{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T13:12:54Z","timestamp":1784121174848,"version":"3.55.0"},"reference-count":85,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T00:00:00Z","timestamp":1671148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan","award":["IRN \u2116 AP09259196"],"award-info":[{"award-number":["IRN \u2116 AP09259196"]}]},{"name":"Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan","award":["DS 503-07-01-38"],"award-info":[{"award-number":["DS 503-07-01-38"]}]},{"name":"Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan","award":["DS 24"],"award-info":[{"award-number":["DS 24"]}]},{"DOI":"10.13039\/501100014413","name":"statutory project of the University of Information Technology and Management in Rzeszow, Poland","doi-asserted-by":"publisher","award":["IRN \u2116 AP09259196"],"award-info":[{"award-number":["IRN \u2116 AP09259196"]}],"id":[{"id":"10.13039\/501100014413","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014413","name":"statutory project of the University of Information Technology and Management in Rzeszow, Poland","doi-asserted-by":"publisher","award":["DS 503-07-01-38"],"award-info":[{"award-number":["DS 503-07-01-38"]}],"id":[{"id":"10.13039\/501100014413","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014413","name":"statutory project of the University of Information Technology and Management in Rzeszow, Poland","doi-asserted-by":"publisher","award":["DS 24"],"award-info":[{"award-number":["DS 24"]}],"id":[{"id":"10.13039\/501100014413","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010621","name":"Medical University of Lublin","doi-asserted-by":"publisher","award":["IRN \u2116 AP09259196"],"award-info":[{"award-number":["IRN \u2116 AP09259196"]}],"id":[{"id":"10.13039\/501100010621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010621","name":"Medical University of Lublin","doi-asserted-by":"publisher","award":["DS 503-07-01-38"],"award-info":[{"award-number":["DS 503-07-01-38"]}],"id":[{"id":"10.13039\/501100010621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010621","name":"Medical University of Lublin","doi-asserted-by":"publisher","award":["DS 24"],"award-info":[{"award-number":["DS 24"]}],"id":[{"id":"10.13039\/501100010621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Ziziphora species (Lamiaceae) have been used in traditional medicine as sedatives, antiseptics, carminatives, or expectorants. Despite their common applications in phytotherapy, there is still lack of evidence about the composition of their extracts and its impact on biological properties of the plants. The aim of this study was to evaluate the content of Ziziphora bungeana, a less studied species growing in Kazakhstan, using HPLC-ESI-QTOF-MS\/MS instrumentation and to determine its antimicrobial, antioxidant, and cytotoxic activity together with inhibitory properties against tyrosinase and toxicity in erythrocyte lysis assay. Extracts from Z. bungeana were found to be sources of flavonoids, phenolic acids, organic acids, and terpenes that determined their antiradical activity. The minimum inhibitory concentrations of extracts were lower for Gram-positive bacteria (1.25\u201310 mg\/mL) than for Gram-negative bacteria and fungi (5\u201320 mg\/mL). The EC50 value calculated for antiradical activity ranged between 15.00 \u00b1 1.06 \u00b5g\/mL and 13.21 \u00b1 3.24 \u00b5g\/mL for ABTS and DPPH assays, respectively. Z. bungeana extracts were found to decrease the activity of tyrosinase by 50% (at 200 \u00b5g\/mL) similarly to kojic acid and were slightly cytotoxic for human melanoma A375 cell line (at 200 \u00b5g\/mL) with no effect on HaCaT keratinocytes. In the end, Z. bungeana did not reveal toxic effects in hemolytic assay as compared to the positive control Triton X-100. The performed tests show potential application of the plant in the treatment of infectious diseases, disorders caused by free radicals, and skin problems.<\/jats:p>","DOI":"10.3390\/molecules27248994","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T09:05:09Z","timestamp":1671440709000},"page":"8994","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Multidirectional Characterization of Phytochemical Profile and Health-Promoting Effects of Ziziphora bungeana Juz. Extracts"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3776-2004","authenticated-orcid":false,"given":"Karlygash","family":"Zhaparkulova","sequence":"first","affiliation":[{"name":"School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aigerim","family":"Karaubayeva","sequence":"additional","affiliation":[{"name":"School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuriyadda","family":"Sakipova","sequence":"additional","affiliation":[{"name":"School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2844-9876","authenticated-orcid":false,"given":"Anna","family":"Biernasiuk","sequence":"additional","affiliation":[{"name":"Chair and Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Lublin, 1, Chodzki str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5773-1849","authenticated-orcid":false,"given":"Katarzyna","family":"Gawe\u0142-B\u0119ben","sequence":"additional","affiliation":[{"name":"Department of Cosmetology, University of Information Technology and Management in Rzesz\u00f3w, 2 Sucharskiego str., 35-225 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4868-2381","authenticated-orcid":false,"given":"Tomasz","family":"Laskowski","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gda\u0144sk University of Technology, Gabriela Narutowicza Str. 11\/12, 80-233 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliya","family":"Kusniyeva","sequence":"additional","affiliation":[{"name":"School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azamat","family":"Omargali","sequence":"additional","affiliation":[{"name":"Edinburgh Dental Institute, The University of Edinburgh, Edinburgh EH8 9YL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tolkyn","family":"Bekezhanova","sequence":"additional","affiliation":[{"name":"School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliya","family":"Ibragimova","sequence":"additional","affiliation":[{"name":"School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galiya","family":"Ibadullayeva","sequence":"additional","affiliation":[{"name":"School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amangeldy","family":"Jakiyanov","sequence":"additional","affiliation":[{"name":"School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Czech","sequence":"additional","affiliation":[{"name":"Department of Cosmetology, University of Information Technology and Management in Rzesz\u00f3w, 2 Sucharskiego str., 35-225 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2338-8816","authenticated-orcid":false,"given":"Kuanysh","family":"Tastambek","sequence":"additional","affiliation":[{"name":"School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, Kazakhstan"},{"name":"Department of Fundamental Medicine, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan"},{"name":"Department of Chemical and Biochemical Engineering, Geology and Oil-Gas Business Institute Named after K. Turyssov, Institute of Chemical and Biological Technologies, Satbayev University, Almaty 050043, Kazakhstan"},{"name":"Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan 161200, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazimierz","family":"G\u0142owniak","sequence":"additional","affiliation":[{"name":"Department of Cosmetology, University of Information Technology and Management in Rzesz\u00f3w, 2 Sucharskiego str., 35-225 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1503-7634","authenticated-orcid":false,"given":"Anna","family":"Malm","sequence":"additional","affiliation":[{"name":"Chair and Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Lublin, 1, Chodzki str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7076-600X","authenticated-orcid":false,"given":"Wirginia","family":"Kukula-Koch","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Medical University of Lublin, 1, Chod\u017aki str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1007\/s13197-017-2806-2","article-title":"Chemical compositions, antioxidant and antimicrobial properties of Ziziphora clinopodioides Lam. essential oils collected from different parts of Iran","volume":"54","author":"Shahbazi","year":"2017","journal-title":"J. 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