{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T02:18:07Z","timestamp":1785809887305,"version":"3.56.0"},"reference-count":42,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T00:00:00Z","timestamp":1670371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["21977042"],"award-info":[{"award-number":["21977042"]}]},{"name":"National Natural Science Foundation of China","award":["21977017"],"award-info":[{"award-number":["21977017"]}]},{"name":"National Natural Science Foundation of China","award":["32171270"],"award-info":[{"award-number":["32171270"]}]},{"name":"National Natural Science Foundation of China","award":["2020TD67"],"award-info":[{"award-number":["2020TD67"]}]},{"name":"Central Public-Interest Scientific Institution Basal Research Fund","award":["21977042"],"award-info":[{"award-number":["21977042"]}]},{"name":"Central Public-Interest Scientific Institution Basal Research Fund","award":["21977017"],"award-info":[{"award-number":["21977017"]}]},{"name":"Central Public-Interest Scientific Institution Basal Research Fund","award":["32171270"],"award-info":[{"award-number":["32171270"]}]},{"name":"Central Public-Interest Scientific Institution Basal Research Fund","award":["2020TD67"],"award-info":[{"award-number":["2020TD67"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Tetracyclines are one class of widely used antibiotics. Meanwhile, due to abuse and improper disposal, they are often detected in wastewater, which causes a series of environmental problems and poses a threat to human health and safety. As an efficient and environmentally friendly method, enzymatic catalysis has attracted much attention. In previous studies, we have designed an efficient peroxidase (F43Y\/P88W\/F138W Mb, termed YWW Mb) based on the protein scaffold of myoglobin (Mb), an O2 carrier, by modifying the heme active center and introducing two Trp residues. In this study, we further applied it to degrade the tetracycline antibiotics. Both UV-Vis and HPLC studies showed that the triple mutant YWW Mb was able to catalyze the degradation of tetracycline, oxytetracycline, doxycycline, and chlortetracycline effectively, with a degradation rate of ~100%, ~98%, ~94%, and ~90%, respectively, within 5 min by using H2O2 as an oxidant. These activities are much higher than those of wild-type Mb and other heme enzymes such as manganese peroxidase. As further analyzed by UPLC-ESI-MS, we identified multiple degradation products and thus proposed possible degradation mechanisms. In addition, the toxicity of the products was analyzed by using in vitro antibacterial experiments of E. coli. Therefore, this study indicates that the engineered heme enzyme has potential applications for environmental remediation by degradation of tetracycline antibiotics.<\/jats:p>","DOI":"10.3390\/molecules27248660","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T02:51:56Z","timestamp":1670467916000},"page":"8660","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Efficient Degradation of Tetracycline Antibiotics by Engineered Myoglobin with High Peroxidase Activity"],"prefix":"10.3390","volume":"27","author":[{"given":"Guang-Rong","family":"Wu","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Juan","family":"Sun","sequence":"additional","affiliation":[{"name":"Hengyang Medical School, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8069-9717","authenticated-orcid":false,"given":"Jia-Kun","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Sustainable Development of Polar Fisheries, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Qin","family":"Gao","sequence":"additional","affiliation":[{"name":"Hengyang Medical School, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Shi","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Chemistry & Institute of Biomedical Science, Fudan University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2457-0871","authenticated-orcid":false,"given":"Ying-Wu","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China"},{"name":"Hengyang Medical School, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"141975","DOI":"10.1016\/j.scitotenv.2020.141975","article-title":"Occurrence, fate, and risk assessment of typical tetracycline antibiotics in the aquatic environment: A review","volume":"753","author":"Xu","year":"2021","journal-title":"Sci. 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