{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T03:48:56Z","timestamp":1773373736728,"version":"3.50.1"},"reference-count":102,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T00:00:00Z","timestamp":1668988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007086","name":"Sel\u00e7uk University","doi-asserted-by":"publisher","award":["21212007"],"award-info":[{"award-number":["21212007"]}],"id":[{"id":"10.13039\/501100007086","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In the presented study, the effects of ROCK inhibitor Y-27632, antifreeze protein III, and boron at two different doses were investigated on the spermatological parameters of Ankara buck semen after freeze\u2013thawing. Ejaculates were collected from bucks using an electroejaculator during the breeding season. The ejaculates that showed appropriate characteristics were pooled and used in the dilution and freezing of semen. The extender groups were formed by adding two different doses of three different additives (ROCK inhibitor Y-27632, 5 and 20 \u00b5M; antifreeze protein III, 1 and 4 \u00b5g\/mL; boron, 0.25 and 1 mM) to the control extender. The semen was diluted with the different extenders at 35\u201337 \u00b0C and loaded into straws. Sperm samples frozen in liquid nitrogen vapors, following equilibration, were stored in liquid nitrogen. It was observed that extender supplementation improved post-thaw motility of Ankara buck semen after freeze\u2013thawing. Differences were significant (p &lt; 0.01) for 5 and 10 \u00b5M doses of ROCK inhibitor (71.82% and 74.04 % motility), as well as for 0.25 and 1 mM doses of boron (76.36% and 72.08% motility), compared to the control group (66.15% motility). With respect to the evaluation of acrosomal integrity and mitochondrial activity after freeze\u2013thawing, although supplementation provided protection at all doses, the efficacy was not statistically significant (p &gt; 0.05). It was observed that DNA damage was improved by antifreeze protein III at 1 \u00b5g\/mL (1.23% \u00b1 0.23%) and by boron at all doses (0.25 mM: 1.83% and 1 mM: 1.18%) compared to the control group (3.37%) (p &lt; 0.01), following the thawing process. In the present study, it was determined that some additives added to the extender provided significant improvements in buck spermatozoa motility and DNA damage after thawing.<\/jats:p>","DOI":"10.3390\/molecules27228070","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T04:33:32Z","timestamp":1669005212000},"page":"8070","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Effects of Different Doses of ROCK Inhibitor, Antifreeze Protein III, and Boron Added to Semen Extender on Semen Freezeability of Ankara Bucks"],"prefix":"10.3390","volume":"27","author":[{"given":"\u00d6mer Faruk","family":"Kara\u015f\u00f6r","sequence":"first","affiliation":[{"name":"General Directorate of Agricultural Research and Policies, Ministry of Agriculture and Forestry, Ankara 06800, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa Numan","family":"Bucak","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Sel\u00e7uk University, Konya 42003, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihai","family":"Cenariu","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Faculty, Agricultural Sciences and Veterinary Medicine University, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa","family":"Bodu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Sel\u00e7uk University, Konya 42003, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7598-2665","authenticated-orcid":false,"given":"Mehmet","family":"Ta\u015fp\u0131nar","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Aksaray University, Aksaray 68100, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filiz","family":"Ta\u015fp\u0131nar","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Aksaray University, Aksaray 68100, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,21]]},"reference":[{"key":"ref_1","unstructured":"Yalcin, B.C. (1986). Sheep and Goats in Turkey. Fao Animal Production and Protection Paper, Food and Agriculture Organization of the United Nations."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0921-4488(02)00090-1","article-title":"Studies on improvement of the productivity of Turkish Angora goats by crossing with South African Angora goats","volume":"45","author":"Horst","year":"2002","journal-title":"Small Rumin. Res."},{"key":"ref_3","unstructured":"(2020). Ankara \u0130li Tiftik \u0130\u015fleme Tesisi \u00d6n Fizibilite Raporu, T.C. Sanayi ve Teknoloji Bakanl\u0131\u011f\u0131 Ankara Kalk\u0131nma Ajans\u0131."},{"key":"ref_4","unstructured":"(2022, May 02). Tiftik Raporu, 2020. T.C. Ticaret Bakanl\u0131\u011f\u0131 Esnaf, Sanatk\u00e2rlar ve Kooperatif\u00e7ilik Genel M\u00fcd\u00fcrl\u00fc\u011f\u00fc, Available online: https:\/\/esnafkoop.ticaret.gov.tr\/data\/5d44168e13b876433065544f\/2019%20Tiftik%20Raporu.pdf."