{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T05:41:10Z","timestamp":1780378870804,"version":"3.54.1"},"reference-count":114,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004512","name":"Cape Peninsula University of Technology, South Africa","doi-asserted-by":"publisher","award":["CPUT-RJ23"],"award-info":[{"award-number":["CPUT-RJ23"]}],"id":[{"id":"10.13039\/501100004512","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The present study investigated phenolic compounds, antioxidant, antidiabetic, and the anti-inflammatory potentials of methanolic and chloroform extracts of Eriocephalus africanus. The methanolic extract included, polyphenols (112 \u00b1 2.81 mg gallic acid equivalent (GAE)\/g), flavonols (76.12 \u00b1 7.95 mg quercetin equivalents (QE)\/g); antioxidant capacity (Ferric Reducing Antioxidant Power (FRAP) (752.64 \u00b1 89.0 \u03bcmol of ascorbic acid equivalents (AAE) per g dry weight (\u00b5mol AAE\/g), 2,2-dyphenyl-1-picrylhydrazyl (DPPH) (812.18 \u00b1 51.12 Trolox equivalents per gram of dry mass of plant extracts (\u03bcmol TE\/g), TEAC (631.63 \u00b1 17.42 \u00b5mol TE\/g)), while the chloroform extract included polyphenols (39.93 \u00b1 1.36 mg GAE\/g), flavonols (44.81 \u00b1 3.74 mg QE\/g); antioxidant capacity, DPPH (58.70 \u00b1 5.18 \u00b5mol TE\/g), TEAC (118.63 \u00b1 3.74 \u00b5mol TE\/g) and FRAP (107.10 \u00b1 2.41 \u00b5mol AAE\/g). The phytochemicals profiling performed by UPLC-ESI-QTOF-MS revealed some important polyphenols, predominantly flavonoids, that could be responsible for the antioxidant capacity and biological effects. Both extracts demonstrated a dose-dependent manner of the alpha-glucosidase inhibition with an IC50 between 125 and 250 \u03bcg\/mL for methanolic extract, while the chloroform extract was at 250 \u03bcg\/mL. In the L6 myoblasts and C3A hepatocytes, the methanolic extract slightly increased the utilization of glucose, and both extracts exhibited a dose-dependent increase in the glucose uptake in both cell types without significantly increasing the cytotoxicity. Furthermore, both extracts exhibited an anti-inflammatory potential and the findings from the present study could serve as a baseline for further research in the development of pharmaceutical agents.<\/jats:p>","DOI":"10.3390\/molecules27248912","type":"journal-article","created":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T03:43:49Z","timestamp":1671075829000},"page":"8912","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["UPLC-ESI-QTOF-MS Profiling of Phenolic Compounds from Eriocephalus africanus: In Vitro Antioxidant, Antidiabetic, and Anti-Inflammatory Potentials"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7211-714X","authenticated-orcid":false,"given":"Kunle","family":"Okaiyeto","sequence":"first","affiliation":[{"name":"Phytomedicine and Phytochemistry Group, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3700-3937","authenticated-orcid":false,"given":"Nasifu","family":"Kerebba","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Makerere University, Kampala P.O. Box 7062, Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7299-1128","authenticated-orcid":false,"given":"Oluwafemi O.","family":"Oguntibeju","sequence":"additional","affiliation":[{"name":"Phytomedicine and Phytochemistry Group, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.1007\/s13197-016-2228-6","article-title":"In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetagetin extracted from marigold (Tagetes erecta L.) inflorescence residues","volume":"53","author":"Wang","year":"2016","journal-title":"J. Food Sci. Technol."},{"key":"ref_2","unstructured":"International Diabetes Federation (2017). IDF Diabetes Atlas 2017, International Diabetes Federation. [8th ed.]."