{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:54:44Z","timestamp":1791230084935,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sichuan Science and Technology Program","award":["2020YFN0147"],"award-info":[{"award-number":["2020YFN0147"]}]},{"name":"Sichuan Science and Technology Program","award":["2021YFH0156"],"award-info":[{"award-number":["2021YFH0156"]}]},{"name":"Sichuan Science and Technology Program","award":["2022YFN0007"],"award-info":[{"award-number":["2022YFN0007"]}]},{"name":"Sichuan Science and Technology Program","award":["2022ZHXC0019"],"award-info":[{"award-number":["2022ZHXC0019"]}]},{"name":"Sichuan Provincial Science and Technology Project for Rural Revitalization","award":["2020YFN0147"],"award-info":[{"award-number":["2020YFN0147"]}]},{"name":"Sichuan Provincial Science and Technology Project for Rural Revitalization","award":["2021YFH0156"],"award-info":[{"award-number":["2021YFH0156"]}]},{"name":"Sichuan Provincial Science and Technology Project for Rural Revitalization","award":["2022YFN0007"],"award-info":[{"award-number":["2022YFN0007"]}]},{"name":"Sichuan Provincial Science and Technology Project for Rural Revitalization","award":["2022ZHXC0019"],"award-info":[{"award-number":["2022ZHXC0019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Salmonella Typhimurium (S. Typhimurium), a common foodborne pathogen, severely harms the public and food security. Type I fimbriae (T1F) of S. Typhimurium, plays a crucial role in the pathogenic processes; it mediates the adhesion of bacteria to the mannose receptor on the host cell, assists the bacteria to invade the host cell, and triggers an inflammatory response. Cinnamaldehyde is the main ingredient in cinnamon essential oil. In this study, cinnamaldehyde was demonstrated to inhibit the expression of T1F by hemagglutination inhibition test, transmission electron microscopy, and biofilms. The mechanism of cinnamaldehyde action was studied by proteomics technology, PCR and Western blotting. The results showed that cinnamaldehyde can inhibit T1F in S. typhimurium without the growth of bacteria, by regulating the level of expression and transcription of fimA, fimZ, fimY, fimH and fimW. Proteomics results showed that cinnamaldehyde downregulated the subunits and regulators of T1F. In addition, the invasion assays proved that cinnamaldehyde can indeed reduce the ability of S. typhimurium to adhere to cells. The results of animal experiments showed that the colonization in the intestinal tract and the expression levels of inflammatory cytokine were significantly decreased, and the intestinal mucosal immune factors MUC1 and MUC2 were increased under cinnamaldehyde treatment. Therefore, cinnamaldehyde may be a potential drug to target T1F to treat Salmonella infections.<\/jats:p>","DOI":"10.3390\/molecules27227753","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T21:33:02Z","timestamp":1668115982000},"page":"7753","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Cinnamaldehyde Resist Salmonella Typhimurium Adhesion by Inhibiting Type I Fimbriae"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8893-9538","authenticated-orcid":false,"given":"Lizi","family":"Yin","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyun","family":"Dai","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuewen","family":"He","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Ouyang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6570-4779","authenticated-orcid":false,"given":"Xiaoli","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangang","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanru","family":"Ai","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Lai","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agriculture University, Huimin Road 211, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S59","DOI":"10.1093\/cid\/ciaa236","article-title":"Global Action for Local Impact: The 11th International Conference on Typhoid and Other Invasive Salmonelloses","volume":"71","author":"Duff","year":"2022","journal-title":"Clin. 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