{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:48:06Z","timestamp":1760147286900,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T00:00:00Z","timestamp":1674604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Funds","award":["2018-1828-0002370"],"award-info":[{"award-number":["2018-1828-0002370"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Casearia coriacea Vent., an endemic plant from the Mascarene Islands, was investigated following its antiplasmodial potentialities highlighted during a previous screening. Three clerodane diterpene compounds were isolated and identified as being responsible for the antiplasmodial activity of the leaves of the plant: caseamembrin T (1), corybulosin I (2), and isocaseamembrin E (3), which exhibited half maximal inhibitory concentrations (IC50) of 0.25 to 0.51 \u00b5g\/mL. These compounds were tested on two other parasites, Leishmania\u00a0mexicana mexicana and Trypanosoma brucei brucei, to identify possible selectivity in one of them. Although these products possess both antileishmanial and antitrypanosomal properties, they displayed selectivity for the malaria parasite, with a selectivity index between 6 and 12 regarding antitrypanosomal activity and between 25 and 100 regarding antileishmanial activity. These compounds were tested on three cell lines, breast cancer cells MDA-MB-231, pulmonary adenocarcinoma cells A549, and pancreatic carcinoma cells PANC-1, to evaluate their selectivity towards Plasmodium. This has not enabled us to establish selectivity for Plasmodium, but has revealed the promising activity of compounds 1\u20133 (IC50 &lt; 2 \u00b5g\/mL), particularly against pancreatic carcinoma cells (IC50 &lt; 1 \u00b5g\/mL). The toxicity of the main compound, caseamembrin T (1), was then evaluated on zebrafish embryos to extend our cytotoxicity study to normal, non-cancerous cells. This highlighted the non-negligible toxicity of caseamembrin T (1).<\/jats:p>","DOI":"10.3390\/molecules28031197","type":"journal-article","created":{"date-parts":[[2023,1,26]],"date-time":"2023-01-26T01:30:30Z","timestamp":1674696630000},"page":"1197","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Bioactive Clerodane Diterpenoids from the Leaves of Casearia coriacea Vent"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4052-6336","authenticated-orcid":false,"given":"Allison","family":"Ledoux","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, CIRM, University of Li\u00e8ge, Avenue Hippocrate 15, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Hamann","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, CIRM, University of Li\u00e8ge, Avenue Hippocrate 15, 4000 Li\u00e8ge, Belgium"},{"name":"Laboratory of Biology of Tumor and Development, GIGA\/CIRM, University of Li\u00e8ge, Avenue Hippocrate 15, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0350-6640","authenticated-orcid":false,"given":"Olivier","family":"Bonnet","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, CIRM, University of Li\u00e8ge, Avenue Hippocrate 15, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kateline","family":"Jullien","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, CIRM, University of Li\u00e8ge, Avenue Hippocrate 15, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2278-6206","authenticated-orcid":false,"given":"Jo\u00eblle","family":"Quetin-Leclercq","sequence":"additional","affiliation":[{"name":"Pharmacognosy Research Group, Louvain Drug Research Institute, LDRI, Universit\u00e9 Catholique de Louvain, UCLouvain, Avenue E. Mounier, B1 72.03, B-1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alembert","family":"Tchinda","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, CIRM, University of Li\u00e8ge, Avenue Hippocrate 15, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Smadja","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, Universit\u00e9 de R\u00e9union, Avenue Ren\u00e9 Cassin 15, BP 7151, 97715 Saint-Denis, La R\u00e9union, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5188-0704","authenticated-orcid":false,"given":"Anne","family":"Gauvin-Bialecki","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, Universit\u00e9 de R\u00e9union, Avenue Ren\u00e9 Cassin 15, BP 7151, 97715 Saint-Denis, La R\u00e9union, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2193-0800","authenticated-orcid":false,"given":"Erik","family":"Maquoi","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Tumor and Development, GIGA\/CIRM, University of Li\u00e8ge, Avenue Hippocrate 15, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0770-9990","authenticated-orcid":false,"given":"Michel","family":"Fr\u00e9d\u00e9rich","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, CIRM, University of Li\u00e8ge, Avenue Hippocrate 15, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,25]]},"reference":[{"key":"ref_1","unstructured":"(2023, January 16). 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