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hoyas, I., and Leon-Sanz, M. (2019). Nutritional Challenges in Metabolic Syndrome. J. Clin. Med., 8.","DOI":"10.3390\/jcm8091301"},{"key":"ref_4","first-page":"45","article-title":"Type 2 diabetes mellitus, oxidative stress and inflammation: Examining the links","volume":"11","author":"Oguntibeju","year":"2019","journal-title":"Int. J. Physiol. Pathophysiol. Pharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.metabol.2017.08.010","article-title":"Pharmacotherapy of type 2 diabetes: An update","volume":"78","author":"Upadhyay","year":"2018","journal-title":"Metabolism"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1002\/mnfr.201300223","article-title":"Overview of food products and dietary constituents with antidiabetic properties and their putative mechanisms of action: A natural approach to complement pharmacotherapy in the management of diabetes","volume":"58","author":"Lacroix","year":"2013","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Hossain, M.K., Dayem, A.A., Han, J., Yin, Y., Kim, K., Saha, S.K., Yang, G.-M., Choi, H.Y., and Cho, S.-G. (2016). Molecular Mechanisms of the Anti-Obesity and Anti-Diabetic Properties of Flavonoids. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17040569"},{"key":"ref_8","first-page":"E906","article-title":"Effect of lowering postprandial hyperglycemia on insulin secretion in older people with impaired glucose tolerance","volume":"287","author":"Chang","year":"2004","journal-title":"Am. J. Physiol. Metab."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"546","DOI":"10.4103\/2230-8210.183480","article-title":"Microvasular and macrovascular complications in diabetes mellitus: Distinct or continuum?","volume":"20","author":"Chawla","year":"2016","journal-title":"Ind. J. Endocrinol. Metab."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1080\/01635581.2017.1267777","article-title":"Oxidative stress and carcinogenesis: Potential of phy-tochemicals in breast cancer therapy","volume":"69","author":"Forcados","year":"2017","journal-title":"Nutr. Cancer."},{"key":"ref_11","first-page":"15","article-title":"Role of Plant-Derived Polyphenols in Reducing Oxidative Stress-Mediated Diabetic Complications","volume":"5","author":"Bhattacharya","year":"2018","journal-title":"React. Oxyg. Species"},{"key":"ref_12","first-page":"53","article-title":"Oxidative DNA damage: Antioxidant response in postprandial hyperglycaemia in type 2 diabetes mellitus","volume":"11","author":"Jelinek","year":"2011","journal-title":"Brit. J. Diab. Vasc. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1080\/13510002.2016.1215643","article-title":"Hyperglycaemia, oxidative stress and inflammation markers","volume":"22","author":"Butkowski","year":"2017","journal-title":"Redox Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3577","DOI":"10.1002\/jcb.26097","article-title":"Mechanism of generation of oxidative stress and pathophysiology of type 2 diabetes mellitus: How are they interlinked?","volume":"118","author":"Rehman","year":"2017","journal-title":"J. Cell Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1097\/MED.0b013e32833bf6dc","article-title":"Role of IL-1beta in type 2 diabetes","volume":"17","author":"Dinarello","year":"2010","journal-title":"Curr. Opin. Endocrinol. Diabetes Obes."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.freeradbiomed.2010.12.006","article-title":"Oxidative stress, insulin signaling, and diabetes","volume":"50","author":"Rains","year":"2011","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1016\/j.cmet.2013.05.001","article-title":"Inflammation in Obesity and Diabetes: Islet Dysfunction and Therapeutic Opportunity","volume":"17","author":"Donath","year":"2013","journal-title":"Cell Metab."},{"key":"ref_18","first-page":"49","article-title":"Free Radicals and the Role of Plant Phytochemicals as Antioxidants Against Oxidative Stress-Related Diseases. Phytochemicals: Source of Antioxidants and Role in Disease Prevention","volume":"7","author":"Engwa","year":"2018","journal-title":"BoD-Books Demand"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1089\/ars.2016.6755","article-title":"The Role of Oxidative Stress and Hypoxia in Pancreatic Beta-Cell Dysfunction in Diabetes Mellitus","volume":"26","author":"Gerber","year":"2017","journal-title":"Antioxid. Redox Signal."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1002\/fft2.15","article-title":"Dietary polyphenols as antidiabetic agents: Advances and opportunities","volume":"1","author":"Sun","year":"2020","journal-title":"Food Front."