{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:08:34Z","timestamp":1790863714962,"version":"4.1.0"},"reference-count":40,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T00:00:00Z","timestamp":1674432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Artemisia annua L. is distributed throughout the world and it is an important medicinal plant in Korea to treat various human diseases. Recently, A. annua has also been considered to be an effective ethnobotanical drug against COVID-19. A. annua contains an appreciable amount of essential oil with different biological properties. However, the composition of essential oils in aromatic plants can be varied depending on several factors, including geographic, genetic, ecological, etc. Hence, the present study aimed to investigate the chemical diversity of essential oils of Korean A. annua collected from different locations in Korea by multivariate analysis. For this purpose, the seeds of A. annua were collected from 112 different locations in Korea and were grown under the same environmental conditions. Except for nine individuals which decayed during the cultivation, essential oils were isolated from the aerial parts of 103 A. annua individuals (AEOs) using the steam distillation extraction method, and their chemical compositions were determined by GC-MS analysis. Furthermore, a multivariate analysis was performed to distinguish the difference between 103 individuals of A. annua based on their essential oil compositions. The yield of A. annua essential oils ranged from 0.04 to 1.09% (v\/w). Based on the GC-MS data, A. annua individuals were grouped into six chemotypes such as artemisia ketone, camphor, \u03b2-cubebene, eucalyptol, \u03b1-pinene, and \u03b2-selinene. The multivariate analysis results revealed that Korean A. annua could be largely grouped into three clusters such as artemisia ketone, eucalyptol, and \u03b2-selinene. Among 35 components selected for principal component analysis (PCA), PC1, PC2, and PC3 accounted for 82.55%, 8.74%, and 3.62%, respectively. Although all individuals of A. annua were cultivated under the same environmental conditions, there is an intraspecific chemical diversity that exists within Korean native species.<\/jats:p>","DOI":"10.3390\/molecules28031131","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T03:48:08Z","timestamp":1674445688000},"page":"1131","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Multivariate Analysis of Essential Oil Composition of Artemisia annua L. Collected from Different Locations in Korea"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1310-5472","authenticated-orcid":false,"given":"Minji","family":"Hong","sequence":"first","affiliation":[{"name":"School of Natural Resources and Environmental Science, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minju","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Natural Resources and Environmental Science, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7830-6067","authenticated-orcid":false,"given":"Haejung","family":"Jang","sequence":"additional","affiliation":[{"name":"School of Natural Resources and Environmental Science, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sela","family":"Bo","sequence":"additional","affiliation":[{"name":"School of Natural Resources and Environmental Science, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ponnuvel","family":"Deepa","sequence":"additional","affiliation":[{"name":"School of Natural Resources and Environmental Science, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8638-6025","authenticated-orcid":false,"given":"Kandhasamy","family":"Sowndhararajan","sequence":"additional","affiliation":[{"name":"Department of Botany, Kongunadu Arts and Science College, Coimbatore 641029, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8032-7569","authenticated-orcid":false,"given":"Songmun","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Natural Resources and Environmental Science, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s12272-021-01328-4","article-title":"Phytochemistry and pharmacological activity of the genus Artemisia","volume":"44","author":"Bisht","year":"2021","journal-title":"Arch. Pharm. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"159819","DOI":"10.1155\/2014\/159819","article-title":"Essential oil of Artemisia annua L.: An extraordinary component with numerous antimicrobial properties","volume":"2014","author":"Bilia","year":"2014","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_3","first-page":"927","article-title":"Chemical composition, antioxidant, and antimicrobial activities of the essential oils from Artemisia annua L. growing wild in Tajikistan","volume":"15","author":"Sharopov","year":"2020","journal-title":"Nat. Prod. Commun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"107650","DOI":"10.1016\/j.pharmthera.2020.107650","article-title":"Traditional application and modern pharmacological research of Artemisia annua L","volume":"216","author":"Feng","year":"2020","journal-title":"Pharmacol. Ther."