{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:47:34Z","timestamp":1791384454318,"version":"4.3.3"},"reference-count":151,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,21]],"date-time":"2023-01-21T00:00:00Z","timestamp":1674259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hong Kong Research Grants Council","award":["PolyU 15300019"],"award-info":[{"award-number":["PolyU 15300019"]}]},{"name":"Hong Kong Research Grants Council","award":["15300520"],"award-info":[{"award-number":["15300520"]}]},{"name":"Hong Kong Research Grants Council","award":["P0043412"],"award-info":[{"award-number":["P0043412"]}]},{"name":"Hong Kong Research Grants Council","award":["P0043816"],"award-info":[{"award-number":["P0043816"]}]},{"name":"PolyU Postdoc Matching Fund Scheme","award":["PolyU 15300019"],"award-info":[{"award-number":["PolyU 15300019"]}]},{"name":"PolyU Postdoc Matching Fund Scheme","award":["15300520"],"award-info":[{"award-number":["15300520"]}]},{"name":"PolyU Postdoc Matching Fund Scheme","award":["P0043412"],"award-info":[{"award-number":["P0043412"]}]},{"name":"PolyU Postdoc Matching Fund Scheme","award":["P0043816"],"award-info":[{"award-number":["P0043816"]}]},{"DOI":"10.13039\/501100004377","name":"State Key Laboratory of Chemical Biology and Drug Discovery, and The Hong Kong Polytechnic University","doi-asserted-by":"publisher","award":["PolyU 15300019"],"award-info":[{"award-number":["PolyU 15300019"]}],"id":[{"id":"10.13039\/501100004377","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004377","name":"State Key Laboratory of Chemical Biology and Drug Discovery, and The Hong Kong Polytechnic University","doi-asserted-by":"publisher","award":["15300520"],"award-info":[{"award-number":["15300520"]}],"id":[{"id":"10.13039\/501100004377","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004377","name":"State Key Laboratory of Chemical Biology and Drug Discovery, and The Hong Kong Polytechnic University","doi-asserted-by":"publisher","award":["P0043412"],"award-info":[{"award-number":["P0043412"]}],"id":[{"id":"10.13039\/501100004377","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004377","name":"State Key Laboratory of Chemical Biology and Drug Discovery, and The Hong Kong Polytechnic University","doi-asserted-by":"publisher","award":["P0043816"],"award-info":[{"award-number":["P0043816"]}],"id":[{"id":"10.13039\/501100004377","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The demand for creation of protein diversity and regulation of protein function through native protein modification and post-translational modification has ignited the development of selective chemical modification methods for peptides and proteins. Chemical bioconjugation offers selective functionalization providing bioconjugates with desired properties and functions for diverse applications in chemical biology, medicine, and biomaterials. The amino group existing at the lysine residue and N-terminus of peptides and proteins has been extensively studied in bioconjugation because of its good nucleophilicity and high surface exposure. Herein, we review the development of chemical methods for modification of the amino groups on lysine residue and N-terminus featuring excellent selectivity, mild reaction conditions, short reaction time, high conversion, biocompatibility, and preservation of protein integrity. This review is organized based on the chemoselectivity and site-selectivity of the chemical bioconjugation reagents to the amino acid residues aiming to provide guidance for the selection of appropriate bioconjugation methods.<\/jats:p>","DOI":"10.3390\/molecules28031083","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T01:36:26Z","timestamp":1674437786000},"page":"1083","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":87,"title":["Development and Recent Advances in Lysine and N-Terminal Bioconjugation for Peptides and Proteins"],"prefix":"10.3390","volume":"28","author":[{"given":"Ajcharapan","family":"Tantipanjaporn","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hum, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1574-6860","authenticated-orcid":false,"given":"Man-Kin","family":"Wong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hum, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1038\/nchembio.720","article-title":"Choosing an effective protein bioconjugation strategy","volume":"7","author":"Stephanopoulos","year":"2011","journal-title":"Nat. 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