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"104447","DOI":"10.1016\/j.arabjc.2022.104447","article-title":"UPLC-ESI-QTOF-MS phenolic compounds identification and quantification from ethanolic extract of Myrtus communis \u2018Variegatha\u2019: In vitro antioxidant and antidiabetic potentials","volume":"16","author":"Okaiyeto","year":"2022","journal-title":"Arab. J. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1356893","DOI":"10.1155\/2020\/1356893","article-title":"Antioxidant Effects and Mechanisms of Medicinal Plants and Their Bioactive Compounds for the Prevention and Treatment of Type 2 Diabetes: An Updated Review","volume":"2020","author":"Unuofin","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.pharmthera.2016.06.005","article-title":"Phytochemicals in regulating fatty acid \u03b2-oxidation: Potential underlying mechanisms and their involvement in obesity and weight loss","volume":"165","author":"Rupasinghe","year":"2016","journal-title":"Pharmacol. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Olvera-Sandoval, C., Fabela-Illescas, H.E., Fern\u00e1ndez-Mart\u00ednez, E., Ortiz-Rodr\u00edguez, M.A., Cari\u00f1o-Cort\u00e9s, R., Ariza-Ortega, J.A., Hern\u00e1ndez-Gonz\u00e1lez, J.C., Olivo, D., Valadez-Vega, C., and Belefant-Miller, H. (2022). Potential Mechanisms of the Improvement of Glucose Homeostasis in Type 2 Diabetes by Pomegranate Juice. Antioxidants, 11.","DOI":"10.3390\/antiox11030553"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Mahnashi, M.H., Alqahtani, Y.S., Alyami, B.A., Alqarni, A.O., Alqahl, S.A., Ullah, F., Sadiq, A., Zeb, A., Ghufran, M., and Kuraev, A. (2022). HPLC-DAD phenolics analysis, \u03b1-glucosidase, \u03b1-amylase inhibitory, molecular docking and nutritional profiles of Persicaria hydropiper L. BMC Complement. Altern. Med., 22.","DOI":"10.1186\/s12906-022-03510-7"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Tran, N., Pham, B., and Le, L. (2020). Bioactive Compounds in Anti-Diabetic Plants: From Herbal Medicine to Modern Drug Discovery. Biology, 9.","DOI":"10.3390\/biology9090252"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.arabjc.2015.04.018","article-title":"Characterization of phenolic constituents and evaluation of antioxidant properties of leaves and stems of Eriocephalus africanus","volume":"11","author":"Catarino","year":"2018","journal-title":"Arab. J. Chem."},{"key":"ref_28","unstructured":"van Wyk, B.E., van Oudtshoorn, B., and Gericke, N. (1997). Medicinal Plants of South Africa, Briza Publications."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3394","DOI":"10.1080\/14786419.2019.1566824","article-title":"Toxicity effects of Eriocephalus africanus L. leaf essential oil against some molecularly identified phytopathogenic bacterial strains","volume":"34","author":"Behiry","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4173","DOI":"10.1080\/14786419.2020.1744138","article-title":"Phytochemical constituents and in vitro anticancer screening of isolated compounds from Eriocephalus africanus","volume":"35","author":"Magura","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Khalil, N., Elhady, S.S., Diri, R.M., Fekry, M.I., Bishr, M., Salama, O., and El-Zalabani, S.M. (2022). Salicylic Acid Spraying Affects Sec-ondary Metabolites and Radical Scavenging Capacity of Drought-Stressed Eriocephalus africanus L. Agronomy, 12.","DOI":"10.3390\/agronomy12102278"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2568","DOI":"10.1007\/s13197-012-0754-4","article-title":"Influence of different solvents in extraction of phenolic compounds from vegetable residues and their evaluation as natural sources of antioxidants","volume":"51","author":"Babbar","year":"2014","journal-title":"J. Food Sci. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1111\/nph.13325","article-title":"Secondary metabolites in plant innate immunity: Conserved function of di-vergent chemicals","volume":"206","author":"Piasecka","year":"2015","journal-title":"New Phytol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s40659-019-0246-3","article-title":"Stress and defense responses in plant secondary metabolites production","volume":"52","author":"Isah","year":"2019","journal-title":"Biol. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Yang, L., Wen, K.-S., Ruan, X., Zhao, Y.