{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:43:43Z","timestamp":1760147023448,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T00:00:00Z","timestamp":1672704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FAPESP","award":["2015\/25406-5","2021\/12071-6","10\/00528-7"],"award-info":[{"award-number":["2015\/25406-5","2021\/12071-6","10\/00528-7"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The interaction of DNA with different block copolymers, namely poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(acrylamide), i.e., (PTEA)-b-(PAm), and poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(ethylene oxide), i.e., (PTEA)-b-(PEO), was studied. The nature of the cationic block was maintained fixed (PTEA), whereas the neutral blocks contained varying amounts of acrylamide or (ethylene oxide) units. According to results from isothermal titration microcalorimetry measurements, the copolymers interaction with DNA is endothermic with an enthalpy around 4.0 kJ mol\u22121 of charges for (PTEA)-b-(PAm) and 5.5 kJ mol\u22121 of charges for (PTEA)-b-(PEO). The hydrodynamic diameters of (PTEA)-b-(PEO)\/DNA and (PTEA)-b-(PAm)\/DNA polyplexes prepared by titration were around 200 nm at charge ratio (Z+\/\u2212) &lt; 1. At Z+\/\u2212 close and above 1, the (PTEA)50-b-(PAm)50\/DNA and (PTEA)50-b-(PAm)200\/DNA polyplexes precipitated. Interestingly, (PTEA)50-b-(PAm)1000\/DNA polyplexes remained with a size of around 300 nm even after charge neutralization, probably due to the size of the neutral block. Conversely, for (PTEA)96-b-(PEO)100\/DNA polyplexes, the size distribution was broad, indicating a more heterogeneous system. Polyplexes were also prepared by direct mixture at Z+\/\u2212 of 2.0, and they displayed diameters around 120\u2013150 nm, remaining stable for more than 10 days. Direct and reverse titration experiments showed that the order of addition affects both the size and charge of the resulting polyplexes.<\/jats:p>","DOI":"10.3390\/molecules28010398","type":"journal-article","created":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T02:33:21Z","timestamp":1672713201000},"page":"398","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessing the Contribution of the Neutral Blocks in DNA\/Block-Copolymer Polyplexes: Poly(acrylamide) vs. Poly(ethylene Oxide)"],"prefix":"10.3390","volume":"28","author":[{"given":"Renata Mello","family":"Giona","sequence":"first","affiliation":[{"name":"LaMaFI\u2014Laborat\u00f3rio de Materiais e Fen\u00f4menos de Interface, Chemistry Department, Universidade Tecnol\u00f3gica Federal do Paran\u00e1 (UTFPR), Medianeira, Curitiba 85884-000, Paran\u00e1 (PR), Brazil"},{"name":"Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6154, Campinas 13083-970, S\u00e3o Paulo State (SP), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Let\u00edcia","family":"Vitorazi","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6154, Campinas 13083-970, S\u00e3o Paulo State (SP), Brazil"},{"name":"Laborat\u00f3rio de Materiais Polim\u00e9ricos, EEIMVR, Universidade Federal Fluminense, Volta Redonda 27255-125, Rio de Janeiro (RJ), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8049-3321","authenticated-orcid":false,"given":"Watson","family":"Loh","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6154, Campinas 13083-970, S\u00e3o Paulo State (SP), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3216","DOI":"10.1038\/s41467-022-30969-2","article-title":"Light-Activated Photodeformable Supramolecular Dissipative Self-Assemblies","volume":"13","author":"Chen","year":"2022","journal-title":"Nat. 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