},{"key":"ref_5","unstructured":"Lee, Y.N. (2006). New Flora of Korea, Hyangmunsa Press. (In Korean)."},{"key":"ref_6","unstructured":"National Insititute of Korean History (NIKH) (2022, December 01). The veritable records of the Joseon Dynasty. National Insititute of Korean History, (In Korean)."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.semcancer.2017.02.009","article-title":"From ancient herb to modern drug: Artemisia annua and artemisinin for cancer therapy","volume":"46","author":"Efferth","year":"2017","journal-title":"Semin. Cancer Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1002\/ptr.6733","article-title":"Artemisia annua: Trials are needed for COVID-19","volume":"34","author":"Haq","year":"2020","journal-title":"Phytother. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zhang, X., Zhao, Y., Guo, L., Qiu, Z., Huang, L., and Qu, X. (2017). Differences in chemical constituents of Artemisia annua L. from different geographical regions in China. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0183047"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2542","DOI":"10.3390\/molecules17032542","article-title":"The Artemisia L. Genus: A review of bioactive essential oils","volume":"17","author":"Abad","year":"2012","journal-title":"Molecules"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1080\/10412905.2003.9712131","article-title":"Essential oil constituents of Artemisia annua during different growth periods at monsoon conditions of subtropical north Indian plains","volume":"15","author":"Bagchi","year":"2003","journal-title":"J. Essent. Oil Res."},{"key":"ref_12","first-page":"243","article-title":"Biological activities of sweet wormwood (Artemisia annua L.)","volume":"10","author":"Hong","year":"2021","journal-title":"Korean J. Weed Turf. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.indcrop.2011.07.024","article-title":"Chemical composition and antioxidant and antimicrobial activity of essential oil of Artemisia annua L. from Bosnia","volume":"37","author":"Vidic","year":"2012","journal-title":"Ind. Crop. Prod."},{"key":"ref_14","first-page":"539","article-title":"Anti-adipocyte differentiation activity and chemical composition of essential oil from Artemisia annua","volume":"11","author":"Hwang","year":"2016","journal-title":"Nat. Prod. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Hong, M.J., Deepa, P., Lee, K.Y., Kim, K., Sowndhararajan, K., and Kim, S. (2022). Chemical diversity of essential oils from Korean native populations of Agastache rugosa (Korean Mint). Molecules, 27.","DOI":"10.3390\/molecules27196341"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kim, M.J., Sowndhararajan, K., and Kim, S. (2022). Variations in the chemical composition of essential oils in native populations of Korean thyme, Thymus quinquecostatus Celak. Molecules, 27.","DOI":"10.3390\/molecules27217203"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2668","DOI":"10.1080\/14786419.2018.1548461","article-title":"Composition and antioxidant activity of the essential oil of Artemisia annua L","volume":"34","author":"Zhigzhitzhapova","year":"2019","journal-title":"Nat. Prod. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.fct.2013.04.016","article-title":"Toxic essential oils. Part II: Chemical, toxicological, pharmacological and microbiological profiles of Artemisia annua L. volatiles","volume":"58","year":"2013","journal-title":"Food Chem. Toxicol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1002\/(SICI)1099-1026(199707)12:4<241::AID-FFJ641>3.0.CO;2-Z","article-title":"Variation in the essential oil composition of Artemisia annua L. of different origin cultivated in Finland","volume":"12","author":"Holm","year":"1997","journal-title":"Flavour Frag. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.21273\/HORTSCI.48.10.1288","article-title":"Distillation time effect on essential oil yield, composition, and antioxidant capacity of sweet sagewort (Artemisia annua L.) oil","volume":"48","author":"Zheljazkov","year":"2013","journal-title":"HortScience"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7603","DOI":"10.3390\/molecules15117603","article-title":"The biosynthesis of artemisinin (Qinghaosu) and the phytochemistry of Artemisia annua L. (Qinghao)","volume":"15","author":"Brown","year":"2010","journal-title":"Molecules"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s11418-006-0112-9","article-title":"Essential oils of petal, leaf and stem of the antimalarial plant Artemisia annua","volume":"61","author":"Goel","year":"2007","journal-title":"J. Nat. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"103960","DOI":"10.1016\/j.bse.2019.103960","article-title":"Essential oil composition of five Artemisia (Compositae) species in regards to chemophenetics","volume":"87","author":"Giweli","year":"2019","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_24","first-page":"355","article-title":"In vitro effects of essential oils from the aerial parts of Artemisia annua L. against antibiotic-susceptible and -resistant strains of Salmenella typhimurium","volume":"51","author":"Shin","year":"2007","journal-title":"J. Pharm. Soc. Korea"},{"key":"ref_25","first-page":"35","article-title":"Secondary metabolites of Artemisia annua and their biological activity","volume":"80","author":"Bhakuni","year":"2001","journal-title":"Curr. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1080\/10412905.1991.9697903","article-title":"Characterization of the essential oil of Artemisia annua L","volume":"3","author":"Charles","year":"1991","journal-title":"J. Essent. Oil Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1080\/14786419.2019.1599887","article-title":"Essential oil from Artemisia annua aerial parts: Composition and repellent activity against two storage pests","volume":"35","author":"Liu","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.2478\/V10133-010-0030-6","article-title":"Composition of Artemisia annua essential oil obtained from species growing wild in Bulgaria","volume":"24","author":"Tzenkova","year":"2010","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.1002\/cbdv.201400340","article-title":"Chemical Composition and Antipathogenic Activity of Artemisia annua Essential Oil from Romania","volume":"12","author":"Marinas","year":"2015","journal-title":"Chem Biodivers."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1055\/s-0043-118912","article-title":"Liquid and Vapor-Phase Activity of Artemisia annua Essential Oil against Pathogenic Malassezia spp","volume":"84","author":"Santomauro","year":"2018","journal-title":"Planta Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"101849","DOI":"10.1016\/j.jddst.2020.101849","article-title":"Artemisia annua essential oil extraction, characterization, and incorporation in nanoliposomes, smart drug delivery systems against Candida species","volume":"59","author":"Risaliti","year":"2020","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"W\u00eaglarz, Z., Kosakowska, O., Przyby\u0142, J.L., Pi\u00f3ro-Jabrucka, E., and Baczek, K. (2020). The quality of Greek oregano (O. vulgare L. subsp. hirtum (Link) Ietswaart) and common oregano (O. vulgare L. subsp. vulgare) Cultivated in the temperate climate of Central Europe. Foods, 9.","DOI":"10.3390\/foods9111671"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"265","DOI":"10.4103\/0250-474X.106074","article-title":"Constituents of Artemisia gmelinii Weber ex Stechm. from Uttarakhand Himalaya: A Source of Artemisia Ketone","volume":"74","author":"Haider","year":"2012","journal-title":"Indian J. Pharm. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5434","DOI":"10.3390\/molecules18055434","article-title":"Camphor\u2014A fumigant during the Black Death and a coveted fragrant wood in ancient Egypt and Babylon\u2014A review","volume":"18","author":"Chen","year":"2013","journal-title":"Molecules"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ivanov, M., Kannan, A., Stojkovi\u0107, D.S., Glamo\u010dlija, J., Calhelha, R.C., Ferreira, I.C.F.R., Sanglard, D., and Sokovi\u0107, M. (2021). Camphor and Eucalyptol-Anticandidal Spectrum, Antivirulence Effect, Efflux Pumps Interference and Cytotoxicity. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22020483"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1080\/10286020.2020.1839432","article-title":"1,8-Cineole: A review of source, biological activities, and application","volume":"23","author":"Cai","year":"2021","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"112857","DOI":"10.1016\/j.phytochem.2021.112857","article-title":"\u03b1-Pinene: A never-ending story","volume":"190","author":"Allenspach","year":"2021","journal-title":"Phytochemistry"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1002\/jsfa.8563","article-title":"Comparative analysis of juice volatiles in selected mandarins, mandarin relatives and other citrus genotypes","volume":"98","author":"Yu","year":"2018","journal-title":"J. Sci. Food Agric."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Vella, F.M., Calandrelli, R., Cautela, D., Fiume, I., Pocsfalvi, G., and Laratta, B. (2020). Chemometric Screening of Fourteen Essential Oils for Their Composition and Biological Properties. Molecules, 25.","DOI":"10.3390\/molecules25215126"},{"key":"ref_40","unstructured":"Adams, R.P. (2007). Identification of Essential Oil Components by Gas Chromatography\/Mass Spectrometry, Allured Publishing Co., Ltd."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/3\/1131\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:13:58Z","timestamp":1760120038000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/3\/1131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,23]]},"references-count":40,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["molecules28031131"],"URL":"https:\/\/doi.org\/10.3390\/molecules28031131","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,23]]}}}