-X., Wei, F., and Wang, Q. (2018). Response of Plant Secondary Metabolites to Environmental Factors. Molecules, 23.","DOI":"10.3390\/molecules23040762"},{"key":"ref_36","first-page":"25","article-title":"Flavonoids as natural phenolic compounds and their role in therapeutics: An overview. Futur","volume":"7","author":"Mutha","year":"2021","journal-title":"J. Pharm. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"153081","DOI":"10.1016\/j.phymed.2019.153081","article-title":"Comparison between tumors in plants and human beings: Mechanisms of tumor development and therapy with secondary plant metabolites","volume":"64","author":"Ullrich","year":"2019","journal-title":"Phytomedicine"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.semcancer.2020.02.007","article-title":"Modulation of dysregulated cancer metabolism by plant secondary metabolites: A mechanistic review. Semin","volume":"80","author":"Fakhri","year":"2020","journal-title":"Cancer Biol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Cosme, P., Rodr\u00edguez, A.B., Espino, J., and Garrido, M. (2020). Plant Phenolics: Bioavailability as a Key Determinant of Their Potential Health-Promoting Applications. Antioxidants, 9.","DOI":"10.3390\/antiox9121263"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"103273","DOI":"10.1016\/j.sjbs.2022.03.023","article-title":"Potential of natural phenolic antioxidant compounds from Bersama abyssinica (Meliathacea) for treatment of chronic diseases","volume":"29","author":"Zekeya","year":"2022","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s40816-021-00293-1","article-title":"Root extracts of Anacardium occidentale reduce hyperglycemia and oxidative stress in vitro","volume":"7","author":"Archana","year":"2021","journal-title":"Clin. Phytosci."},{"key":"ref_42","unstructured":"Harborne, J.B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Chapman and Hall."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e202104406","DOI":"10.1002\/slct.202104406","article-title":"UHPLC-ESI-QTOF-MS\/MS characterisation of phenolic compounds from Tithonia diversifolia (Hemsl.) A. gray and antioxidant activity","volume":"7","author":"Kerebba","year":"2022","journal-title":"ChemistrySelect"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/0891-5849(95)02227-9","article-title":"Structure-antioxidant activity relationships of flavonoids and phenolic acids","volume":"20","author":"Miller","year":"1996","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_45","unstructured":"Magura, J. (2020). The Effect of Isolated and Nanoencapsulated Flavonoids from Eriocephalus africanus on Apoptotic Factors and Microrna Expression in Cancer. [Doctoral Dissertation, University of KwaZulu-Natal]."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3821","DOI":"10.1021\/jf050046h","article-title":"Discriminating between the six isomers of dicaffeoylquinic acid by LC-MS","volume":"53","author":"Clifford","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1039\/b802662a","article-title":"Dietary phenolics: Chemistry, bioavailability and effects on health","volume":"26","author":"Crozier","year":"2009","journal-title":"Nat. Prod. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2533","DOI":"10.1002\/rcm.4662","article-title":"Rapid screening and identification of caffeic acid and its esters in Erigeron breviscapus by ultra-performance liquid chromatography\/tandem mass spectrometry","volume":"24","author":"Liao","year":"2010","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1002\/jms.600","article-title":"Mass spectrometry of 1,3-and 1,5-dicaffeoylquinic acids","volume":"39","author":"Schram","year":"2004","journal-title":"J. Mass Spectrom."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3","DOI":"10.17756\/jfcn.2015-001","article-title":"Chlorogenic Acid Profiles and Antioxidant Potentials of 17 Sweet Potato Varieties Cultivated in Korea: Impact of Extraction Condition and Classification by Hierarchical Clustering Analysis","volume":"1","author":"Park","year":"2015","journal-title":"J. Food Chem. Nanotechnol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"593","DOI":"10.3390\/12030593","article-title":"Characterization of Flavonoid Subgroups and Hydroxy Substitution by HPLC-MS\/MS","volume":"12","author":"Tsimogiannis","year":"2007","journal-title":"Molecules"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4587","DOI":"10.1016\/j.chroma.2010.04.076","article-title":"A