{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:28:33Z","timestamp":1793312913233,"version":"4.5.0"},"reference-count":19,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,26]],"date-time":"2022-11-26T00:00:00Z","timestamp":1669420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973825"],"award-info":[{"award-number":["81973825"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20366"],"award-info":[{"award-number":["U22A20366"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["GXXT-2020-025"],"award-info":[{"award-number":["GXXT-2020-025"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Regional Innovation and Development Joint Fund","award":["81973825"],"award-info":[{"award-number":["81973825"]}]},{"name":"Regional Innovation and Development Joint Fund","award":["U22A20366"],"award-info":[{"award-number":["U22A20366"]}]},{"name":"Regional Innovation and Development Joint Fund","award":["GXXT-2020-025"],"award-info":[{"award-number":["GXXT-2020-025"]}]},{"name":"Collaborative Innovation Project of Anhui Colleges and Universities","award":["81973825"],"award-info":[{"award-number":["81973825"]}]},{"name":"Collaborative Innovation Project of Anhui Colleges and Universities","award":["U22A20366"],"award-info":[{"award-number":["U22A20366"]}]},{"name":"Collaborative Innovation Project of Anhui Colleges and Universities","award":["GXXT-2020-025"],"award-info":[{"award-number":["GXXT-2020-025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Gandouling (GDL) Pill is a novel Traditional Chinese medicinal drug to treat Wilson\u2019s disease in clinics. It is composed of six separate herbal medicines, including Rhei Radix ET Rhizoma, Coptidis Rhizoma, Salviae Miltiorrhizae Radix ET Rhizoma, Spatholobi Caulis, Curcumae Rhizoma, and Curcumae Longae Rhizoma. In this study, a strategy was proposed to investigate the chemical constituents and to quantify the potential bioactive components in GDL Pill. Firstly, the mass fragmentation behaviors of representative compounds were investigated, and, in total, 69 compounds were characterized in GDL Pill using full scan\/dd-MS2 scan mode by ultra-high-performance liquid chromatography (UPLC)\/Q-Orbitrap mass spectrometry (MS). These compounds included 18 alkaloids, 18 ketones, 16 phenolic compounds, 11 organic acids, and 6 tanshinones. Seventeen of the compounds were unambiguously identified by comparison with reference standards. Secondly, the absorption components of GDL Pill in rat plasma were investigated by using target-Selected Ion Monitoring (t-SIM) scan mode built in Q-Orbitrap MS. A total of 18 components were detected, which were considered as potential bioactive components of GDL Pill. Thirdly, 10 major absorption components were simultaneously determined in six batches of samples by UPLC\/diode array detector (DAD). The method was fully validated with respect to linearity, precision, repeatability, stability, and recovery. Alkaloids from Coptidis Rhizoma, such as coptisine (8), berberine (18), palmatine (19), were the most abundant bioactive compounds for GDL Pill that possess the potential be used as quality markers. The proposed strategy is practical and efficient for revealing the material basis of GDL Pill, and also provides a simple and accurate method for quality control.<\/jats:p>","DOI":"10.3390\/molecules27238247","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T07:38:49Z","timestamp":1669621129000},"page":"8247","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chemical Profiling and Quantification of Potential Bioactive Components in Gandouling Pill by Ultra-High Performance Liquid Chromatography Coupled with Diode Array Detector\/Quadruple-Qrbitrap Mass Spectrometry"],"prefix":"10.3390","volume":"27","author":[{"given":"Yue","family":"Yang","sequence":"first","affiliation":[{"name":"Graduate School of Anhui University of Chinese Medicine, Hefei 230038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Hao","sequence":"additional","affiliation":[{"name":"Graduate School of Anhui University of Chinese Medicine, Hefei 230038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulong","family":"Yang","sequence":"additional","affiliation":[{"name":"Graduate School of Anhui University of Chinese Medicine, Hefei 230038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affifiliated Hospital of Anhui University of Chinese Medicine, Hefei 230022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affifiliated Hospital of Anhui University of Chinese Medicine, Hefei 230022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meixia","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affifiliated Hospital of Anhui University of Chinese Medicine, Hefei 230022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Dong","sequence":"additional","affiliation":[{"name":"The First Affifiliated Hospital of Anhui University of Chinese Medicine, Hefei 230022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1576-7867","authenticated-orcid":false,"given":"Zhanpeng","family":"Shang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenming","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Affifiliated Hospital of Anhui University of Chinese Medicine, Hefei 230022, China"},{"name":"Xin\u2019an Medical Education Ministry Key Laboratory, Hefei 230038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,26]]},"reference":[{"key":"ref_1","unstructured":"Chinese Pharmacopoeia Commission (2015). 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