},{"key":"ref_5","unstructured":"(2022, February 09). T\u00dc\u0130K, 2022. \u0130statistiksel Tablolar, K\u00fc\u00e7\u00fckba\u015f Hayvan Say\u0131lar\u0131, Available online: https:\/\/data.tuik.gov.tr\/Kategori\/GetKategori?p=tarim-111&dil=1."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/164666a0","article-title":"Revival of spermatozoa after vitrification and dehydration at low temperatures","volume":"164","author":"Polge","year":"1949","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1071\/RD9950871","article-title":"Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function","volume":"7","author":"Watson","year":"1995","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.smallrumres.2005.02.015","article-title":"A review on goat sperm cryopreservation","volume":"63","author":"Purdy","year":"2006","journal-title":"Small Rumin. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Quain, M. (2019). Cryobiology and cryopreservation of sperm. Cryopreservation-Current Advances and Evaluations, IntechOpen.","DOI":"10.5772\/intechopen.77775"},{"key":"ref_10","first-page":"59","article-title":"Effect of some cryoprotectans in cryopreservation of Angora Buck semen","volume":"48","author":"Bucak","year":"2008","journal-title":"J. Lalahan Livest. Res. Inst."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.smallrumres.2008.11.011","article-title":"Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation","volume":"81","author":"Bucak","year":"2009","journal-title":"Small Rumin. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.smallrumres.2009.11.015","article-title":"The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities","volume":"89","author":"Bucak","year":"2010","journal-title":"Small Rumin. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.theriogenology.2009.09.015","article-title":"Effects of different extenders and centrifugation\/washing on postthaw microscopic-oxidative stress parameters and fertilizing ability of Angora buck sperm","volume":"73","author":"Bucak","year":"2010","journal-title":"Theriogenology"},{"key":"ref_14","unstructured":"Pugh, D.G., Baird, N.N., Edmondson, M., and Passler, T. (2020). Theriogenology of sheep, goats, and cervids. Sheep, Goat, and Cervid Medicine, Elsevier Health Sciences."},{"key":"ref_15","unstructured":"Bazer, F.W., Lamb, G.C., and Wu, G. (2020). Reproductive management of sheep and goats. Animal Agriculture, Elsevier."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1530\/jrf.0.0910451","article-title":"Cryopreservation, actin localization and thermotropic phase transitions in ram spermatozoa","volume":"91","author":"Holt","year":"1991","journal-title":"Reproduction"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.cryobiol.2020.09.001","article-title":"Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses","volume":"96","author":"Bucak","year":"2020","journal-title":"Cryobiology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1089\/bio.2020.0056","article-title":"Cryopreservation effects on ram sperm ultrastructure","volume":"18","author":"Keskin","year":"2020","journal-title":"Biopreserv. Biobank."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.cryobiol.2020.03.008","article-title":"The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters","volume":"95","author":"Bodu","year":"2020","journal-title":"Cryobiology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1111\/andr.12974","article-title":"Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze-thawed ram spermatozoa","volume":"9","author":"Bucak","year":"2021","journal-title":"Andrology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/andr.13130","article-title":"Combination of trehalose and low boron in presence of decreased glycerol improves post-thawed ram sperm parameters: A model study in boron research","volume":"10","author":"Bucak","year":"2022","journal-title":"Andrology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0378-4320(00)00152-4","article-title":"Basic aspects of frozen storage of semen","volume":"62","author":"Holt","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_23","first-page":"67","article-title":"Kryoprotektanlar ve gamet h\u00fccrelerinin dondurulmas\u0131nda kryoprotektif etki","volume":"54","author":"Bucak","year":"2007","journal-title":"Ank. \u00dcniv. Vet. Fak. Derg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/0011-2240(92)90009-Q","article-title":"Predictive water loss curves for ram spermatozoa during cryopreservation: Comparison with experimental observations","volume":"29","author":"Duncan","year":"1992","journal-title":"Cryobiology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0093-691X(01)00674-4","article-title":"Current status of sperm cryopreservation: Why isn\u2019t it better?","volume":"57","author":"Medeiros","year":"2002","journal-title":"Theriogenology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1083\/jcb.57.3.729","article-title":"Visualization of freezing damage","volume":"57","author":"Bank","year":"1973","journal-title":"J. Cell Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1088\/0965-0393\/15\/2\/004","article-title":"A texture-component Avrami model for predicting recrystallization textures, kinetics and grain size","volume":"15","author":"Raabe","year":"2006","journal-title":"Model Simul. Mater. Sci. Eng."