practical strategy for the characterization of coumarins in Radix Glehniae by liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry","volume":"1217","author":"Yang","year":"2010","journal-title":"J. Chromatogr. A"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.21273\/HORTSCI16139-21","article-title":"Analysis of phenolic compounds and some important analytical properties in selected apricot genotypes","volume":"56","author":"Kiss","year":"2021","journal-title":"HortScience"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Bhatia, A., Singh, B., Arora, R., and Arora, S. (2019). In vitro evaluation of the \u03b1-glucosidase inhibitory potential of methanolic extracts of traditionally used antidiabetic plants. BMC Complement. Altern. Med., 19.","DOI":"10.1186\/s12906-019-2482-z"},{"key":"ref_55","first-page":"128","article-title":"In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts","volume":"3","author":"Nair","year":"2013","journal-title":"Eur. J. Exp. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1038\/s41598-022-07015-8","article-title":"Antidiabetic activity of avocado seeds (Persea americana Mill.) in diabetic rats via activation of PI3K\/AKT signaling pathway","volume":"12","author":"Ojo","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Daou, M., Elnaker, N.A., Ochsenk\u00fchn, M.A., Amin, S.A., Yousef, A.F., and Yousef, L.F. (2022). In vitro \u03b1-glucosidase inhibitory activity of Tamarix nilotica shoot extracts and fractions. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0264969"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Jadalla, B.M.I.S., Moser, J.J., Sharma, R., Etsassala, N.G.E.R., Egieyeh, S.A., Badmus, J.A., Marnewick, J.L., Beukes, D., Cupido, C.N., and Hussein, A.A. (2022). In Vitro Alpha-Glucosidase and Alpha-Amylase Inhibitory Activities and Antioxidant Capacity of Helichrysum cymosum and Helichrysum pandurifolium Schrank Constituents. Separations, 9.","DOI":"10.3390\/separations9080190"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1002\/jsfa.10771","article-title":"Polyphenol fingerprinting and hypoglycemic attributes of optimized Cycas circinalis leaf extracts","volume":"101","author":"Arshad","year":"2021","journal-title":"J. Sci. Food Agric."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e7022","DOI":"10.7717\/peerj.7022","article-title":"Antioxidant activity, \u03b1-glucosidase inhibition and phytochemical profiling of Hyophorbela genicaulis leaf extracts","volume":"7","author":"William","year":"2019","journal-title":"PeerJ"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Niaz, A., Adnan, A., Bashir, R., Mumtaz, M.W., Raza, S.A., Rashid, U., Tan, C.P., and Tan, T.B. (2021). The In Vitro \u03b1-Glucosidase Inhibition Activity of Various Solvent Fractions of Tamarix dioica and 1H-NMR Based Metabolite Identification and Molecular Docking Analysis. Plants, 10.","DOI":"10.3390\/plants10061128"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3530","DOI":"10.1038\/s41598-020-60076-5","article-title":"UHPLC-QTOF-MS\/MS based phytochemical characterization and anti-hyperglycemic prospective of hydro-ethanolic leaf extract of Butea monosperma","volume":"10","author":"Farooq","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_63","first-page":"2737","article-title":"Antioxidant activity, hypoglycemic potential and metabolite profiling of Hyophorbe indica leaf extract","volume":"31","author":"William","year":"2018","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"315","DOI":"10.2174\/138955710791331007","article-title":"Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypo-glycaemic potential in the treatment of diabetes: An update","volume":"4","author":"Tundis","year":"2010","journal-title":"Mini Rev. Med. Chem."},{"key":"ref_65","first-page":"S11","article-title":"Structural Studies of the Intestinal \u03b1-Glucosidases, Maltase-glucoamylase and Sucrase-isomaltase","volume":"66","author":"Rose","year":"2018","journal-title":"JPGN"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1111\/jdi.12358","article-title":"Peptide modulators of alpha-glucosidase","volume":"6","author":"Roskar","year":"2015","journal-title":"J. Diabetes Investig."