{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:18:48Z","timestamp":1790050728004,"version":"4.0.1"},"reference-count":40,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"National Institute for Food and Agriculture of the United States Department of Agriculture","doi-asserted-by":"publisher","award":["NC.X-329-5-20-120-1"],"award-info":[{"award-number":["NC.X-329-5-20-120-1"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Salmonella enteritidis is a foodborne pathogen that causes high morbidity in poultry. Proteomic analysis by liquid chromatography tandem mass spectrometry (LC-MS\/MS) was used to study the effects of Salmonella infection on spleen proteome in broiler chickens. Day-old broilers were assigned to control (CON; n = 60) or Salmonella challenge (CON\u2212SE; n = 60), and gavaged with Tryptic soy agar broth or SE. A subset of chicks was euthanized on D3 and D7 (n = 4\/group\/day) and the spleen was removed, and rapidly frozen, subsequently proteome was measured using label-free LC-MS\/MS. Protein spectra were mapped to Gallus gallus Uniprot database. Differentially abundant proteins (DAP; FDR &lt; 0.05) between days and treatments were identified using ANOVA. Cecal content of Salmonella in CON\u2212SE was 3.37 log10 CFU\/g and CON were negative. Across the 16 samples, 2625 proteins were identified. Proteins that decreased in abundance between days mediated cell cycle progression, while those that increased in abundance function in cytoskeleton and mRNA processing. SE infection caused an increase in proteins that mediated redox homeostasis, lysosomal activities, and energy production, while proteins decreased in abundance-mediated developmental progression. Proteomic signatures of spleen suggest SE infection was metabolically costly, and energy was diverted from normal developmental processes to potentiate disease resistance mechanisms.<\/jats:p>","DOI":"10.3390\/molecules27217277","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T07:17:48Z","timestamp":1666768668000},"page":"7277","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Proteomic Analysis of the Effect of Salmonella Challenge on Broiler Chicken"],"prefix":"10.3390","volume":"27","author":[{"given":"Adedeji","family":"Adetunji","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8835-3550","authenticated-orcid":false,"given":"Theresa","family":"Casey","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0737-4764","authenticated-orcid":false,"given":"Jackeline","family":"Franco","sequence":"additional","affiliation":[{"name":"Purdue Proteomics Facility, Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6365-7868","authenticated-orcid":false,"given":"Devendra","family":"Shah","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yewande","family":"Fasina","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1637\/11590-011817-RegR","article-title":"A Retrospective Study of Salmonella Enteritidis Isolated from Commercial Layer Flocks","volume":"61","author":"Denagamage","year":"2017","journal-title":"Avian Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ehuwa, O., Jaiswal, A.K., and Jaiswal, S. 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