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/0011-2240(77)90176-6","article-title":"Use of two-step cooling procedures to examine factors influencing cell survival following freezing and thawing","volume":"14","author":"Farrant","year":"1977","journal-title":"Cryobiology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/0011-2240(83)90043-3","article-title":"Effects of various degrees of supercooling and nucleation temperatures on fertility of frozen turkey spermatozoa","volume":"20","author":"Zavos","year":"1983","journal-title":"Cryobiology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.fertnstert.2008.09.038","article-title":"Effects of cryopreservation on human sperm deoxyribonucleic acid integrity","volume":"93","author":"Zribi","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1095\/biolreprod.103.024281","article-title":"Cryopreservation induces an apoptosis-like mechanism in bull sperm","volume":"71","author":"Martin","year":"2004","journal-title":"Biol. Reprod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1016\/j.fertnstert.2008.02.173","article-title":"Evaluation of poly (ADP-ribose) polymerase cleavage (cPARP) in ejaculated human sperm fractions after induction of apoptosis","volume":"91","author":"Mahfouz","year":"2009","journal-title":"Fertil. Steril."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1002\/(SICI)1097-010X(19991101)284:6<696::AID-JEZ11>3.0.CO;2-E","article-title":"Fertilizing ability of DNA damaged spermatozoa","volume":"284","author":"Ahmadi","year":"1999","journal-title":"J. Exp. Zool."},{"key":"ref_34","unstructured":"Rodriguez-Martinez, H., Vallet, J.L., and Ziecik, A.J. (2009). Recent advances in boar semen cryopreservation. Control of Pig Reproduction VIII, Nottingham University Press."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.rvsc.2009.04.014","article-title":"Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stress parameters of Angora goat semen","volume":"87","author":"Bucak","year":"2009","journal-title":"Res. Vet. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.cryobiol.2010.05.005","article-title":"The effect of raffinose and methionine on frozen\/thawed Angora buck (Capra hircus ancryrensis) semen quality, lipid peroxidation and antioxidant enzyme activities","volume":"61","author":"Tuncer","year":"2010","journal-title":"Cryobiology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1126\/science.163.3871.1073","article-title":"Freezing resistance in some Antarctic Fishes","volume":"163","author":"DeVries","year":"1969","journal-title":"Science"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1021\/ja047652y","article-title":"Ice surface reconstruction as antifreeze protein-induced morphological modification mechanism","volume":"127","author":"Strom","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"ref_39","unstructured":"Sun, D.W. (2005). Controlling the freezing process with antifreeze proteins. Emerging Technologies for Food Processing, Elsevier Academic Press."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.cryobiol.2006.06.006","article-title":"The basis for hyperactivity of antifreeze proteins","volume":"53","author":"Scotter","year":"2006","journal-title":"Cryobiology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.theriogenology.2007.05.003","article-title":"The antifreeze protein type I (AFP I) increases seabream (Sparus aurata) embryos tolerance to low temperatures","volume":"68","author":"Robles","year":"2007","journal-title":"Theriogenology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1016\/j.lwt.2007.07.010","article-title":"Effect of carrot (Daucus carota) antifreeze proteins on texture properties of frozen dough and volatile compounds of crumb","volume":"41","author":"Zhang","year":"2008","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.jfoodeng.2009.05.013","article-title":"The response of watercress (Nasturtium officinale) to vacuum impregnation: Effect of an antifreeze protein type I","volume":"95","author":"Cruz","year":"2009","journal-title":"J. Food Eng."