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e11058","DOI":"10.1016\/j.heliyon.2022.e11058","article-title":"Antioxidant potential and in vitro inhibition of starch digestion of flavonoids from Crataegus pinnatifida","volume":"8","author":"Huang","year":"2022","journal-title":"Heliyon"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1080\/10942912.2018.1499112","article-title":"Metabolite profiling and inhibitory properties of leaf extracts of Ficus benjamina towards \u03b1-glucosidase and \u03b1-amylase","volume":"21","author":"Mumtaz","year":"2018","journal-title":"Int. J. Food Prop."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1080\/14756366.2017.1368503","article-title":"\u03b1-Glucosidase inhibition by flavonoids: An in vitro and in silico structure\u2013activity relationship study","volume":"32","author":"Freitas","year":"2017","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s13300-018-0554-6","article-title":"Behavioral Strategies to Lower Post-prandial Glucose in Those with Type 2 Diabetes May Also Lower Risk of Coronary Heart Disease","volume":"10","author":"Cox","year":"2019","journal-title":"Diabetes Ther."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.lwt.2011.10.011","article-title":"Anti-diabetic activities of phenolic compounds in muscadine against alpha-glucosidase and pancreatic lipase","volume":"46","author":"You","year":"2012","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1021\/np300204p","article-title":"\u03b1-Glucosidase inhibitors from Brickellia cavanillesii","volume":"75","author":"Bye","year":"2012","journal-title":"J. Nat. Prod."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s11418-008-0244-1","article-title":"Antioxidant constituents in the dayflower (Commelina communis L.) and their \u03b1-glucosidase-inhibitory activity","volume":"62","author":"Shibano","year":"2008","journal-title":"J. Nat. Med."},{"key":"ref_74","first-page":"796","article-title":"Effect of different phenolic compounds on \u03b1-amylase activity: Screening by microplate-reader based kinetic assay","volume":"60","author":"Funke","year":"2005","journal-title":"Pharmazie"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"2458","DOI":"10.1271\/bbb.64.2458","article-title":"Inhibition of Alpha-glucosidase and Amylase by Luteolin, a Flavonoid","volume":"64","author":"Kim","year":"2000","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"7652","DOI":"10.1021\/jf300601z","article-title":"Effect of Eriodictyol on Glucose Uptake and Insulin Resistance in Vitro","volume":"60","author":"Zhang","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1016\/j.bmcl.2013.12.098","article-title":"Rat intestinal sucrase inhibition of constituents from the roots of Rosa rugosa Thunb","volume":"24","author":"Thao","year":"2014","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1007\/s10600-009-9289-y","article-title":"\u03b1-glucosidase inhibitory constituents from Toona sinensis","volume":"45","author":"Zhao","year":"2009","journal-title":"Chem. Nat. Compd."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.1016\/j.phytochem.2007.02.020","article-title":"\u03b1-Glucosidase inhibitory constituents from stem bark of Terminalia superba (Combretaceae)","volume":"68","author":"Wansi","year":"2007","journal-title":"Phytochemistry"},{"key":"ref_80","first-page":"609","article-title":"Flavonoids from Salvia chloroleuca with \u03b1-amylsae and \u03b1-glucosidase inhibitory effect","volume":"14","author":"Asghari","year":"2015","journal-title":"Iran. J. Pharm. Res. IJPR"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.2337\/diabetes.52.5.1066","article-title":"5-Amino-Imidazole Carboxamide Riboside Increases Glucose Transport and Cell-Surface GLUT4 Content in Skeletal Muscle from Subjects with Type 2 Diabetes","volume":"52","author":"Koistinen","year":"2003","journal-title":"Diabetes"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.4314\/ahs.v19i2.47","article-title":"Annona stenophylla aqueous extract stimulate glucose uptake in established C2Cl2 muscle cell lines","volume":"19","author":"Taderera","year":"2019","journal-title":"Afr. Health Sci."