{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T06:29:50Z","timestamp":1768285790868,"version":"3.49.0"},"reference-count":83,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T00:00:00Z","timestamp":1672704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Council for Scientific and Technological Development","award":["306339\/2020-0"],"award-info":[{"award-number":["306339\/2020-0"]}]},{"name":"National Council for Scientific and Technological Development","award":["303224\/2018-5"],"award-info":[{"award-number":["303224\/2018-5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Extending the range of use of the heterologous fibrin biopolymer, this pre-clinical study showed a new proportionality of its components directed to the formation of scaffold with a lower density of the resulting mesh to facilitate the infiltration of bone cells, and combined with therapy by laser photobiomodulation, in order to accelerate the repair process and decrease the morphofunctional recovery time. Thus, a transoperative protocol of laser photobiomodulation (L) was evaluated in critical bone defects filled with deproteinized bovine bone particles (P) associated with heterologous fibrin biopolymer (HF). The groups were: BCL (blood clot + laser); HF; HFL; PHF (P+HF); PHFL (P+HF+L). Microtomographically, bone volume (BV) at 14 days, was higher in the PHF and PHFL groups (10.45 \u00b1 3.31 mm3 and 9.94 \u00b1 1.51 mm3), significantly increasing in the BCL, HFL and PHFL groups. Histologically, in all experimental groups, the defects were not reestablished either in the external cortical bone or in the epidural, occurring only in partial bone repair. At 42 days, the bone area (BA) increased in all groups, being significantly higher in the laser-treated groups. The quantification of bone collagen fibers showed that the percentage of collagen fibers in the bone tissue was similar between the groups for each experimental period, but significantly higher at 42 days (35.71 \u00b1 6.89%) compared to 14 days (18.94 \u00b1 6.86%). It can be concluded that the results of the present study denote potential effects of laser radiation capable of inducing functional bone regeneration, through the synergistic combination of biomaterials and the new ratio of heterologous fibrin biopolymer components (1:1:1) was able to make the resulting fibrin mesh less dense and susceptible to cellular permeability. Thus, the best fibrinogen concentration should be evaluated to find the ideal heterologous fibrin scaffold.<\/jats:p>","DOI":"10.3390\/molecules28010407","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T02:54:55Z","timestamp":1672800895000},"page":"407","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Tissue Bioengineering with Fibrin Scaffolds and Deproteinized Bone Matrix Associated or Not with the Transoperative Laser Photobiomodulation Protocol"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2858-4717","authenticated-orcid":false,"given":"Karina Torres","family":"Pomini","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry (FOB\/USP), University of S\u00e3o Paulo, Bauru 17012-901, Brazil"},{"name":"Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Mar\u00edlia 17525-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9914-1262","authenticated-orcid":false,"given":"Daniela Vieira","family":"Buchaim","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Mar\u00edlia 17525-902, Brazil"},{"name":"Teaching and Research Coordination of the Medical School, University Center of Adamantina (UNIFAI), Adamantina 17800-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina Cestari","family":"Bighetti","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry (FOB\/USP), University of S\u00e3o Paulo, Bauru 17012-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul Latif","family":"Hamz\u00e9","sequence":"additional","affiliation":[{"name":"Medical School, University of Marilia (UNIMAR), Mar\u00edlia 17525-160, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Henrique Bertoni","family":"Reis","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry (FOB\/USP), University of S\u00e3o Paulo, Bauru 17012-901, Brazil"},{"name":"UNIMAR Beneficent Hospital (HBU), University of Marilia (UNIMAR), Mar\u00edlia 17525-160, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3051-737X","authenticated-orcid":false,"given":"Marco Antonio H\u00fangaro","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of S\u00e3o Paulo (FOB\/USP), Bauru 17012-901, 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Ferreira","family":"J\u00fanior","sequence":"additional","affiliation":[{"name":"Center for the Study of Venoms and Venomous Animals (CEVAP), S\u00e3o Paulo State University (University Estadual Paulista, UNESP), Botucatu 18610-307, Brazil"},{"name":"Graduate Programs in Tropical Diseases and Clinical Research, Botucatu Medical School (FMB), S\u00e3o Paulo State University, (UNESP\u2013University Estadual Paulista), Botucatu 18618-687, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre Teixeira","family":"de Souza","sequence":"additional","affiliation":[{"name":"Rector\/President, University Center of Adamantina (UNIFAI), Adamantina 17800-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0674-3759","authenticated-orcid":false,"given":"Paulo S\u00e9rgio","family":"da Silva Santos","sequence":"additional","affiliation":[{"name":"Department of Surgery, Stomatology, Pathology and Radiology, Bauru 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