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.cryobiol.2009.01.007","article-title":"Cold-regulated proteins with potent antifreeze and cryoprotective activities in spruces (Picea spp.)","volume":"58","author":"Pukacki","year":"2009","journal-title":"Cryobiology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1016\/S0006-291X(05)80939-8","article-title":"The effect of antifreeze glycopeptides on membrane potential changes at hypothermic temperatures","volume":"173","author":"Rubinsky","year":"1990","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s00792-012-0494-4","article-title":"Characterization of Afp1, an antifreeze protein from the psychrophilic yeast Glaciozyma antarctica PI12","volume":"17","author":"Hashim","year":"2013","journal-title":"Extremophiles"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1002\/mrd.1080360413","article-title":"Cryogenic protection of oocytes with antifreeze proteins","volume":"36","author":"Arav","year":"1993","journal-title":"Mol. Reprod. Dev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1006\/cryo.1994.1021","article-title":"Effect of antifreeze proteins on the motility of ram spermatozoa","volume":"31","author":"Payne","year":"1994","journal-title":"Cryobiology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1002\/(SICI)1096-9098(199710)66:2<114::AID-JSO8>3.0.CO;2-G","article-title":"Chemical adjuvant cryosurgery with antifreeze proteins","volume":"66","author":"Koushafar","year":"1997","journal-title":"J. Surg. Oncol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1046\/j.1432-1033.2003.03488.x","article-title":"\u2018Antifreeze\u2019glycoproteins from polar fish","volume":"270","author":"Harding","year":"2003","journal-title":"Eur. J. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.anireprosci.2015.03.015","article-title":"Efficiency of antifreeze glycoproteins for cryopreservation of Nili-Ravi (Bubalus bubalis) buffalo bull sperm","volume":"157","author":"Qadeer","year":"2015","journal-title":"Anim. Reprod. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1038\/nrm1128","article-title":"Rocks: Multifunctional kinases in cell behaviour","volume":"4","author":"Riento","year":"2003","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0092-8674(04)00003-0","article-title":"Rho and Rac take center stage","volume":"116","author":"Burridge","year":"2004","journal-title":"Cell"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1083\/jcb.200510062","article-title":"An inhibitory role for FAK in regulating proliferation: A link between limited adhesion and RhoA-ROCK signaling","volume":"174","author":"Pirone","year":"2006","journal-title":"J. Cell Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1017\/S0967199406003790","article-title":"Localization of the Rho GTPases and some Rho effector proteins in the sperm of several mammalian species","volume":"14","author":"Ducummon","year":"2006","journal-title":"Zygote"},{"key":"ref_56","first-page":"3645","article-title":"Rho kinase proteins\u2014Pleiotropic modulators of cell survival and apoptosis","volume":"31","author":"Street","year":"2011","journal-title":"Anticancer Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.ajpath.2011.10.036","article-title":"ROCK inhibitor and feeder cells induce the conditional reprogramming of epithelial cells","volume":"180","author":"Liu","year":"2012","journal-title":"Am. J. Pathol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/0014-5793(96)00811-3","article-title":"ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine\/threonine kinase in mice","volume":"392","author":"Nakagawa","year":"1996","journal-title":"FEBS Lett."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.tips.2007.04.006","article-title":"Development of Rho-kinase inhibitors for cardiovascular medicine","volume":"28","author":"Shimokawa","year":"2007","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"49911","DOI":"10.1074\/jbc.M306079200","article-title":"Engulfment of apoptotic cells is negatively regulated by Rho-mediated signaling","volume":"278","author":"Ravichandran","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1007\/s10495-007-0173-6","article-title":"Rho-kinase-dependent F-actin rearrangement is involved in the inhibition of PI3-kinase\/Akt during ischemia\u2013reperfusion-induced endothelial cell apoptosis","volume":"13","author":"Versteilen","year":"2008","journal-title":"Apoptosis"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1165\/rcmb.2003-0019OC","article-title":"Rho kinase inhibition initiates apoptosis in human airway epithelial cells","volume":"30","author":"Moore","year":"2004","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_63","unstructured":"(2022, April 01). Eti Maden \u0130\u015fletmeleri Genel M\u00fcd\u00fcrl\u00fc\u011f\u00fc, 2022. D\u00fcnya\u2019da Bor, Available online: https:\/\/www.etimaden.gov.tr\/dunyada-bor."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1017\/S000711450770911X","article-title":"Estimation of human daily boron exposure in a boron-rich area","volume":"98","author":"Korkmaz","year":"2007","journal-title":"Br. J. Nutr."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1007\/s12011-010-8928-2","article-title":"Effects of chronic boron exposure on semen profile","volume":"143","author":"Korkmaz","year":"2011","journal-title":"Biol. Trace. Elem. Res."