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Hasan, M., Ahmed, Q.U., Soad, S.Z.M., Latip, J., Taher, M., Syafiq, T.M.F., Sarian, M.N., Alhassan, A.M., and Zakaria, Z.A. (2017). Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cells. BMC Complement. Altern. Med., 17.","DOI":"10.1186\/s12906-017-1929-3"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s11892-019-1194-6","article-title":"Alterations in Beta Cell Identity in Type 1 and Type 2 Diabetes","volume":"19","author":"Moin","year":"2019","journal-title":"Curr. Diabetes Rep."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"162","DOI":"10.17305\/bjbms.2016.919","article-title":"Apoptosis in pancreatic \u03b2-islet cells in Type 2 diabetes","volume":"16","author":"Tomita","year":"2016","journal-title":"Bosn. J. Basic Med. Sci."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"bvab128","DOI":"10.1210\/jendso\/bvab128","article-title":"Revisiting Regulators of Human \u03b2-cell Mass to Achieve \u03b2-cell\u2013centric Approach Toward Type 2 Diabetes","volume":"5","author":"Sasaki","year":"2021","journal-title":"J. Endocr. Soc."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1007\/s10495-009-0339-5","article-title":"Beta cell apoptosis in diabetes","volume":"14","author":"Thomas","year":"2009","journal-title":"Apoptosis"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1039\/D0FO02611E","article-title":"Comprehensive in vitro antidiabetic screening of Aspalathus linearis using a target-directed screening platform and cellomics","volume":"12","author":"Pringle","year":"2020","journal-title":"Food Funct."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jep.2015.05.003","article-title":"An extract of Artemisia dracunculus L. stimulates insulin secretion from \u03b2 cells, activates AMPK and suppresses inflammation","volume":"170","author":"Aggarwal","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"112043","DOI":"10.1016\/j.jep.2019.112043","article-title":"Centaurium erythraea extract improves survival and functionality of pancreatic beta-cells in diabetes through multiple routes of action","volume":"242","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"629863","DOI":"10.1155\/2015\/629863","article-title":"Plant-Derived Compounds Targeting Pancreatic Beta Cells for the Treatment of Diabetes","volume":"2015","author":"Oh","year":"2015","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1007\/s42399-021-01034-8","article-title":"Botanical Interventions to Improve Glucose Control and Options for Diabetes Therapy","volume":"3","author":"Smoak","year":"2021","journal-title":"SN Compr. Clin. Med."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s13065-018-0445-y","article-title":"Inhibitory effects against \u03b1-glucosidase and \u03b1-amylase of the flavonoids-rich extract from Scutellaria baicalensis shoots and interpretation of structure\u2013activity relationship of its eight flavonoids by a refined assign-score method","volume":"12","author":"Li","year":"2018","journal-title":"Chem. Central J."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"7220","DOI":"10.1021\/jf020615a","article-title":"Antioxidant and Prooxidant Effects of Phenolics on Pancreatic \u03b2-Cells in Vitro","volume":"50","author":"Lapidot","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1038\/s41591-019-0675-0","article-title":"Chronic inflammation in the etiology of disease across the life span","volume":"25","author":"Furman","year":"2019","journal-title":"Nat. Med."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"7204","DOI":"10.18632\/oncotarget.23208","article-title":"Inflammatory responses and inflammation-associated diseases in organs","volume":"9","author":"Chen","year":"2018","journal-title":"Oncotarget"},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Raziyeva, K., Kim, Y., Zharkinbekov, Z., Kassymbek, K., Jimi, S., and Saparov, A. (2021). Immunology of Acute and Chronic Wound Healing. Biomolecules, 11.","DOI":"10.3390\/biom11050700"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.sajb.2019.07.013","article-title":"Antioxidant, anti-inflammatory and wound healing properties of medicinal plant extracts used to treat wounds and dermatological disorders","volume":"126","author":"Ghuman","year":"2019","journal-title":"S. Afr. J. Bot."