{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:33:09Z","timestamp":1790609589627,"version":"4.1.0"},"reference-count":29,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22077100"],"award-info":[{"award-number":["22077100"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022TD-63"],"award-info":[{"award-number":["2022TD-63"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["17JS007"],"award-info":[{"award-number":["17JS007"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["20200208"],"award-info":[{"award-number":["20200208"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innovation Capability Support Program of Shaanxi","award":["22077100"],"award-info":[{"award-number":["22077100"]}]},{"name":"Innovation Capability Support Program of Shaanxi","award":["2022TD-63"],"award-info":[{"award-number":["2022TD-63"]}]},{"name":"Innovation Capability Support Program of Shaanxi","award":["17JS007"],"award-info":[{"award-number":["17JS007"]}]},{"name":"Innovation Capability Support Program of Shaanxi","award":["20200208"],"award-info":[{"award-number":["20200208"]}]},{"name":"Shaanxi Education Commission","award":["22077100"],"award-info":[{"award-number":["22077100"]}]},{"name":"Shaanxi Education Commission","award":["2022TD-63"],"award-info":[{"award-number":["2022TD-63"]}]},{"name":"Shaanxi Education Commission","award":["17JS007"],"award-info":[{"award-number":["17JS007"]}]},{"name":"Shaanxi Education Commission","award":["20200208"],"award-info":[{"award-number":["20200208"]}]},{"name":"The Youth Talent Promotion Program of Shaanxi University Association for Science and Technology","award":["22077100"],"award-info":[{"award-number":["22077100"]}]},{"name":"The Youth Talent Promotion Program of Shaanxi University Association for Science and Technology","award":["2022TD-63"],"award-info":[{"award-number":["2022TD-63"]}]},{"name":"The Youth Talent Promotion Program of Shaanxi University Association for Science and Technology","award":["17JS007"],"award-info":[{"award-number":["17JS007"]}]},{"name":"The Youth Talent Promotion Program of Shaanxi University Association for Science and Technology","award":["20200208"],"award-info":[{"award-number":["20200208"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Multidrug-resistant bacterial infections mediated by metallo-\u03b2-lactamases (M\u03b2Ls) have grown into an emergent health threat, and development of novel antimicrobials is an ideal strategy to combat the infections. Herein, a novel vancomycin derivative Vb was constructed by conjugation of triazolylthioacetamide and vancomycin molecules, characterized by reverse-phase high performance liquid chromatography (HPLC) and confirmed by matrix-assisted laser desorption\/ionization-time of flight mass spectrometry (MALDI-TOF MS). The biological assays revealed that Vb effectively inhibited S. aureus and methicillin-resistant S. aureus (MRSA), gradually increased the antimicrobial effect of \u03b2-lactam antibiotics (cefazolin, meropenem and penicillin G) and exhibited a dose-dependent synergistic antibacterial effect against eight resistant strains tested, which was confirmed by the time-kill curves determination. Most importantly, Vb increased the antimicrobial effect of meropenem against the clinical isolates EC08 and EC10 and E. coli producing ImiS and CcrA, resulting in a 4- and 8-fold reduction in MIC values, respectively, at a dose up to 32 \u03bcg\/mL. This work offers a promising scaffold for the development of M\u03b2Ls inhibitors, specifically antimicrobials for clinically drug-resistant isolates.<\/jats:p>","DOI":"10.3390\/molecules27227685","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T02:44:32Z","timestamp":1667961872000},"page":"7685","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Modified Vancomycin Molecule Confers Potent Inhibitory Efficacy against Resistant Bacteria Mediated by Metallo-\u03b2-Lactamases"],"prefix":"10.3390","volume":"27","author":[{"given":"Le","family":"Zhai","sequence":"first","affiliation":[{"name":"Shaanxi Key Laboratory of Phytochemistry, Engineering Research Center of Advanced Ferroelectric Functional Materials, College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, College of Life Sciences, Northwest University, Xi\u2019an 710127, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Phytochemistry, Engineering Research Center of Advanced Ferroelectric Functional Materials, College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling-Yan","family":"Kong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, College of Life Sciences, Northwest University, Xi\u2019an 710127, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Xiao","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Phytochemistry, Engineering Research Center of Advanced Ferroelectric Functional Materials, College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Xue","family":"Wang","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Phytochemistry, Engineering Research Center of Advanced Ferroelectric Functional Materials, College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Shi","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Phytochemistry, Engineering Research Center of Advanced Ferroelectric Functional Materials, College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo 726000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2560-9659","authenticated-orcid":false,"given":"Ke-Wu","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, College of Life Sciences, Northwest University, Xi\u2019an 710127, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6750","DOI":"10.1002\/ange.201902210","article-title":"Sulfonium, an underestimated moiety for structural modification, alters antibacterial profile of vancomycin against multidrug-resistant bacteria","volume":"131","author":"Guan","year":"2019","journal-title":"Angew. 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