{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:01:56Z","timestamp":1762077716597,"version":"build-2065373602"},"reference-count":18,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2020-795","13.1902.21.0027","RF-190220X0027"],"award-info":[{"award-number":["075-15-2020-795","13.1902.21.0027","RF-190220X0027"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The study of the molecular mechanisms of the pathogenesis of Alzheimer\u2019s disease (AD) is extremely important for identifying potential therapeutic targets as well as early markers. In this regard, the study of the role of post-translational modifications (PTMs) of \u03b2-amyloid (A\u03b2) peptides is of particular relevance. Serine-8 phosphorylated forms (pSer8-A\u03b2) have been shown to have an increased aggregation capacity and may reflect the severity of amyloidosis. Here, an approach for quantitative assessment of pSer8-A\u03b2 based on matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is proposed. The relative fraction of pSer8-A\u03b2 was estimated in the total A\u03b2-pool with a detection limit of 1 fmol for pSer8-A\u03b2 (1\u201316) and an accuracy of 2% for measurements in the reflectron mode. The sensitivity of the developed method is suitable for determining the proportion of phosphorylated peptides in biological samples.<\/jats:p>","DOI":"10.3390\/molecules27238406","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T05:03:18Z","timestamp":1669870998000},"page":"8406","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Quantitative Assessment of Serine-8 Phosphorylated \u03b2-Amyloid Using MALDI-TOF Mass Spectrometry"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8462-9302","authenticated-orcid":false,"given":"Andrey A.","family":"Kuzin","sequence":"first","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galina S.","family":"Stupnikova","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6513-8009","authenticated-orcid":false,"given":"Polina A.","family":"Strelnikova","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Science, 119334 Moscow, Russia"},{"name":"Skolkovo Institute of Science and Technology, 121205 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ksenia V.","family":"Danichkina","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8177-7449","authenticated-orcid":false,"given":"Maria I.","family":"Indeykina","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Science, 119334 Moscow, Russia"},{"name":"Engelhardt Institute of Molecular Biology, 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9622-3457","authenticated-orcid":false,"given":"Stanislav I.","family":"Pekov","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia"},{"name":"Skolkovo Institute of Science and Technology, 121205 Moscow, Russia"},{"name":"Engelhardt Institute of Molecular Biology, 119991 Moscow, Russia"},{"name":"Siberian State Medical University, 634050 Tomsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor A.","family":"Popov","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia"},{"name":"Siberian State Medical University, 634050 Tomsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/alz.12328","article-title":"2021 Alzheimer\u2019s Disease Facts and Figures","volume":"17","author":"Gaugler","year":"2021","journal-title":"Alzheimer\u2019s Dement."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jalz.2012.11.007","article-title":"The Global Prevalence of Dementia: A Systematic Review and Metaanalysis","volume":"9","author":"Prince","year":"2013","journal-title":"Alzheimer\u2019s Dement."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1038\/aps.2017.28","article-title":"Amyloid Beta: Structure, Biology and Structure-Based Therapeutic Development","volume":"38","author":"Chen","year":"2017","journal-title":"Acta Pharmacol. 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Rev., e21775.","DOI":"10.1002\/mas.21775"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2255","DOI":"10.1038\/emboj.2011.138","article-title":"Extracellular Phosphorylation of the Amyloid \u03b2 2-Peptide Promotes Formation of Toxic Aggregates during the Pathogenesis of Alzheimer\u2019s Disease","volume":"30","author":"Kumar","year":"2011","journal-title":"EMBO J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/s00401-013-1107-8","article-title":"Early Intraneuronal Accumulation and Increased Aggregation of Phosphorylated Abeta in a Mouse Model of Alzheimer\u2019s Disease","volume":"125","author":"Kumar","year":"2013","journal-title":"Acta Neuropathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1093\/brain\/awt362","article-title":"Biochemical Stages of Amyloid-\u03b2 Peptide Aggregation and Accumulation in the Human Brain and Their Association with Symptomatic and Pathologically Preclinical Alzheimer\u2019s Disease","volume":"137","author":"Kosterin","year":"2014","journal-title":"Brain"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1134\/S0026893314040098","article-title":"Estimation of Phosphorylation Level of Amyloid-Beta Isolated from Human Blood Plasma: Ultrahigh-Resolution Mass Spectrometry","volume":"48","author":"Popov","year":"2014","journal-title":"Mol. 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