{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:24:07Z","timestamp":1791321847255,"version":"4.3.1"},"reference-count":44,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pasteur Institute, Ministry of Health &amp; Ministry of High Education","award":["GOA4919N"],"award-info":[{"award-number":["GOA4919N"]}]},{"name":"Pasteur Institute, Ministry of Health &amp; Ministry of High Education","award":["GOE7120N"],"award-info":[{"award-number":["GOE7120N"]}]},{"name":"Pasteur Institute, Ministry of Health &amp; Ministry of High Education","award":["GOC2319N"],"award-info":[{"award-number":["GOC2319N"]}]},{"name":"Pasteur Institute, Ministry of Health &amp; Ministry of High Education","award":["PDM\/19\/164"],"award-info":[{"award-number":["PDM\/19\/164"]}]},{"name":"Pasteur Institute, Ministry of Health &amp; Ministry of High Education","award":["12W7822N"],"award-info":[{"award-number":["12W7822N"]}]},{"name":"Scientific Research of Tunisia","award":["GOA4919N"],"award-info":[{"award-number":["GOA4919N"]}]},{"name":"Scientific Research of Tunisia","award":["GOE7120N"],"award-info":[{"award-number":["GOE7120N"]}]},{"name":"Scientific Research of Tunisia","award":["GOC2319N"],"award-info":[{"award-number":["GOC2319N"]}]},{"name":"Scientific Research of Tunisia","award":["PDM\/19\/164"],"award-info":[{"award-number":["PDM\/19\/164"]}]},{"name":"Scientific Research of Tunisia","award":["12W7822N"],"award-info":[{"award-number":["12W7822N"]}]},{"name":"FWO-Vlaanderen","award":["GOA4919N"],"award-info":[{"award-number":["GOA4919N"]}]},{"name":"FWO-Vlaanderen","award":["GOE7120N"],"award-info":[{"award-number":["GOE7120N"]}]},{"name":"FWO-Vlaanderen","award":["GOC2319N"],"award-info":[{"award-number":["GOC2319N"]}]},{"name":"FWO-Vlaanderen","award":["PDM\/19\/164"],"award-info":[{"award-number":["PDM\/19\/164"]}]},{"name":"FWO-Vlaanderen","award":["12W7822N"],"award-info":[{"award-number":["12W7822N"]}]},{"name":"KU Leuven funding","award":["GOA4919N"],"award-info":[{"award-number":["GOA4919N"]}]},{"name":"KU Leuven funding","award":["GOE7120N"],"award-info":[{"award-number":["GOE7120N"]}]},{"name":"KU Leuven funding","award":["GOC2319N"],"award-info":[{"award-number":["GOC2319N"]}]},{"name":"KU Leuven funding","award":["PDM\/19\/164"],"award-info":[{"award-number":["PDM\/19\/164"]}]},{"name":"KU Leuven funding","award":["12W7822N"],"award-info":[{"award-number":["12W7822N"]}]},{"name":"F.W.O. Vlaanderen","award":["GOA4919N"],"award-info":[{"award-number":["GOA4919N"]}]},{"name":"F.W.O. Vlaanderen","award":["GOE7120N"],"award-info":[{"award-number":["GOE7120N"]}]},{"name":"F.W.O. Vlaanderen","award":["GOC2319N"],"award-info":[{"award-number":["GOC2319N"]}]},{"name":"F.W.O. Vlaanderen","award":["PDM\/19\/164"],"award-info":[{"award-number":["PDM\/19\/164"]}]},{"name":"F.W.O. Vlaanderen","award":["12W7822N"],"award-info":[{"award-number":["12W7822N"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Scorpion venom is a rich source of promising therapeutic compounds, such as highly selective ion channel ligands with potent pharmacological effects. Bot33 is a new short polypeptide of 38 amino acid residues with six cysteines purified from the venom of the Buthus occitanus tunetanus scorpion. Bot33 has revealed less than 40% identity with other known alpha-KTx families. This peptide displayed a neutral amino acid (Leucine), in the position equivalent to lysine 27, described as essential for the interaction with Kv channels. Bot33 did not show any toxicity following i.c.v. injection until 2 \u00b5g\/kg mouse body weight. Due to its very low venom concentration (0.24%), Bot33 was chemically synthesized. Unexpectedly, this peptide has been subjected to a screening on ion channels expressed in Xenopus laevis oocytes, and it was found that Bot33 has no effect on seven Kv channel subtypes. Interestingly, an in silico molecular docking study shows that the Leu27 prevents the interaction of Bot33 with the Kv1.3 channel. All our results indicate that Bot33 may have a different mode of action from other scorpion toxins, which will be interesting to elucidate.<\/jats:p>","DOI":"10.3390\/molecules27217278","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T09:59:37Z","timestamp":1666778377000},"page":"7278","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Purification and Characterization of Bot33: A Non-Toxic Peptide from the Venom of Buthus occitanus tunetanus Scorpion"],"prefix":"10.3390","volume":"27","author":[{"given":"Rym","family":"ElFessi","sequence":"first","affiliation":[{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques, Institut Pasteur de Tunis, Universit\u00e9 de Tunis El Manar, 13 Place Pasteur BP74, Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3617-5705","authenticated-orcid":false,"given":"Oussema","family":"Khamessi","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques, Institut Pasteur de Tunis, Universit\u00e9 de Tunis El Manar, 13 Place Pasteur BP74, Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1296-0909","authenticated-orcid":false,"given":"Najet","family":"Srairi-Abid","sequence":"additional","affiliation":[{"name":"Laboratoire Biomol\u00e9cules, Venins et Applications Th\u00e9ranostiques (LR20IPT01), Institut Pasteur de Tunis, Tunis El Manar, 13 Place Pasteur BP74, Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9040-5647","authenticated-orcid":false,"given":"Jean-Marc","family":"Sabatier","sequence":"additional","affiliation":[{"name":"Institut de Neurophysiopathologie (INP), Universit\u00e9 Aix-Marseille, UMR 7051, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Tytgat","sequence":"additional","affiliation":[{"name":"Toxicology and Pharmacology, Campus Gasthuisberg, University of Leuven (KU Leuven), 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0504-5702","authenticated-orcid":false,"given":"Steve","family":"Peigneur","sequence":"additional","affiliation":[{"name":"Toxicology and Pharmacology, Campus Gasthuisberg, University of Leuven (KU Leuven), 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riadh","family":"Kharrat","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Biomol\u00e9cules Th\u00e9rapeutiques, Institut Pasteur de Tunis, Universit\u00e9 de Tunis El Manar, 13 Place Pasteur BP74, Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/s41573-019-0013-8","article-title":"Antibodies and venom peptides: New therapeutic modalities for ion channels","volume":"18","author":"Wulff","year":"2019","journal-title":"Nat. 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