 \u03bcg\/mL (TA) or 100 \u03bcM (5, 11, and 16). Accordingly, TA, 5, 11, and 16, especially 11, were identified as promising candidates for further investigation into their potential use as immunosuppressive agents.<\/jats:p>","DOI":"10.3390\/molecules27217274","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T07:17:48Z","timestamp":1666768668000},"page":"7274","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Immunosuppressive Sesquiterpene Pyridine Alkaloids from Tripterygium wilfordii Hook. f."],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5753-9571","authenticated-orcid":false,"given":"Yadan","family":"Wang","sequence":"first","affiliation":[{"name":"National Institutes for Food and Drug Control, Beijing 102629, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangong","family":"Yan","sequence":"additional","affiliation":[{"name":"National Institutes for Food and Drug Control, Beijing 102629, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongmou","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Institutes for Food and Drug Control, Beijing 102629, China"},{"name":"School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghui","family":"Chen","sequence":"additional","affiliation":[{"name":"National Institutes for Food and Drug Control, Beijing 102629, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7675-0543","authenticated-orcid":false,"given":"Xianfu","family":"Wu","sequence":"additional","affiliation":[{"name":"National Institutes for Food and Drug Control, Beijing 102629, China"},{"name":"State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangcheng","family":"Ma","sequence":"additional","affiliation":[{"name":"National Institutes for Food and Drug Control, Beijing 102629, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1007\/s11684-018-0649-5","article-title":"Immunoregulatory effects of Tripterygium wilfordii Hook F and its extracts in clinical practice","volume":"13","author":"Luo","year":"2019","journal-title":"Front. 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