{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T23:56:56Z","timestamp":1788479816682,"version":"build-2803163510"},"reference-count":63,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,10]],"date-time":"2022-12-10T00:00:00Z","timestamp":1670630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Infrared spectroscopy measurements were performed on Lysozyme aqueous solutions also in the presence of PolyEthylene Glycol (PEG 400) as a function of an increasing temperature from T = 27 \u00b0C to 90 \u00b0C, and, successively in sequence, by decreasing temperatures from T = 90 \u00b0C to 27 \u00b0C. Data were analyzed by evaluating the spectral difference with respect to the initial spectrum collected at 27 \u00b0C. This procedure allows to quantitatively evaluate the thermal restraint related to the thermal scan from T = 27 \u00b0C to 90 \u00b0C, as well as to introduce a spectral resilience concerning the entire increasing and decreasing thermal paths which allow to highlight the bioprotectant effectiveness of low molecular weight PEG. In particular, the main purpose of the present work is to highlight the effects of a thermal treatment on a mixture of Lysozyme\/water and of Lysozyme\/water\/PEG 400 during an increasing temperature scan, and then after a successive decreasing temperature scan, in order to highlight the bioprotectant role of PEG 400. On that score, an evaluation of the spectral distances of the registered spectra as a function of increasing and decreasing temperatures has been performed and analyzed.<\/jats:p>","DOI":"10.3390\/molecules27248760","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T04:34:20Z","timestamp":1670819660000},"page":"8760","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Multiscale Spectral Analysis on Lysozyme Aqueous Solutions in the Presence of PolyEthyleneGlycol"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2417-0998","authenticated-orcid":false,"given":"Maria Teresa","family":"Caccamo","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Matematiche e Informatiche, Scienze Fisiche e Scienze Della Terra, Universit\u00e0 degli Studi di Messina, Viale Ferdinando Stagno D\u2019Alcontres 31, 98166 Messina, Italy"},{"name":"Consorzio Interuniversitario Scienze Fisiche Applicate (CISFA), Viale Ferdinando Stagno d\u2019Alcontres 31, 98166 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1333-909X","authenticated-orcid":false,"given":"Salvatore","family":"Magaz\u00f9","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Matematiche e Informatiche, Scienze Fisiche e Scienze Della Terra, Universit\u00e0 degli Studi di Messina, Viale Ferdinando Stagno D\u2019Alcontres 31, 98166 Messina, Italy"},{"name":"Consorzio Interuniversitario Scienze Fisiche Applicate (CISFA), Viale Ferdinando Stagno d\u2019Alcontres 31, 98166 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Lane, M.E., and Moore, D.J. 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