{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T11:30:20Z","timestamp":1776511820070,"version":"3.51.2"},"reference-count":85,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T00:00:00Z","timestamp":1668643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia","award":["PNURSP2022R65"],"award-info":[{"award-number":["PNURSP2022R65"]}]},{"name":"Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia","award":["GRANT1889"],"award-info":[{"award-number":["GRANT1889"]}]},{"name":"Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, Saudi Arabia","award":["PNURSP2022R65"],"award-info":[{"award-number":["PNURSP2022R65"]}]},{"name":"Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, Saudi Arabia","award":["GRANT1889"],"award-info":[{"award-number":["GRANT1889"]}]},{"name":"Faculty of Medicine, Al-Azhar University, Egypt","award":["PNURSP2022R65"],"award-info":[{"award-number":["PNURSP2022R65"]}]},{"name":"Faculty of Medicine, Al-Azhar University, Egypt","award":["GRANT1889"],"award-info":[{"award-number":["GRANT1889"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In present study, we used Olea europaea leaf extract to biosynthesize in situ Copper Oxide nanocrystals (CuO @OVLe NCs) with powerful antibacterial and anti-cancer capabilities. Physio-chemical analyses, such as UV\/Vis, FTIR, XRD, EDX, SEM, and TEM, were applied to characterize CuO @OVLe NCs. The UV\/Vis spectrum demonstrated a strong peak at 345 nm. Furthermore, FTIR, XRD, and EDX validated the coating operation\u2019s contact with colloidal CuO @OVLe NCs. According to TEM and SEM analyses, CuO @OVLe NCs exhibited a spherical shape and uniform distribution of size with aggregation, for an average size of ~75 nm. The nanoparticles demonstrated a considerable antibacterial effect against E. faecium bacterial growth, as well as an increased inhibition rate in a dose-dependent manner on the MCF-7, PC3, and HpeG2 cancer cell lines and a decreased inhibition rate on WRL-68. Molecular docking and MD simulation were used to demonstrate the high binding affinity of a ligand (Oleuropein) toward the lectin receptor complex of the outer membrane to vancomycin-resistant E. faecium (VREfm) via amino acids (Leu 195, Thr 288, His 165, and Ser 196). Hence, our results expand the accessibility of OVLe\u2019s bioactive components as a promising natural source for the manufacture of physiologically active components and the creation of green biosynthesis of metal nanocrystals.<\/jats:p>","DOI":"10.3390\/molecules27227957","type":"journal-article","created":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T06:11:34Z","timestamp":1668751894000},"page":"7957","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Novel Copper Oxide Bio-Nanocrystals to Target Outer Membrane Lectin of Vancomycin-Resistant Enterococcus faecium (VREfm): In Silico, Bioavailability, Antimicrobial, and Anticancer Potential"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3668-5147","authenticated-orcid":false,"given":"Mahmoud","family":"Kandeel","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Hofuf 31982, Saudi Arabia"},{"name":"Department of Pharmacology, Faculty of Veterinary Medicine, Kafrelshikh University, Kafrelshikh 33516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5891-7378","authenticated-orcid":false,"given":"Mohamed","family":"Sharaf","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Agriculture, AL-Azhar University, Nasr City, Cairo 11651, Egypt"},{"name":"Department of Biochemistry and Molecular Biology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3139-1791","authenticated-orcid":false,"given":"Arshad Mahdi","family":"Hamad","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, Tikrit University, Tikrit 34001, Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6650-9158","authenticated-orcid":false,"given":"Ahmad","family":"O. Babalghith","sequence":"additional","affiliation":[{"name":"Medical Genetics Department, College of Medicine, Umm Al-Qura University, Mecca 24382, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1682-5547","authenticated-orcid":false,"given":"Mohnad","family":"Abdalla","sequence":"additional","affiliation":[{"name":"Pediatric Research Institute, Children\u2019s Hospital Affiliated to Shandong University, Jinan 250022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Arif","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1406-282X","authenticated-orcid":false,"given":"Reem","family":"Binsuwaidan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nashwah","family":"G. M. Attallah","sequence":"additional","affiliation":[{"name":"Egyptian Drug Authority (EDA) Previously NODCAR, Giza 12513, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossam","family":"Aladl Aladl Aladl","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, AL-Azhar University, Nasr City, Cairo 11651, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4025-8586","authenticated-orcid":false,"given":"Samy","family":"Selim","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72388, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariusz","family":"Jaremko","sequence":"additional","affiliation":[{"name":"Smart-Health Initiative and Red Sea Research Center, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13073-016-0294-z","article-title":"The effects of antibiotics on the microbiome throughout development and alternative approaches for therapeutic modulation","volume":"8","author":"Langdon","year":"2016","journal-title":"Genome Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.healthpol.2014.08.013","article-title":"The path of least resistance: Paying for antibiotics in non-human uses","volume":"118","author":"Hollis","year":"2014","journal-title":"Health Policy"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Khalifa, S.A.M., Shedid, E.S., Saied, E.M., Jassbi, A.R., Jamebozorgi, F.H., Rateb, M.E., Du, M., Abdel-Daim, M.M., Kai, G.-Y., and Al-Hammady, M.A.M. 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