{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:29:52Z","timestamp":1790760592930,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T00:00:00Z","timestamp":1667433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Project of Intergovernmental International Science and Technology Innovation Cooperation","award":["2022YFE0108500"],"award-info":[{"award-number":["2022YFE0108500"]}]},{"name":"Key Project of Intergovernmental International Science and Technology Innovation Cooperation","award":["20ZR1426600"],"award-info":[{"award-number":["20ZR1426600"]}]},{"name":"Natural Science Foundation of Shanghai","award":["2022YFE0108500"],"award-info":[{"award-number":["2022YFE0108500"]}]},{"name":"Natural Science Foundation of Shanghai","award":["20ZR1426600"],"award-info":[{"award-number":["20ZR1426600"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In this study, Scenedesmus sp. FSP3 was cultured using a two-stage culture strategy for CO2 fixation and lutein production. During the first stage, propylene carbonate was added to the medium, with 5% CO2 introduced to promote the rapid growth and CO2 fixation of the microalgae. During the second stage of cultivation, a NaCl concentration of 156 mmol L\u22121 and a light intensity of 160 \u03bcmol m\u22122 s\u22121 were used to stimulate the accumulation of lutein in the microalgal cells. By using this culture method, high lutein production and CO2 fixation were simultaneously achieved. The biomass productivity and carbon fixation rate of Scenedesmus sp. FSP3 reached 0.58 g L\u22121 d\u22121 and 1.09 g L\u22121 d\u22121, with a lutein content and yield as high as 6.45 mg g\u22121 and 2.30 mg L\u22121 d\u22121, respectively. The results reveal a commercially feasible way to integrate microalgal lutein production with CO2 fixation processes.<\/jats:p>","DOI":"10.3390\/molecules27217497","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T03:53:07Z","timestamp":1667447587000},"page":"7497","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["A Two-Stage Culture Strategy for Scenedesmus sp. FSP3 for CO2 Fixation and the Simultaneous Production of Lutein under Light and Salt Stress"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4672-2714","authenticated-orcid":false,"given":"Jiawei","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China"},{"name":"Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, China"},{"name":"Biomass Energy Research Center, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0190-6571","authenticated-orcid":false,"given":"Xinqing","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China"},{"name":"Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo-Shu","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan"},{"name":"Research Center for Smart Sustainable Circular Economy, Tunghai University, Taichung 407, Taiwan"},{"name":"Department of Chemical Engineering, National Cheng-Kung University, Tainan 701, Taiwan"},{"name":"Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 320, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Miao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China"},{"name":"Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, China"},{"name":"Biomass Energy Research Center, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,3]]},"reference":[{"key":"ref_1","unstructured":"International Energy Agency-IEA (2022, August 30). 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