},{"key":"ref_66","first-page":"211","article-title":"Dietary boron affects blood cell counts and hemoglobin concentrations in humans","volume":"4","author":"Nielsen","year":"1991","journal-title":"J. Trace. Elem. Exp. Med."},{"key":"ref_67","first-page":"31","article-title":"Proposed physiologic functions of boron in plants pertinent to animal and human metabolism","volume":"102","author":"Blevins","year":"1994","journal-title":"Environ. Health Perspect."},{"key":"ref_68","first-page":"35","article-title":"The biochemical effects of physiologic amounts of dietary boron in animal nutrition models","volume":"102","author":"Hunt","year":"1994","journal-title":"Environ. Health Perspect."},{"key":"ref_69","unstructured":"WHO (1996). Trace Elements in Human Nutrition and Health, World Health Organization."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3923\/ajpsaj.2015.85.96","article-title":"Dietary boron supplementation and its impact on semen characteristics and physiological status of adult male rabbits","volume":"9","author":"Elkomy","year":"2015","journal-title":"Asian J. Poult. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0041-008X(72)90037-3","article-title":"Toxicologic studies on borax and boric acid","volume":"23","author":"Fisher","year":"1972","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1006\/faat.1996.0121","article-title":"Developmental toxicity NOAEL and postnatal recovery in rats fed boric acid during gestation","volume":"32","author":"Price","year":"1996","journal-title":"Fundam. Appl. Toxicol."},{"key":"ref_73","first-page":"87","article-title":"The reproductive toxicity of boric acid","volume":"102","author":"Chapin","year":"1994","journal-title":"Environ. Health Perspect."},{"key":"ref_74","unstructured":"Evans, G., Maxwell, W.M.C., and Salamon, S. (1987). Salamon\u2019s Artificial Insemination of Sheep and Goats, Butterworths."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1095\/biolreprod53.2.276","article-title":"Viability assessment of mammalian sperm using SYBR-14 and propidium iodide","volume":"53","author":"Garner","year":"1995","journal-title":"Biol. Reprod."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1095\/biolreprod.102.011445","article-title":"A triple-stain flow cytometric method to assess plasma-and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles","volume":"68","author":"Nagy","year":"2003","journal-title":"Biol. Reprod."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1095\/biolreprod57.6.1401","article-title":"Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa","volume":"57","author":"Garner","year":"1997","journal-title":"Biol. Reprod."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1111\/andr.12324","article-title":"An investigation of the potential effect of sperm nuclear vacuoles in human spermatozoa on DNA fragmentation using a neutral and alkaline Comet assay","volume":"5","author":"Pastuszek","year":"2017","journal-title":"Andrology"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1186\/s40104-022-00754-8","article-title":"Determination of double- and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity","volume":"13","author":"Hidalgo","year":"2022","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1126\/science.175.4023.720","article-title":"The Fluid Mosaic Model of the Structure of Cell Membranes: Cell membranes are viewed as two-dimensional solutions of oriented globular proteins and lipids","volume":"175","author":"Singer","year":"1972","journal-title":"Science"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1194\/jlr.R120000800","article-title":"Roles of polyunsaturated fatty acids, from mediators to membranes","volume":"61","author":"Harayama","year":"2020","journal-title":"J. Lipid Res."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.marenvres.2018.03.007","article-title":"Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice","volume":"137","author":"Bharudin","year":"2018","journal-title":"Mar. Environ. Res."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/j.1939-4640.1990.tb01583.x","article-title":"Cryopreservation of mammalian sperm: What we ask them to survive","volume":"11","author":"Hammerstedt","year":"1990","journal-title":"J. Androl."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.theriogenology.2019.12.015","article-title":"Optimal concentration of Rho-associated coiled-coil kinase (ROCK) inhibitor improved sperm membrane functionality and fertilizing ability of cryopreserved-thawed feline sperm","volume":"144","author":"Tharasanit","year":"2020","journal-title":"Theriogenology"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.theriogenology.2014.02.020","article-title":"ROCK inhibitor Y-27632 prevents porcine oocyte maturation","volume":"82","author":"Zhang","year":"2014","journal-title":"Theriogenology"},{"key":"ref_86","first-page":"322","article-title":"Rho\/ROCK signaling pathway and anti-cryodamage ability of human sperm","volume":"25","author":"Tian","year":"2019","journal-title":"Natl. J. Androl."