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Hypertension is the most prevalent risk factor for the development of CVD. Although there is a wide range of antihypertensive drugs, there still remains a lack of blood pressure control options for hypertensive patients. Additionally, natural products remain crucial to the design of new drugs. The natural product 7-hydroxycoumarin (7-HC) exhibits pharmacological properties linked to antihypertensive mechanisms of action. This study aimed to evaluate the vascular effects of 7-HC in an experimental model of essential hypertension. The isometric tension measurements assessed the relaxant effect induced by 7-HC (0.001 \u03bcM\u2013300 \u03bcM) in superior mesenteric arteries isolated from hypertensive rats (SHR, 200\u2013300 g). Our results suggest that the relaxant effect induced by 7-HC rely on K+-channels (KATP, BKCa, and, to a lesser extent, Kv) activation and also on Ca2+ influx from sarcolemma and sarcoplasmic reticulum mobilization (inositol 1,4,5-triphosphate (IP3) and ryanodine receptors). Moreover, 7-HC diminishes the mesenteric artery\u2019s responsiveness to \u03b11-adrenergic agonist challenge and improves the actions of the muscarinic agonist and NO donor. The present work demonstrated that the relaxant mechanism of 7-HC in SHR involves endothelium-independent vasorelaxant factors. Additionally, 7-HC reduced vasoconstriction of the sympathetic agonist while improving vascular endothelium-dependent and independent relaxation.<\/jats:p>","DOI":"10.3390\/molecules27217324","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T09:01:42Z","timestamp":1667120502000},"page":"7324","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["7-Hydroxycoumarin Induces Vasorelaxation in Animals with Essential Hypertension: Focus on Potassium Channels and Intracellular Ca2+ Mobilization"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2674-2024","authenticated-orcid":false,"given":"Rafael L. C.","family":"Jesus","sequence":"first","affiliation":[{"name":"Laboratory of Cardiovascular Physiology and Pharmacology, Federal University of Bahia, Salvador 40110-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isnar L. P.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Physiology and Pharmacology, Federal University of Bahia, Salvador 40110-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6063-1299","authenticated-orcid":false,"given":"F\u00eanix A.","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation\u2014FIOCRUZ, Salvador 40296-710, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9484-8157","authenticated-orcid":false,"given":"Raiana A.","family":"Moraes","sequence":"additional","affiliation":[{"name":"Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation\u2014FIOCRUZ, Salvador 40296-710, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Physiology and Pharmacology, Federal University of Bahia, Salvador 40110-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele S.","family":"Brito","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Physiology and Pharmacology, Federal University of Bahia, Salvador 40110-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela B. C.","family":"Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Physiology and Pharmacology, Federal University of Bahia, Salvador 40110-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quiara L.","family":"Alves","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Physiology and Pharmacology, Federal University of Bahia, Salvador 40110-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darizy F.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Physiology and Pharmacology, Federal University of Bahia, Salvador 40110-902, Brazil"},{"name":"Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation\u2014FIOCRUZ, Salvador 40296-710, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,28]]},"reference":[{"key":"ref_1","unstructured":"GBD 2017 Risk Factor Collaborators (2018). 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