{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T09:14:52Z","timestamp":1781860492245,"version":"3.54.5"},"reference-count":33,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T00:00:00Z","timestamp":1669075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AECID","award":["IPT-060000-2010-14"],"award-info":[{"award-number":["IPT-060000-2010-14"]}]},{"name":"AECID","award":["CTQ2012-37573-C02-2015-69278-C2"],"award-info":[{"award-number":["CTQ2012-37573-C02-2015-69278-C2"]}]},{"name":"MINECO","award":["IPT-060000-2010-14"],"award-info":[{"award-number":["IPT-060000-2010-14"]}]},{"name":"MINECO","award":["CTQ2012-37573-C02-2015-69278-C2"],"award-info":[{"award-number":["CTQ2012-37573-C02-2015-69278-C2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Fluoroquinolones (FQs) are broad-spectrum antibiotics widely used to treat animal and human infections. The use of FQs in these activities has increased the presence of antibiotics in wastewater and food, triggering antimicrobial resistance, which has severe consequences for human health. The detection of antibiotics residues in water and food samples has attracted much attention. Herein, we report the development of a highly sensitive online solid-phase extraction methodology based on a selective molecularly imprinted polymer (MIP) and fluorescent detection (HPLC-FLD) for the determination of FQs in water at low ng L\u22121 level concentration. Under the optimal conditions, good linearity was obtained ranging from 0.7 to 666 ng L\u22121 for 7 FQs, achieving limits of detection (LOD) in the low ng L\u22121 level and excellent precision. Recoveries ranged between 54 and 118% (RSD &lt; 17%) for all the FQs tested. The method was applied to determining FQs in river water. These results demonstrated that the developed method is highly sensitive and selective.<\/jats:p>","DOI":"10.3390\/molecules27238120","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T08:29:58Z","timestamp":1669105798000},"page":"8120","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The Ultratrace Determination of Fluoroquinolones in River Water Samples by an Online Solid-Phase Extraction Method Using a Molecularly Imprinted Polymer as a Selective Sorbent"],"prefix":"10.3390","volume":"27","author":[{"given":"A. N.","family":"Baeza","sequence":"first","affiliation":[{"name":"Institute of Science and Technology of Materials, University of Havana, Zapata y G, La Habana 10400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idoia","family":"Urriza-Arsuaga","sequence":"additional","affiliation":[{"name":"Independent Researcher, 28007 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Navarro-Villoslada","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias, 2, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier L.","family":"Urraca","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias, 2, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,22]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2022, October 10). Antibiotic Resistance. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/antibiotic-resistance."},{"key":"ref_2","first-page":"697","article-title":"Resistance to antibiotics: Are we in the post-antibiotic era?","volume":"36","author":"Alanis","year":"2005","journal-title":"Med. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1021\/es032519b","article-title":"Are veterinary medicines causing environmental risks?","volume":"37","author":"Boxall","year":"2003","journal-title":"Environ. Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3632","DOI":"10.1021\/ac0015265","article-title":"Trace determination of fluoroquinolone antibacterial agents in urban wastewater by solid-phase extraction and liquid chromatography with fluorescence detection","volume":"73","author":"Golet","year":"2001","journal-title":"Anal. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Wang, J., MacNeil, J.D., and Kay, J.F. (2012). Chemical Analysis of Antibiotic Residues in Food, Wiley.","DOI":"10.1002\/9781118067208"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1136\/bmj.317.7159.647","article-title":"Antimicrobial resistance in developing countries","volume":"317","author":"Hart","year":"1998","journal-title":"Br. Med. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.1021\/es048143z","article-title":"Screening of human antibiotic substances and determination of weekly mass flows in five sewage treatment plants in Sweden","volume":"39","author":"Lindberg","year":"2005","journal-title":"Environ. Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.aca.2009.07.031","article-title":"An overview of analytical methodologies for the determination of antibiotics in environmental waters","volume":"649","author":"Lino","year":"2009","journal-title":"Anal. Chim. Acta"},{"key":"ref_9","first-page":"250","article-title":"Fluoroquinolone antibiotics: An emerging class of environmental micropollutants","volume":"500\u2013501","author":"Dewulf","year":"2014","journal-title":"Sci. Total Environ."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3645","DOI":"10.1021\/es0256212","article-title":"Environmental exposure and risk assessment of fluoroquinolone antibacterial agents in wastewater and river water of the Glatt Valley Watershed, Switzerland","volume":"36","author":"Golet","year":"2002","journal-title":"Environ. Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.aca.2006.01.053","article-title":"Determination of fluoroquinolones in environmental waters by in-tube solid-phase microextraction coupled with liquid chromatography\u2013tandem mass spectrometry","volume":"562","author":"Mitani","year":"2006","journal-title":"Anal. Chim. Acta"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1021\/es102473z","article-title":"Antibiotic resistance gene abundances associated with waste discharges to the Almendares River near Havana, Cuba","volume":"45","author":"Graham","year":"2011","journal-title":"Environ. Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.microc.2013.06.001","article-title":"Chemiluminescence analysis of enrofloxacin in surface water using the tris(1,10-phenantroline)\u2013ruthenium(II)\/peroxydisulphate system and extraction with molecularly imprinted polymers","volume":"110","author":"Luaces","year":"2013","journal-title":"Microchem. J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1002\/jssc.201400223","article-title":"Multiresidue analysis of quinolones in water by Ultra high performance liquid chromatography tandem mass spectrometry using a simple and effective sample treatment","volume":"37","year":"2014","journal-title":"J. Sep. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/j.scitotenv.2014.06.082","article-title":"Development of sensitive and reliable LC-MS\/MS methods for the determination of three fluoroquinolones in water and fish tissue samples and preliminary environmental risk assessment of their presence in two rivers in northern Poland","volume":"493","author":"Wagil","year":"2014","journal-title":"Sci. Total Environ."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1080\/00032710902890363","article-title":"Quick and sensitive determination of fluoroquinolones by capillary electrophoresis\u2013potential gradient detection","volume":"42","author":"Fan","year":"2009","journal-title":"Anal. Lett."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1002\/jssc.201401216","article-title":"Magnetic mixed hemimicelles solid-phase extraction coupled with high-performance liquid chromatography for the extraction and rapid determination of six fluoroquinolones in environmental water samples","volume":"38","author":"Wang","year":"2015","journal-title":"J. Sep. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.chroma.2015.09.066","article-title":"Simultaneous determination of fluoroquinolones in environmental water by liquid chromatography\u2013tandem mass spectrometry with direct injection: A green approach","volume":"1418","author":"Denadai","year":"2015","journal-title":"J. Chromatogr. A"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1002\/jssc.201600232","article-title":"Determination of fluoroquinolones in environmental water and milk samples treated with stir cake sorptive extraction based on a boron-rich monolith","volume":"39","author":"Mei","year":"2016","journal-title":"J. Sep. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1007\/s00604-016-1859-7","article-title":"Preparation of silica-supported carbon by Kraft lignin pyrolysis, and its use in solid-phase extraction of fluoroquinolones from environmental waters","volume":"183","author":"Speltini","year":"2016","journal-title":"Microchim. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1021\/ac102839n","article-title":"Multiresidue determination of ultratrace levels of fluoroquinolone antimicrobials in drinking and aquaculture water samples by automated online molecularly imprinted solid phase extraction and liquid chromatography","volume":"83","year":"2011","journal-title":"Anal. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.aca.2010.01.001","article-title":"Determination of fluoroquinolone antibiotics in environmental water samples based on magnetic molecularly imprinted polymer extraction followed by liquid chromatography\u2013tandem mass spectrometry","volume":"662","author":"Chen","year":"2010","journal-title":"Anal. Chim. Acta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.jhazmat.2012.11.003","article-title":"Preparation of molecularly imprinted polymer nanoparticles for selective removal of fluoroquinolone antibiotics in aqueous solution","volume":"244\u2013245","author":"Tan","year":"2013","journal-title":"J. Hazard. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"17293","DOI":"10.1039\/C8RA01837E","article-title":"Fast quantification of fluoroquinolones in environmental water samples using molecularly imprinted polymers coupled with internal extractive electrospray ionization mass spectrometry","volume":"8","author":"Kou","year":"2018","journal-title":"RSC Adv."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chroma.2014.03.045","article-title":"Multiresidue analysis of fluoroquinolone antimicrobials in chicken meat by molecularly imprinted solid-phase extraction and high performance liquid chromatography","volume":"1343","author":"Urraca","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"113927","DOI":"10.1016\/j.envpol.2020.113927","article-title":"Quantification of fluoroquinolones in wastewaters by liquid chromatography-tandem mass spectrometry","volume":"259","author":"Maia","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.aca.2012.08.042","article-title":"Determination of eight fluoroquinolones in groundwater samples with ultrasound-assisted ionic liquid dispersive liquid\u2013liquid microextraction prior to high-performance liquid chromatography and fluorescence detection","volume":"748","year":"2012","journal-title":"Anal. Chim. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.aca.2020.05.071","article-title":"Room-temperature synthesis of flower-shaped covalent organic frameworks for solid-phase extraction of quinolone antibiotics","volume":"1126","author":"Xu","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"462286","DOI":"10.1016\/j.chroma.2021.462286","article-title":"Determination of quinolones in environmental water and fish by magnetic metal organic frameworks based magnetic solid-phase extraction followed by high-performance liquid chromatography-tandem mass spectrometry","volume":"1651","author":"Wang","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"105955","DOI":"10.1016\/j.microc.2021.105955","article-title":"Cyclodextrin-derivatized hybrid nanocomposites as novel magnetic solid-phase extraction adsorbent for preconcentration of trace fluoroquinolones from water samples coupled with HPLC-MS\/MS determination","volume":"164","author":"Li","year":"2021","journal-title":"Microchem. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1007\/s00604-019-4033-1","article-title":"A nanofiber mat prepared from sulfonated polyaniline for solid-phase extraction of fluoroquinolones from water and biological fluids prior to their quantitation by UPLC-MS\/MS","volume":"186","author":"Jian","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5357","DOI":"10.1021\/acsami.0c17310","article-title":"Ionic liquid-functionalized magnetic metal\u2212organic framework nanocomposites for efficient extraction and sensitive detection of fluoroquinolone antibiotics in environmental water","volume":"13","author":"Lu","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"129632","DOI":"10.1016\/j.snb.2021.129632","article-title":"A novel electrochemical approach for the ultrasensitive detection of fluoroquinolones based on a double-labelled aptamer to surpass complementary strands of aptamer lying flat","volume":"334","author":"Heidarian","year":"2021","journal-title":"Sens. Actuators B Chem."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/23\/8120\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:24:14Z","timestamp":1760145854000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/23\/8120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,22]]},"references-count":33,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["molecules27238120"],"URL":"https:\/\/doi.org\/10.3390\/molecules27238120","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,22]]}}}