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/S0278-6915(02)00042-X","article-title":"Antifreeze proteins: Characteristics, occurrence and human exposure","volume":"40","author":"Crevel","year":"2002","journal-title":"Food Chem. Toxicol."},{"key":"ref_88","first-page":"27","article-title":"Antifiriz proteinler","volume":"18","year":"2007","journal-title":"Etlik. Vet. Mikrobiyol. Derg."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"3189","DOI":"10.1111\/jfpp.12476","article-title":"Antifreeze proteins: Characteristics, function, mechanism of action, sources and application to foods","volume":"39","author":"Ustun","year":"2015","journal-title":"J. Food Process. Preserv."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/S0006-291X(05)81102-7","article-title":"Hypothermic protection-a fundamental property of \u201cantifreeze\u201d proteins","volume":"180","author":"Rubinsky","year":"1991","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.cryobiol.2020.11.001","article-title":"Addition of antifreeze protein type I or III to extenders for ram sperm cryopreservation","volume":"98","author":"Correia","year":"2021","journal-title":"Cryobiology"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1089\/bio.2020.0140","article-title":"The Effects of Antifreeze Protein III Supplementation on the Cryosurvival of Goat Spermatozoa During Cryopreservation","volume":"19","author":"Lv","year":"2021","journal-title":"Biopreserv. Biobank."},{"key":"ref_93","first-page":"331","article-title":"Different regulatory systems operate in the midpiece and principal piece of the mammalian sperm flagellum","volume":"65","author":"Suarez","year":"2007","journal-title":"Soc. Reprod. Fertil. Suppl."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3233\/JAD-2007-12210","article-title":"Lipoic acid and N-acetyl cysteine decrease mitochondrial-related oxidative stress in Alzheimer disease patient fibroblasts","volume":"12","author":"Moreira","year":"2007","journal-title":"J. Alzheimer\u2019s Dis."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1038\/sj.ki.5002568","article-title":"The role of mitochondria in oxidative and nitrosative stress during ischemia\/reperfusion in the rat kidney","volume":"72","author":"Plotnikov","year":"2007","journal-title":"Kidney Int."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.cryobiol.2014.04.014","article-title":"Effects of type III antifreeze protein on sperm and embryo cryopreservation in rabbit","volume":"69","author":"Nishijima","year":"2014","journal-title":"Cryobiology"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.jtemb.2019.05.004","article-title":"Dietary boron supplementation enhances sperm quality and immunity through influencing the associated biochemical parameters and modulating the genes expression at testicular tissue","volume":"55","author":"Krishnan","year":"2019","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_98","first-page":"53","article-title":"The effect of Boron addition on spermatological parameters, oxidative stress and DNA damage after frozen-thawed process in ramlic ram semen","volume":"32","author":"Yeni","year":"2018","journal-title":"F\u0131rat. \u00dcniv. Sa\u011fl\u0131k. Bilim. Vet. Derg."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1093\/humrep\/13.6.1429","article-title":"Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: Lipid peroxidation, DNA fragmentation and effectiveness of antioxidants","volume":"13","author":"Twigg","year":"1998","journal-title":"Hum. Reprod."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S1383-5718(01)00300-X","article-title":"Oxidative stress associated DNA damage in testis of mice: Induction of abnormal sperms and effects on fertility","volume":"513","author":"Kumar","year":"2002","journal-title":"Mutat. Res.\/Genet. Toxicol. Environ. Mutagen."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1002\/j.1939-4640.1992.tb03327.x","article-title":"Reactive oxygen species and human spermatozoa: I. Effects on the motility of intact spermatozoa and on sperm axonemes","volume":"13","author":"Gagnon","year":"1992","journal-title":"J. Androl."},{"key":"ref_102","unstructured":"Hafez, E.S.E. (1993). Spermatozoa and seminal plasma. Reproduction in Farm Animals, Lea&Febiger."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/22\/8070\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:22:30Z","timestamp":1760145750000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/22\/8070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,21]]},"references-count":102,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["molecules27228070"],"URL":"https:\/\/doi.org\/10.3390\/molecules27228070","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,21]]}}}