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1007\/s10495-009-0352-8","article-title":"Diabetes and apoptosis: Lipotoxicity","volume":"14","author":"Kusminski","year":"2009","journal-title":"Apoptosis"},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Ghemrawi, R., Battaglia-Hsu, S.-F., and Arnold, C. (2018). Endoplasmic Reticulum Stress in Metabolic Disorders. Cells, 7.","DOI":"10.20944\/preprints201805.0264.v1"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Okaiyeto, K., and Oguntibeju, O. (2021). African Herbal Medicines: Adverse Effects and Cytotoxic Potentials with Different Therapeutic Applications. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18115988"},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Sarbadhikary, P., and George, B.P. (2022). A Review on Traditionally Used African Medicinal Plant Annickia chlorantha, Its Phytochemistry, and Anticancer Potential. Plants, 11.","DOI":"10.3390\/plants11172293"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"e08397","DOI":"10.1016\/j.heliyon.2021.e08397","article-title":"Phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa","volume":"7","author":"Okafor","year":"2021","journal-title":"Heliyon"},{"key":"ref_104","unstructured":"Yermakov, A.I., Arasimov, V.V., and Yarosh, N.P. (1987). Methods of Biochemical Analysis of Plants, Agropromizdat."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolourization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The ferric reducing ability of plasma (FRAP) as a measure of \u201cantioxidant power\u201d: The FRAP assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Ngxabi, S., Jimoh, M.O., Kambizi, L., and Laubscher, C.P. (2021). Growth characteristics, phytochemical contents, and antioxidant capacity of Trachyandra ciliata (Lf) Kunth grown in hydroponics under varying degrees of salinity. Horticulturae, 7.","DOI":"10.3390\/horticulturae7080244"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.jep.2008.05.031","article-title":"Antidiabetic screening and scoring of 11 plants traditionally used in South Africa","volume":"119","author":"Roux","year":"2008","journal-title":"J. Ethnopharmacol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1177\/000456326900600108","article-title":"Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor","volume":"6","author":"Trinder","year":"1969","journal-title":"Ann. Clin. Biochem. Int. J. Biochem. Lab. Med."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/bjc.1987.190","article-title":"A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity","volume":"56","author":"Twentyman","year":"1987","journal-title":"Br. J. Cancer"},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Okaiyeto, K., Ojemaye, M.O., Hoppe, H., Mabinya, L.V., and Okoh, A.I. (2019). Phytofabrication of silver\/silver chloride nanoparticles using aqueous leaf extract of Oedera genistifolia: Characterization and antibacterial potential. Molecules, 24.","DOI":"10.3390\/molecules24234382"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"114596","DOI":"10.1016\/j.jep.2021.114596","article-title":"Exploring four south African croton species for potential anti-inflammatory properties: In vitro activity and toxicity risk as-sessment","volume":"282","author":"Rampa","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"10270","DOI":"10.1021\/acs.jafc.7b03942","article-title":"Analysis of Phenolic Compounds in Rooibos Tea (Aspalathus linearis) with a Comparison of Flavonoid-Based Compounds in Natural Populations of Plants from Different Regions","volume":"65","author":"Stander","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1002\/mas.21535","article-title":"Identification of small molecules using accurate mass MS\/MS search","volume":"37","author":"Kind","year":"2018","journal-title":"Mass Spectrom. Rev."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/24\/8912\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:41:46Z","timestamp":1760146906000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/27\/24\/8912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,15]]},"references-count":114,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["molecules27248912"],"URL":"https:\/\/doi.org\/10.3390\/molecules27248912","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,15]]}}}